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Fekrazad, Reza, Tondnevi, Farbod, Abolhasani, Mohamad Mahdi. (1401). In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration. سامانه مدیریت نشریات علمی, 12(4), 395-402. doi: 10.31661/jbpe.v0i0.1188
Reza Fekrazad; Farbod Tondnevi; Mohamad Mahdi Abolhasani. "In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration". سامانه مدیریت نشریات علمی, 12, 4, 1401, 395-402. doi: 10.31661/jbpe.v0i0.1188
Fekrazad, Reza, Tondnevi, Farbod, Abolhasani, Mohamad Mahdi. (1401). 'In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration', سامانه مدیریت نشریات علمی, 12(4), pp. 395-402. doi: 10.31661/jbpe.v0i0.1188
Fekrazad, Reza, Tondnevi, Farbod, Abolhasani, Mohamad Mahdi. In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration. سامانه مدیریت نشریات علمی, 1401; 12(4): 395-402. doi: 10.31661/jbpe.v0i0.1188

In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration

Journal of Biomedical Physics and Engineering
مقاله 9، دوره 12، شماره 4، آبان 2022، صفحه 395-402    اصل مقاله (1.4 M)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.1188
نویسندگان
Reza Fekrazad* 1، 2؛ Farbod Tondnevi3؛ Mohamad Mahdi Abolhasani4
1DDS, Department of Periodontology, Dental Faculty, AJA University of Medical Sciences, Tehran, Iran
22DDS, International Network for Photo Medicine and Photo Dynamic Therapy (INPMPDT), Universal Scientific Education and Research Network (USERN), Tehran, Iran
3PhD, Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
4PhD, Department of Nanotechnology & Advanced Materials, Materials & Energy Research Center (MERC), Karaj, Iran
چکیده
Background: Many patients lose their organs or tissues due to disease, trauma, or a variety of genetic disorders. Tissue engineering is a multidisciplinary science to regenerate or restore tissue or organ function and an appropriate scaffold is the first and certainly a crucial step in tissue engineering strategies. 
Objective: The purpose of this study is to fabricate and evaluate the in-vitro response of porous nano Polycaprolactone (PCL)/ chitosan/ multi-wall carbon nanotube (MWCNTs) scaffold for tissue regeneration.
Material and Methods: In this experimental research, a novel scaffold containing MWCNTs in polycaprolactone/chitosan nanofibrous scaffold was synthesized by electrospinning technique. 
Results: According to scanning electron microscopy SEM images, by increasing the number of MWCNT in the scaffold by 2%, the average diameter decreased significantly for fabricated scaffolds with 5% MWCNTs. Based on the results, the scaffolds plunged from submicron to nanoscale fibers at about 80 nm. In addition, by adding more MWCNT to the nanofibrous scaffold, the biodegradation rate was decreased by 32%. However, mechanical characterization demonstrates that the higher level of MWCNT increases young modulus by 96%, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay illustrated that MWCNTs could enhance bioactivity and cell- scaffold relationship in addition to alkaline phosphatase (ALP).  
Conclusion: MWCNT significantly improves the physical and mechanical properties of fabricated scaffolds and in-vitro assessment demonstrated that the prepared nanofibrous scaffold containing 4% MWCNT could be a very useful biocompatible material for tissue engineering.
کلیدواژه‌ها
Multi-Wall Carbon Nano Tube؛ Electrospun Scaffold؛ Nanofibers؛ Chitosan؛ Polycaprolactone؛ Tissue Engineering
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