LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,149
تعداد مقالات10,518
تعداد مشاهده مقاله45,415,519
تعداد دریافت فایل اصل مقاله11,291,323
Entezari, Sarvin, Moezzighadim, Nahid, Lawaf, Shirin, Azizi, Arash. (1401). In vitro Effect of Photodynamic Therapy with Curcumin and Methylene Blue Photosensitizers on Staphylococcus Aureus. سامانه مدیریت نشریات علمی, 23(Supplement-September-2022), 387-392. doi: 10.30476/dentjods.2021.90146.1470
Sarvin Entezari; Nahid Moezzighadim; Shirin Lawaf; Arash Azizi. "In vitro Effect of Photodynamic Therapy with Curcumin and Methylene Blue Photosensitizers on Staphylococcus Aureus". سامانه مدیریت نشریات علمی, 23, Supplement-September-2022, 1401, 387-392. doi: 10.30476/dentjods.2021.90146.1470
Entezari, Sarvin, Moezzighadim, Nahid, Lawaf, Shirin, Azizi, Arash. (1401). 'In vitro Effect of Photodynamic Therapy with Curcumin and Methylene Blue Photosensitizers on Staphylococcus Aureus', سامانه مدیریت نشریات علمی, 23(Supplement-September-2022), pp. 387-392. doi: 10.30476/dentjods.2021.90146.1470
Entezari, Sarvin, Moezzighadim, Nahid, Lawaf, Shirin, Azizi, Arash. In vitro Effect of Photodynamic Therapy with Curcumin and Methylene Blue Photosensitizers on Staphylococcus Aureus. سامانه مدیریت نشریات علمی, 1401; 23(Supplement-September-2022): 387-392. doi: 10.30476/dentjods.2021.90146.1470

In vitro Effect of Photodynamic Therapy with Curcumin and Methylene Blue Photosensitizers on Staphylococcus Aureus

Journal of Dentistry
مقاله 7، دوره 23، Supplement-September-2022، آذر 2022، صفحه 387-392    اصل مقاله (596.05 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/dentjods.2021.90146.1470
نویسندگان
Sarvin Entezari1؛ Nahid Moezzighadim2؛ Shirin Lawaf3؛ Arash Azizi* 2
1Dentist, Faculty of Dentistry, Tehran Medical Sciences. Islamic Azad University, Tehran, Iran.
2Dept. of Oral Medicine, Faculty of Dentistry, Tehran Medical Sciences. Islamic Azad University, Tehran, Iran.
3Dept. of Prosthodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
چکیده
Statement of the Problem: Staphylococcus aureus (S.A) can colonize in the skin, nasal cavity, and oral cavity. In the oral cavity, it can cause dental caries and periodontal disease. Mouthwashes can be used as an adjunct to mechanical plaque control methods to decrease the load of oral microorganisms. Chlorhexidine (CHX) is a commonly used antimicrobial mouthwash with side effects such as changing the sense of taste, tooth discoloration, oral mucosal burning, allergy, and xerostomia. It also has adverse systemic effects, if swallowed.
Purpose: This study aimed to assess the effect of photodynamic therapy (PDT) with curcumin and methylene blue (MB) photosensitizers and different laser parameters on S.A colony count.
Materials and Method: In this in vitro experimental study, 99 samples of standard-strain S.A were subjected to PDT with curcumin and MB photosensitizers with/without irradiation of 660 and 445 nm laser with different exposure parameters, and CHX in 9 groups (n=11). The samples were cultured in microplates containing Mueller-Hinton agar, and the number of colony forming units (CFUs) was counted after 24 h of incubation at 37°C. Data were analyzed by the Kruskal-Wallis and Dunn tests.
Results: The minimum colony count was noted in CHX group (CFUs=0) followed by MB and 660nm diode laser group irradiated for 100 s (CFUs=147.2727±169.35707). The difference in this respect was significant between MB+660nm diode laser for 100 s and other groups (p< 0.05) except for the MB + 660 nm diode laser for 60 s group.
Conclusion: CHX is superior to laser for elimination of S.A. However, PDT with 660 nm diode laser + MB has considerable antimicrobial efficacy against S.A; increasing the duration of laser irradiation enhances the antimicrobial effect.
کلیدواژه‌ها
Staphylococcus Aureus؛ Photodynamic Therapy؛ Photochemotherapy؛ Photosensitizing agent؛ Photosensitivity disorders؛ Curcumin؛ Methylene Blue
سایر فایل های مرتبط با مقاله
مراجع
  • Zan R, Kutlu G, Hubbezoglu I, Sumer Z, Tunc T, Mutlu Z. Bactericidal effects of various irrigation solutions against Staphylococcus Aureus in human root canal. J Istanb Univ Fac Dent. 2015; 49: 19-26.
  • Smith AJ, Jackson MS, Bagg J. The ecology of Staphylococcus species in the oral cavity. J Med Microbiol. 2001; 50: 940-946.
  • Murdoch FE, Sammons RL, Chapple IL. Isolation and characterization of subgingival staphylococci from periodontitis patients and controls. Oral Dis. 2004; 10: 155-162.
  • Wilson M. Bacterial biofilms and human disease. Sci Prog. 2001; 84: 235-254.
  • Chye RM, Perrotti V, Piattelli A, Iaculli F, Quaranta A. Effectiveness of different commercial chlorhexidine-based mouthwashes after periodontal and implant surgery: a systematic review. Implant Dent. 2019; 28: 74-85.
  • Pires JR, Rossa*Junior C, Pizzolitto AC. In vitro antimicrobial efficiency of a mouthwash containing triclosan/gantrez and sodium bicarbonate. Braz Oral Res. 2007; 21: 342-347.
  • Konopka KR, Goslinski TO. Photodynamic therapy in dentistry. J Dent Res. 2007; 86: 694-707.
  • Paschoal MA, Tonon CC, Spolidório DM, Bagnato VS, Giusti JS, Santos-Pinto L. Photodynamic potential of curcumin and blue LED against Streptococcus mutans in a planktonic culture. Photodiagnosis Photodyn Ther. 2013; 10: 313-319.
  • Oda DF, Duarte MA, Andrade FB, Moriyama LT, Bagnato VS, de Moraes IG. Antimicrobial action of photodynamic therapy in root canals using LED curing light, curcumin and carbopol gel. Int Endod J. 2019; 52: 1010-1019.
  • Bliss JM, Bigelow CE, Foster TH, Haidaris CG. Susceptibility of Candida species to photodynamic effects of photofrin. Antimicrob Agents Chemother. 2004; 48: 2000-2006.
  • Fontana CR, Abernethy AD, Som S, Ruggiero K, Doucette S, Marcantonio RC, et al. The antibacterial effect of photodynamic therapy in dental plaque‐derived biofilms. J Periodontal Res. 2009; 44: 751-759.
  • Foschi F, Fontana CR, Ruggiero K, Riahi R, Vera A, Doukas AG, et al. Photodynamic inactivation of Enterococcus faecalis in dental root canals in vitro. Lasers Surg Med. 2007; 39: 782-787.
  • Azizi A, Shademan S, Rezai M, Rahimi A, Lawaf S. Effect of photodynamic therapy with two photosensitizers on Streptococcus mutants: in vitro Photodiagnosis Photodyn Ther. 2016; 16: 66-71.
  • Azizi A, Mousavian S, Taheri S, Lawaf S, Gonoudi E, Rahimi A. Comparison of the antimicrobial efficacy of photodynamic therapy with two mediators against Lactobacillus acidophilus in vitro. Photodiagnosis Photodyn Ther. 2018; 21: 357-362.
  • Azizi A, Amirzadeh Z, Rezai M, Lawaf S, Rahimi A. Effect of photodynamic therapy with two photosensitizers on Candida albicans. J Photochem Photobiol B. 2016; 158: 267-273.
  • Pourhajibagher M, Chiniforush N, Shahabi S, Palizvani M, Bahador A. Antibacterial and Antibiofilm Efficacy of Antimicrobial Photodynamic Therapy Against Intracanal Enterococcus faecalis: An in vitro Comparative Study with Traditional Endodontic Irrigation Solutions. J Dent (Tehran). 2018; 15: 197.
  • Azizi A, Shohrati P, Goudarzi M, Lawaf S, Rahimi A. Comparison of the effect of photodynamic therapy with curcumin and methylene Blue on streptococcus mutans bacterial colonies. Photodiagnosis Photodyn Ther. 2019; 27: 203-209.
  • Nemezio MA, de*Souza Farias SS, Borsatto MC, Aires CP, Corona SA. Effect of methylene blue-induced photodynamic therapy on a Streptococcus mutans biofilm model. Photodiagnosis Photodyn Ther. 2017; 20: 234-237.
  • Giusti JS, Santos-Pinto L, Pizzolito AC, Helmerson K, Carvalho-Filho E, Kurachi C, et al. Antimicrobial photodynamic action on dentin using a light-emitting diode light source. Photomed Laser Surg. 2008; 26: 281-287.
  • Dovigo LN, Carmello JC, de*Souza Costa CA, Vergani CE, Brunetti IL, Bagnato VS, et al. Curcuminmediated photodynamic inactivation of Candida albicans in a murine model of oral candidiasis. Medical Mycology. 2013; 51: 243-251.
آمار
تعداد مشاهده مقاله: 2,409
تعداد دریافت فایل اصل مقاله: 2,060


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب