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Akafzadeh, Negar, Baghaei, Fereshteh, Derakhshan, Samira, Moradzadeh Khiavi, Monir, Kharazifard, Mohamadjavad, Aminishakib, Pouyan. (1401). Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia. سامانه مدیریت نشریات علمی, 23(Supplement-September-2022), 377-382. doi: 10.30476/dentjods.2021.91032.1546
Negar Akafzadeh; Fereshteh Baghaei; Samira Derakhshan; Monir Moradzadeh Khiavi; Mohamadjavad Kharazifard; Pouyan Aminishakib. "Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia". سامانه مدیریت نشریات علمی, 23, Supplement-September-2022, 1401, 377-382. doi: 10.30476/dentjods.2021.91032.1546
Akafzadeh, Negar, Baghaei, Fereshteh, Derakhshan, Samira, Moradzadeh Khiavi, Monir, Kharazifard, Mohamadjavad, Aminishakib, Pouyan. (1401). 'Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia', سامانه مدیریت نشریات علمی, 23(Supplement-September-2022), pp. 377-382. doi: 10.30476/dentjods.2021.91032.1546
Akafzadeh, Negar, Baghaei, Fereshteh, Derakhshan, Samira, Moradzadeh Khiavi, Monir, Kharazifard, Mohamadjavad, Aminishakib, Pouyan. Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia. سامانه مدیریت نشریات علمی, 1401; 23(Supplement-September-2022): 377-382. doi: 10.30476/dentjods.2021.91032.1546

Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia

Journal of Dentistry
مقاله 5، دوره 23، Supplement-September-2022، آذر 2022، صفحه 377-382    اصل مقاله (671.2 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/dentjods.2021.91032.1546
نویسندگان
Negar Akafzadeh1؛ Fereshteh Baghaei2؛ Samira Derakhshan3؛ Monir Moradzadeh Khiavi3؛ Mohamadjavad Kharazifard4؛ Pouyan Aminishakib* 3
1Postgraduate Student of Pediatric Dentistry, Department of Pediatric Dentistry, School of Dentistry, Qazvin University of Medical Sciences, Qazvin , Iran
2Retired Professor, Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
3Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
4Researcher, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
چکیده
Statement of the Problem: Oral epithelial dysplasia (OED) is a common potentially malignant lesion of oral cavity that should be managed to prevent likely malignant transformation.
Purpose: Here, we present a combination of binary grading system with complementary immunohistochemical (IHC) staining for Ki67 biomarker to provide a reproducible OED grading system.
Materials and Method: In this cross-sectional study, seventy out of one hundred OED specimens, which were accompanied by IHC stained microscopic slides for Ki67 antigen were evaluated by four independent oral pathologists. Both three-tier and binary grading systems based on WHO microscopic criteria were employed , blindly in a four-step method with at least two-week interval between each observation. Intra- and inter-observational reliability was assessed using Kappa statistical analysis.
Results: OED diagnosis based on binary system showed significant intra-observer reliability comparing to three-tier system without biomarker. Moreover, OED diagnosis based on binary system using Ki67 biomarker showed significant inter-observer reliability comparing to diagnosis in three tier system and based on binary system without Ki67 biomarker showed significant inter-observer reliability comparing to diagnosis based on three-tier system without Ki67.
Conclusion: Here, we found that application of IHC staining for Ki67 biomarker in binary system might provide a more reliable grading method for oral pathologist form different educational background.
کلیدواژه‌ها
Ki-67 Antigen؛ Precancerous Conditions؛ Carcinoma in Situ
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مراجع
  • Van*Monsjou HS, Balm AJ, Van*den*Brekel MM, Wreesmann VB. Oropharyngeal squamous cell carcinoma: a unique disease on the rise? Oral Oncol. 2010; 46: 780-785.
  • Ausoni S, Boscolo-Rizzo P, Singh B, Da*Mosto MC, Spinato G, Tirelli G, et al. Targeting cellular and molecular drivers of head and neck squamous cell carcinoma: current options and emerging perspectives. Cancer Metastasis Rev. 2016; 35: 413-426.
  • Ho PS, Chen PL, Warnakulasuriya S, Shieh TY, Chen YK, Huang IY. Malignant transformation of oral potentially malignant disorders in males: a retrospective cohort study. BMC Cancer. 2009; 9: 1-7.
  • Lumerman H, Freedman P, Kerpel S. Oral epithelial dysplasia and the development of invasive squamous cell carcinoma. Oral Surg. Oral Med Oral Pathol Oral Radiol. 1995; 79: 321-329.
  • Warnakulasuriya S. Histological grading of oral epithelial dysplasia: revisited. J Pathol. 2001; 194: 294-297.
  • Fleskens S, Slootweg P. Grading systems in head and neck dysplasia: their prognostic value, weaknesses and utility. Head Neck Oncol. 2009; 1: 11.
  • Krishnan L, Karpagaselvi K, Kumarswamy J, Sudhendra U, Santosh K, Patil A. Inter-and intra-observer variability in three grading systems for oral epithelial dysplasia. J Oral Maxillofac Pathol. 2016; 20: 261.
  • Reibel J. Prognosis of oral pre-malignant lesions: significance of clinical, histopathological and molecular biologiccal characteristics. Crit Rev Oral Biol Med. 2003;14:47-62.
  • Warnakulasuriya S, Reibel J, Bouquot J, Dabelsteen E. Oral epithelial dysplasia classification systems: predictive value, utility, weaknesses and scope for improvement. J Oral Maxillofac Pathol. 2008; 37: 127-133.
  • Speight PM. Update on oral epithelial dysplasia and progression to cancer. Head Neck Pathol. 2007; 1: 61-66.
  • Ranganathan K, Kavitha L. Oral epithelial dysplasia: Classifications and clinical relevance in risk assessment of oral potentially malignant disorders. J Oral Maxillofac Pathol. 2019; 23: 19-27.
  • Abdulmajeed AA, Farah CS. Can immunohistochemistry serve as an alternative to subjective histopathological diagnosis of oral epithelial dysplasia? Biomarker Cancer. 2013; 5: 49-60.
  • Dwivedi N, Chandra S, Kashyap B, Raj V, Agarwal A. Suprabasal expression of Ki-67 as a marker for the severity of oral epithelial dysplasia and oral squamous cell carcinoma. Contemp Clin Dent. 2013; 4: 7-12.
  • Viera AJ, Garrett JM. Understanding interobserver agreement: the kappa statistic. Fam Med. 2005; 37: 360-363.
  • Kujan O, Khattab A, Oliver RJ, Roberts SA, Thakker N, Sloan P. Why oral histopathology suffers inter-observer variability on grading oral epithelial dysplasia: an attempt to understand the sources of variation. Oral Oncol. 2007; 43: 224-231.
  • Nankivell P, Williams H, Matthews P, Suortamo S, Snead D, McConkey C, et al. The binary oral dysplasia grading system: validity testing and suggested improvement. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013; 115: 87-94.
  • Geetha KM, Leeky M, Narayan TV, Sadhana S, Saleha J. Grading of oral epithelial dysplasia: Points to ponder. J Oral Maxillofac Pathol. 2015; 19: 198-204.
  • Gadbail AR, Chaudhary MS, Sarode SC, Gawande M, Korde S, Tekade SA, et al. Ki67, CD105 and alpha-SMA expressions better relate the binary oral epithelial dysplasia grading system of World Health Organization. J Oral Maxillofac Pathol. 2017; 46: 921-927.
  • Brown D, Gatter K. Ki67 protein: the immaculate deception? Histopathology. 2002; 40: 2-11.
  • Fischer DJ, Epstein JB, Morton Jr*TH, Schwartz SM. Interobserver reliability in the histopathologic diagnosis of oral pre‐malignant and malignant lesions. J Oral Maxillofac Pathol. 2004; 33: 65-70.
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