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Kiani, Marjan, Zabihi, Ebrahim, Nafarzadeh, Shima, Nouri, Hamidreza, Bijani, Ali, Seyedmajidi, Maryam. (1401). Anti-Cancer Effect of Bromelain and Its Combination with Cisplatin on HN5 Cell Line (Squamous Cell Carcinoma). سامانه مدیریت نشریات علمی, 23(3), 257-265. doi: 10.30476/dentjods.2021.89577.1478
Marjan Kiani; Ebrahim Zabihi; Shima Nafarzadeh; Hamidreza Nouri; Ali Bijani; Maryam Seyedmajidi. "Anti-Cancer Effect of Bromelain and Its Combination with Cisplatin on HN5 Cell Line (Squamous Cell Carcinoma)". سامانه مدیریت نشریات علمی, 23, 3, 1401, 257-265. doi: 10.30476/dentjods.2021.89577.1478
Kiani, Marjan, Zabihi, Ebrahim, Nafarzadeh, Shima, Nouri, Hamidreza, Bijani, Ali, Seyedmajidi, Maryam. (1401). 'Anti-Cancer Effect of Bromelain and Its Combination with Cisplatin on HN5 Cell Line (Squamous Cell Carcinoma)', سامانه مدیریت نشریات علمی, 23(3), pp. 257-265. doi: 10.30476/dentjods.2021.89577.1478
Kiani, Marjan, Zabihi, Ebrahim, Nafarzadeh, Shima, Nouri, Hamidreza, Bijani, Ali, Seyedmajidi, Maryam. Anti-Cancer Effect of Bromelain and Its Combination with Cisplatin on HN5 Cell Line (Squamous Cell Carcinoma). سامانه مدیریت نشریات علمی, 1401; 23(3): 257-265. doi: 10.30476/dentjods.2021.89577.1478

Anti-Cancer Effect of Bromelain and Its Combination with Cisplatin on HN5 Cell Line (Squamous Cell Carcinoma)

Journal of Dentistry
مقاله 2، دوره 23، شماره 3 - شماره پیاپی 76، آذر 2022، صفحه 257-265    اصل مقاله (895.55 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/dentjods.2021.89577.1478
نویسندگان
Marjan Kiani1؛ Ebrahim Zabihi2؛ Shima Nafarzadeh3؛ Hamidreza Nouri4؛ Ali Bijani5؛ Maryam Seyedmajidi* 6
1Student Research Committee, Postgraduate student of oral and maxillofacial pathology, Babol University of Medical Sciences, Babol, Iran.
2Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
3Oral Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
4Dept. of Immunology, Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
5Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
6Dental Materials Research Center, Health Institute, Babol University of Medical Sciences, Babol, Iran.
چکیده
Statement of the Problem: Squamous cell carcinoma (SCC) comprises over 90% of oral malignancies. Cisplatin, as a selective chemotherapy agent to treat SCC, has many side effects despite its high effectiveness. There are some studies on the effects of bromelain derived from pineapple stems on different malignancies.
Purpose: The aim of this study was to investigate the effect of bromelain alone and in combination with Cisplatin on oral squamous cell carcinoma (OSCC) and fibroblast cell lines.
Materials and Method: In this interventional study, the HN5 cell line of OSCC and fibroblast cell line were treated with different concentrations of bromelain alone and in combination with cisplatin. Cell viability test was performed after 24, 48 and 72 hours using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. In the final stage, the drug-treated cells underwent flow cytometry to assess apoptosis patterns. Data were analyzed using SPSS 17, ANOVA (for general comparison of groups) and LSD post hoc tests (for comparison two groups). p< 0.05 was considered statistically significant.
Results: The findings suggested that although bromelain showed toxic effects on HN5 cancer cells, its combination with Cisplatin resulted in little improvement in its effectiveness. Bromelain alone and in combination with Cisplatin presented cytotoxic effects against fibroblasts, which depended on the dosage and time exposure (p˂ 0.05). The flow cytometry results did not support the superior effect of the combination of two medications over Cisplatin alone (p> 0.05).
Conclusion: According to the findings, although adding bromelain to Cisplatin reduced toxicity on normal tissues, the combination of these two drugs did not increase the anticancer effect of Cisplatin. Thus, bromelain in combination with Cisplatin is not recommended as an adjuvant drug for OSCC.
کلیدواژه‌ها
Apoptosis؛ Bromelain؛ Cisplatin؛ Fibroblast؛ Oral squamous cell carcinoma
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  • Silva BS, Yamamoto FP, Pontes FS, Cury SE, Fonseca FP, Pontes HA, et al. TWIST and p-Akt immunoexpression in normal oral epithelium, oral dysplasia and in oral squamous cell carcinoma. Med Oral Patol Oral Cir Bucal. 2012; 17: 29-34.
  • Neville BW, Damm DD, Allen CM, Chi AC. Oral and maxillofacial Pathology. 3th ed. Elsevier: Missouri; 20 p.374-389.
  • Seyedmajidi M, Shafaee S, Siadati S, Khorasani M, Bijani A, Ghasemi N. Cyclo-oxygenase-2 expression in oral squamous cell carcinoma. J Cancer Res Ther. 2014; 10: 1024-1029.
  • Desoize B, Madoulet C. Particular aspects of platinum compounds used at present in cancer treatment. Crit Rev Oncol Hematol. 2002; 42: 317-325.
  • Rosenberg B, Vancamp L, Krigas T. Inhibition of cell division in escherichia coli by electrolysis products from a platinum electrode. Nature. 1965; 205: 698–699.
  • Wang D, Lippard SJ. Cellular processing of platinum anticancer drug. Nat Rev Drug Discov. 2005;4: 307-320.
  • Michaelis M, Bliss J, Arnold SC, Hinsch N, Rothweiler F, Deubzer He, et al. Cisplatin-resistant neuroblastoma cells express enhanced levels of epidermal growth factor receptor (EGFR) and are sensitive to treatment with EGFR-specific toxins. Clin Cancer Res. 2008; 14: 6531-6537.
  • Bourhis J. New approaches to enhance chemotherapy in SCCHN. Ann Oncol. 2005; 16: 20-24.
  • Desoize B, Madoulet C. Particular aspects of platinum compounds used at present in cancer treatment. Crit Rev Oncol Hematol. 2002; 42: 317-325.
  • Florea AM, Büsselberg D. Cisplatin as an anti-tumor drug: cellular mechanisms of activity, drug resistance and induced side effects. Cancers. 2011; 3: 1351-1371.
  • Uccelli A, Moretta L, Pistoia V. Mesenchymal stem cells in health and disease. Nat Rev Immunol. 2008; 8: 726-736.
  • Mondal S, Bhattacharya S, Pandey J, Biswas M. Evaluation of acute anti-inflammatory effect of Ananas Comosus leaf extract in Rats. Pharmocologyonline. 2011; 3: 1312-1315.
  • Bhattacharyya B, Barun K. Bromelain: an overview. Natural Product Radiance. 2008; 7: 359-363.
  • Chobotova K, Vernallis A, Majid A. Bromelain’s activity and potential as an anti-cancer agent: current evidence and perspectives. Cancer Letters. 2010; 290: 148-156.
  • Taussig SJ, Szekerczes J, Batkin S. Inhibition of tumour growth in vitro by bromelain, an extract of the pineapple plant (Ananas comosus). Planta Medica. 1985; 6: 538-539.
  • Amini A, Ehteda A, Masoumi S. Cytotoxic effects of bromelain in human gastrointestinal carcinoma cell lines (MKN45, KATO-III, HT29-5F12 and HT29-5M21). Onco Targets Ther. 2013; 6: 403–409.
  • Tysnes BB, Maurer HR, Porwol T, Probst B, Bjerkvig R, Hoover F. Bromelain Reversibly Inhibits Invasive Properties of Glioma Cells. Neoplasia. 2001; 3: 469–479.
  • Béez R, Lopes MTP, Salas CE, Hernández M. In vivo antitumoral activity of stem pineapple (Ananas comosus) bromelain. Planta Medica. 2007; 73: 1377-1383.
  • Mantovani A, Allavena P, Sica A, Balkwill F. Cancer-related inflammation. Nature. 2008; 454: 436-444.
  • Ferris RL, Grandis JR. NF-κB gene signatures and p53 mutations in head and neck squamous cell carcinoma. Clin Cancer Res. 2007; 13: 5663–5664.
  • Huang JR, Wu CC, Hou RCW, Jeng KC. Bromelain inhibits lipopolysaccharide-induced cytokine production in human THP-1 monocytes via the removal of CD14. Immunol Invest. 2008; 37: 263–277.
  • Hou RCW, Chen YS, Huang JR, Jeng KCG. Cross-linked bromelain inhibits lipopolysaccharide-induced cytokine production involving cellular signaling suppression in rats. J Agricultural Food Chemistry. 2006; 54:2193-2198.
  • Chang TC, Wei PL, Makondi PT, Chen WT, Huang CY , Chang YJ. Bromelain inhibits the ability of colorectal cancer cells to proliferate via activation of ROS production and autophagy. PLOS ONE. 2019; 14: e210274.
  • Raeisi F, Shahbazi-Gahrouei D, Raeisi E, Heidarian S. Evaluation of the Radiosensitizing Potency of Bromelain for Radiation Therapy of 4T1 Breast Cancer Cells. J Med Signals Sens. 2019; 9: 68–74.
  • Engwerda CR, Andrew D, Ladhams A, Mynott TL. Bromelain modulates T cell and B cell immune responses in vitro and in vivo. Cell Immunol. 2001; 210: 66-75.
  • Engwerda CR, Andrewa D, Murphya M, Mynottb TL. Bromelain activates murine macrophages and natural killer cells in vitro. Cell Immunol. 2001; 210: 5-10.
  • Fouz N, Amid A, Hashim Z. Gene Expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain. Appl Biochem Biotechnol. 2014; 173: 1618–1639.
  • Pillai K, Akhter J, Chua T, Morris D. Anticancer property of bromelain with therapeutic potential in malignant peritoneal mesothelioma. Cancer Invest. 2013; 31: 241-250.
  • Gani M, Nasiri R, Hamzehalipour J. In vitro antiproliferative activity of fresh pineapple juices on ovarian and colon cancer cell lines. Int J Pept Res Ther. 2015; 21: 353–364.
  • Mekkawy MH, Fahmy HA, Nada AS, Ali OS. Study of the radiosensitizing and radioprotective efficacy of bromelain (a pineapple extract): in vitro and in vivo. Integr Cancer Ther. 2020; 19: 1-14.
  • Chermahini FA, Raeisi E, Aazami MH, Mirzaei A, Heidarian E, Lemoigne Y. Does bromelain-cisplatin combination afford in vitro synergistic anticancer effects on human prostatic carcinoma cell line, PC3? Galen Med J. 2020; 9. 2588-2767.
  • Murthy SS, Narsaiah TB. Cytotoxic Effect of bromelain on HepG2 hepatocellular carcinoma cell line. Appl Biochem Biotechnol. 2021; 193: 1873-1897.
  • Mekkawy AH, Pillai K, Badar S, Akhter J, Ke K, Valle SJ, et al. Addition of bromelain and acetylcysteine to gemcitabine potentiates tumor inhibition in vivo in human colon cancer cell line LS174T. Am J Cancer Res. 2021; 11: 2252–2263.
  • Easty DM, Easty GC, Carter RL, Monaghan P, Butler LJ. Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck. Br J Cancer. 1981; 43: 772–785.
  • Pauzi AZ, Yeap SK, Abu N, Lim KL, Omar AR, Aziz SA, et al. Combination of cisplatin and bromelain exerts synergistic cytotoxic effects against breast cancer cell line MDA-MB-231 in vitro. Chin Med. 2016; 11: 46.
  • Manosroi W, Chankhampan C, Aranya J. In vitro Anti-cancer activity comparison of the freeze-dried and spray dried bromelain from pineapple stems. Chiang Mai J Sci. 2017; 44: 1407-1418.
  • Mohamad NE, Abu N, Yeap SK, Alitheen NB. Bromelain enhances the anti-tumor effects of cisplatin on 4T1 bre-ast tumor model in vivo. Integr Cancer Ther. 2019;18:1-7.
  • Lah T, Alonso M, Noorden C. Antiprotease therapy in cancer: hot or not? Expert Opin on Biol Ther. 2006; 6: 257-279.
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