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Abdelaziz, Lobna, Harb, Ola, Abdelbary, Abeer, Mohammed, Amrallah, Elkalla, Hend. (1401). The Prognostic Significance of ALDH-1 and SOX9 Expression in Early Breast Cancer. سامانه مدیریت نشریات علمی, 13(4), 581-592. doi: 10.30476/mejc.2022.90510.1578
Lobna A. Abdelaziz; Ola A. Harb; Abeer M. Abdelbary; Amrallah A. Mohammed; Hend M. Hamdey Rashed Elkalla. "The Prognostic Significance of ALDH-1 and SOX9 Expression in Early Breast Cancer". سامانه مدیریت نشریات علمی, 13, 4, 1401, 581-592. doi: 10.30476/mejc.2022.90510.1578
Abdelaziz, Lobna, Harb, Ola, Abdelbary, Abeer, Mohammed, Amrallah, Elkalla, Hend. (1401). 'The Prognostic Significance of ALDH-1 and SOX9 Expression in Early Breast Cancer', سامانه مدیریت نشریات علمی, 13(4), pp. 581-592. doi: 10.30476/mejc.2022.90510.1578
Abdelaziz, Lobna, Harb, Ola, Abdelbary, Abeer, Mohammed, Amrallah, Elkalla, Hend. The Prognostic Significance of ALDH-1 and SOX9 Expression in Early Breast Cancer. سامانه مدیریت نشریات علمی, 1401; 13(4): 581-592. doi: 10.30476/mejc.2022.90510.1578

The Prognostic Significance of ALDH-1 and SOX9 Expression in Early Breast Cancer

Middle East Journal of Cancer
مقاله 3، دوره 13، شماره 4 - شماره پیاپی 52، دی 2022، صفحه 581-592    اصل مقاله (2.98 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2022.90510.1578
نویسندگان
Lobna A. Abdelaziz* 1؛ Ola A. Harb2؛ Abeer M. Abdelbary2؛ Amrallah A. Mohammed3؛ Hend M. Hamdey Rashed Elkalla4
1Department of Clinical Oncology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
2Department of Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
3Department of Medical Oncology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
4Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Mansoura University, Mansoura, Egypt
چکیده
Background: Aldehyde dehydrogenase 1 (ALDH1) is an enzyme accountable for the detoxification of aldehydes. Sex-determining region Y-box 9 (SOX-9) plays a role in many biological and pathological processes. In this study, we aimed to evaluate the prognostic significance of ALDH1 and SOX9 expression in early breast cancer.
Method: The expression of ALDH1 and SOX-9 was evaluated through immunohistochemistry derived from 50 eligible patients with early breast cancer included in the current prospective cohort study.
Results: Positive expression of ALDH1 and SOX-9 were detected in 29 (58%) and 34 (68%) patients, respectively. The positive expressions of both markers were statistically significant associated with increasing the stage, lymph nodes metastasis, high Ki67 labeling index, and molecular subtypes (P < 0.001), along with with the biological markers; estrogen receptors, progesterone receptors, and human epidermal growth factor receptor 2 over-expressions, and large tumor size (P = 0.039, P = 0.022, P = 0.024 and P = 0.003 for ALDH1 expression and P = 0.012, P = 0.007, P = 0.004 , and P = 0.002 for SOX-9 expression, respectively). There is a significant positive association between the expression of ALDH1 and SOX-9, r (correlation coefficient) = +0.806 (P < 0.001). Local recurrence was associated with the positive expression of ALDH1 only (P = 0.045) and the disease progression was statistically significant and associated with the positive expression of both ALDH1 and SOX-9 (P = 0.038, P = 0.023, respectively). There was significant association of positive expression of SOX-9 with reduced 3-y disease-free survival (P = 0.039).
Conclusion: Positive expression of ALDH-1 and SOX9 were associated with aggressive histopathological features and poor outcome in early breast cancer and can be considered potential prognostic markers in this group of patients.
کلیدواژه‌ها
Early breast cancer؛ Neoplastic stem cells؛ Aldehyde dehydrogenase 1؛ SOX9؛ Prognosis
اصل مقاله

This article has been partially presented as an E-poster (184P) in ESMO virtual congress 2020 and published with a citation in Annals of Oncology (2020) 31 (suppl_4): S303-S339. doi:10.1016/annonc/annonc267.

How to cite this article:

Abdelaziz LA, Harb OA, Abdelbary AM, Mohammed AA, Hamdey Rashed Elkalla HM. The prognostic significance of ALDH-1 and SOX9 expression in early breast cancer. Middle East J Cancer. 2022;13(4):581-92. doi: 10.30476/mejc.2022.90510.1578.

مراجع
1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2017. CA Cancer J Clin. 2017;67(1):7-30. doi: 10.3322/caac.21387.
2. Miyoshi Y, Shien T, Ogiya A, Ishida N, Yamazaki K, Horii R, et al. Differences in expression of the cancer stem cell marker aldehyde dehydrogenase 1 among estrogen receptor-positive/human epidermal growth factor receptor type 2-negative breast cancer cases with early, late, and no recurrence. Breast Cancer Res. 2016;18(1):73. doi: 10.1186/s13058-016-0731-3.
3. Lapidot T, Sirard C, Vormoor J, Murdoch B, Hoang T, Caceres-Cortes J, et al. A cell initiating human acute myeloid leukaemia after transplantation into SCID mice. Nature. 1994;367(6464):645-8. doi: 10.1038/ 367645a0.
4. Zhong Y, Shen S, Zhou Y, Mao F, Guan J, Lin Y, et al. ALDH1 is a better clinical indicator for relapse of invasive ductal breast cancer than the CD44+/CD24- phenotype. Med Oncol. 2014;31(3):864. doi: 10.1007/ s12032-014-0864-0
5. Althobiti M, El Ansari R, Aleskandarany M, Joseph C, Toss MS, Green AR, et al. The prognostic significance of ALDH1A1 expression in early invasive breast cancer. Histopathology. 2020;77(3):437-48. doi: 10.1111/his.14129.
6.Vassalli G. Aldehyde dehydrogenases: not just markers, but functional regulators of stem cells. Stem Cells Int. 2019; 3904645. doi.org/10.1155/2019/3904645.
7. Ciccone V, Terzuoli E, Donnini S, Giachetti A, Morbidelli L, Ziche M. Correction to: Stemness marker aldh1a1 promotes tumor angiogenesis via retinoic acid/hif-1alpha/vegf signalling in mcf-7 breast cancer cells. J Exp Clin Cancer Res. 2019;38;45. doi:10.1186/ s13046-019-1045-y.
8. Hersmus R, Kalfa N, de Leeuw B, Stoop H, Oosterhuis JW, de Krijger R, et al. FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD). J Pathol. 2008; 215: 31-8. doi:10.1002/path.2335.
9. Ruan H, Hu S, Zhang H, Du G, Li X, Li X, et al. Upregulated SOX9 expression indicates worse prognosis in solid tumors: a systematic review and meta-analysis. Oncotarget. 2017;8(68):113163-73. doi: 10.18632/oncotarget.22635.
10. Higo N, Okumura H, Uchikado Y, Omoto I, Sasaki K, Kita Y, et al. Expression of SOX9 is related to prognosis in patients with oesophageal squamous cell carcinoma. In Vivo. 2018;32:835-8. doi: 10.21873/in vivo. 112316.
11. Edge SB, Compton CC. The American Joint Committee on Cancer: the 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 2010;17(6):1471-4. doi: 10.1245/s10434-010-0985-4.
12. Dimitrakopoulos FI, Kottorou A, Antonacopoulou AG, Makatsoris T, Kalofonos HP. Early-stage breast cancer in the elderly: Confronting an old clinical problem. J Breast Cancer. 2015;18(3):207-17. doi: 10.4048/jbc.2015.18.3.207.
13. Hammond ME, Hayes DF, Dowsett M, Allred DC, Hagerty KL, Badve S, et al. American Society Of Clinical Oncology/College of American Pathologists guideline recommendations for IHC testing of estrogen and progesterone receptors in breast cancer. Arch Pathol Lab Med. 2010;134:907-22. doi: 10.5858/ 134.7.e48.
14. Gutierrez C, Schiff R. HER2 biology, detection, and clinical implications. Arch Pathol Lab Med. 2011;135:55-62. doi: 10.5858/2010-0454-RAR.1.
15. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981;29:577-80. doi: 10.1177/29.4.6166661.
16. Tingting Li, Huang H, Shi G, Zhao L, Li T, Zhang Z, et al. TGF-β1-SOX9 axis-inducible COL10A1 promotes invasion and metastasis in gastric cancer via epithelial-tomesenchymal transition. Cell Death and Disease. 2018;9:849. doi:10.1038/s41419-018-0877-2.
17. Lei B, Zhang Y, Liu T, Li Y, Pang D. Sox9 upregulation in breast cancer is correlated with poor prognosis and the CD44+/CD24-/low phenotype. Int J Clin Exp Pathol. 2016; 9(7):7345-51. doi:10.31557/APJCP.2 020.21.9.2561.
18. Grantzdorffer I, Yumlu S, Gioeva Z, Von Wasielewski R, Ebert MP, Röcken C. Comparison of different tissue sampling methods for protein extraction from formalin-fixed and paraffin-embedded tissue specimens. Exp Mol Pathol. 2010;88(1):190-6. doi: 10.1016/j. yexmp.2009.09.009.
19. Wang Y, Zhe H, Gao P, Zhang N, Li G, Qin J. Cancer stem cell marker ALDH1 expression is associated with lymph node metastasis and poor survival in esophageal squamous cell carcinoma. a study from high incidence area of northern China. Dis Esophagus. 2012;25(6):560-5. doi: 10.1111/j.1442-2050.2011. 01279.
20. Liu Y, Lv DL, Duan JJ, Xu SL, Zhang JF, Yang XJ, et al. ALDH1A1 expression correlates with clinicopathologic features and poor prognosis of breast cancer patients: a systematic review and meta-analysis. BMC Cancer. 2014;14:444. doi: 10.1186/1471-2407-14-444.
21. Kadaja M, Keyes BE, Lin M, Pasolli H, Genander M, Polak L, et al. SOX9 a stem cell transcriptional regulator of secreted niche signaling factors. Genes Dev. 2014;28:328-41. doi: 10.1101/gad.233247.113.
22. Larsimont JC, Youssef KK, Sánchez-Danés A, Sukumaran V, Defrance M, Delatte B, et al. SOX9 controls self-renewal of oncogene targeted cells and links tumor initiation and invasion. Cell Stem Cell. 2015;17:60-73. doi: 10.1016/j.stem.2015.05.008.
23. Morimoto K, Kim SJ, Tanei T, Shimazu K, Tanji Y, Taguchi T, et al. Stem cell marker aldehyde dehydrogenase 1-positive breast cancers are characterized by negative estrogen receptor, positive human epidermal growth factor receptor type 2, and high Ki67 expression. Cancer Sci. 2009;100(6):1062-8. doi: 10.1111/j.1349-7006.2009.01151.
24. Pan H, Wu N, Huang Y, Li Q, Liu C, Liang M, et al. Aldehyde dehydrogenase 1 expression correlates with the invasion of breast cancer. Diagn Pathol. 2015;10:66. doi: 10.1186/s13000-015-0301-5.
25. Chakravarty G, Moroz K, Makridakis N, Lloyd SA, Galvez SE, Canavello PR, et al. Prognostic significance of cytoplasmic SOX9 in invasive ductal carcinoma and metastatic breast cancer. Exp Biol Med (Maywood). 2011; 236:145. doi: 10.1258/ebm.2010.010086.
26. Strati A, Nikolaou M, Georgoulias V, Lianidou ES. Prognostic significance of TWIST1, CD24, CD44, and ALDH1 transcript quantification in EpCAM-positive circulating tumor cells from early stage breast cancer patients. Cells. 2019;8(7):652. doi: 10.3390/cells8070652.
27. Sarkar P, Basu K, Sarkar P, Chatterjee U, Mukhopadhyay M, Choudhuri M, et al. Correlations of aldehyde dehydrogenase 1(ALDH1) expression with traditional prognostic parameters and different molecular subtypes of breast carcinoma. Clujul Med. 2018;91(2):181-7. doi: 10.15386/cjmed-925.
28. Demir H, Dulgar O, Gulle BT, Turna H, Ilvan S. Prognostic value of aldehyde dehydrogenase 1 (ALDH1) in invasive breast carcinomas. Bosn J Basic Med Sci. 2019;7;18:313-9. doi: 10.17305/bjbms. 2018.3094.
29. Domenici G, Aurrekoetxea-Rodríguez I, Simões BM, Rábano M, Lee SY, Millán JS, et al. A Sox2Sox9 signalling axis maintains human breast luminal progenitor and breast cancer stem cells. Oncogene. 2019;38(17):3151-69. doi: 10.15386/cjmed-925.
30. Grimm D, Bauer J, Wise P, Krüger M, Simonsen U, Wehland M, et al. The role of SOX family members in solid tumours and metastasis. Semin Cancer Biol. 2020;67(Pt 1):122-53. doi: 10.1016/j.semcancer. 2019.03.004.
31. Ginestier C, Hur MH, Charafe-Jauffret E, Monville F, Dutcher J, Brown M, et al. ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell. 2007;1(5):555-67. doi: 10.1016/j.stem.2007. 08.014.
32. Lawson DA, Bhakta NR, Kessenbrock K, Prummel KD, Yu Y, Takai K, et al. Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells. Nature. 2015;526(7571):131-5. doi.org/10.1038/ nature15260.
33. Dong Y, Bi LR, Xu N, Yang HM, Zhang HT, Ding Y, et al. The expression of aldehyde dehydrogenase 1 in invasive primary breast tumors and axillary lymph node metastases is associated with poor clinical prognosis. Pathol Res Pract. 2013;209(9):555-61. doi: 10.1016/j.prp.2013.05.007.
34. Lapierre M, Linares A, Dalvai M, Duraffourd C, Bonnet S, Boulahtouf A, et al. Histone deacetylase 9 regulates breast cancer cell proliferation and the response to histone deacetylase inhibitors. Oncotarget. 2016;7:19693-708. doi: 10.18632/oncotarget.7564.
35. Riemenschnitter C, Teleki I, Tischler V, Guo W, Varga Z. Stability and prognostic value of Slug, Sox9 and Sox10 expression in breast cancers treated with neoadjuvant chemotherapy. Springerplus. 2013;2:695. doi: 10.1186/2193-1801-2-695.
36. Willis S, De P, Dey N, Long B, Young B, Sparano JA, et al. Enriched transcription factor signatures in triple negative breast cancer indicates possible targeted therapies with existing drugs. Meta Gene. 2015;4:129-41. doi: 10.1016/j.mgene.2015.04.002.
37. Chatterjee D, Bal A, Das A, Singh G. Proliferation rate and breast cancer subtype, but not ALDH1 expression, predict pathological response to neoadjuvant chemotherapy in locally advanced breast cancer. Virchows Arch. 2015;467(3):303-10. doi: 10.1007/s00428-015-1794-8.
38. Ito M, Shien T, Omori M, Mizoo T, Iwamoto T, Nogami T, et al. Evaluation of aldehyde dehydrogenase 1 and transcription factors in both primary breast cancer and axillary lymph node metastases as a prognostic factor. Breast Cancer. 2016; 23(3):437-44. doi: 10.1007/ s12282-015-0583-1.
39. Resetkova E, Reis-Filho JS, Jain RK, Mehta R, Thorat MA, Nakshatri H, et al. Prognostic impact of ALDH1 in breast cancer: a story of stem cells and tumor microenvironment. Breast Cancer Res Treat. 2010; 123(1):97-108. doi: 10.1007/s10549-009-0619-3.
40. Yoshioka T, Umekita Y, Ohi Y, Souda M, Sagara Y, Sagara Y, et al. Aldehyde dehydrogenase 1 expression is a predictor of poor prognosis in node-positive breast cancers: a long-term follow-up study. Histopathology. 2011;58(4):608-16. doi: 10.1186/1471-2407-14-444.

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