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Mahdizade Valojerdi, Fereshte, Goliaei, Bahram, Rezakhani, Nakisa, Nikoofar, Alireza, Keshmiri Neghab, Hoda, Soheilifar, Mohammad Hasan, Bigdeli, Bahareh. (1402). In Vitro Radiosensitization of T47D and MDA-MB-231 Breast Cancer Cells with the Neoflavonoid Dalbergin. سامانه مدیریت نشریات علمی, 14(2), 205-218. doi: 10.30476/mejc.2022.91913.1637
Fereshte Mahdizade Valojerdi; Bahram Goliaei; Nakisa Rezakhani; Alireza Nikoofar; Hoda Keshmiri Neghab; Mohammad Hasan Soheilifar; Bahareh Bigdeli. "In Vitro Radiosensitization of T47D and MDA-MB-231 Breast Cancer Cells with the Neoflavonoid Dalbergin". سامانه مدیریت نشریات علمی, 14, 2, 1402, 205-218. doi: 10.30476/mejc.2022.91913.1637
Mahdizade Valojerdi, Fereshte, Goliaei, Bahram, Rezakhani, Nakisa, Nikoofar, Alireza, Keshmiri Neghab, Hoda, Soheilifar, Mohammad Hasan, Bigdeli, Bahareh. (1402). 'In Vitro Radiosensitization of T47D and MDA-MB-231 Breast Cancer Cells with the Neoflavonoid Dalbergin', سامانه مدیریت نشریات علمی, 14(2), pp. 205-218. doi: 10.30476/mejc.2022.91913.1637
Mahdizade Valojerdi, Fereshte, Goliaei, Bahram, Rezakhani, Nakisa, Nikoofar, Alireza, Keshmiri Neghab, Hoda, Soheilifar, Mohammad Hasan, Bigdeli, Bahareh. In Vitro Radiosensitization of T47D and MDA-MB-231 Breast Cancer Cells with the Neoflavonoid Dalbergin. سامانه مدیریت نشریات علمی, 1402; 14(2): 205-218. doi: 10.30476/mejc.2022.91913.1637

In Vitro Radiosensitization of T47D and MDA-MB-231 Breast Cancer Cells with the Neoflavonoid Dalbergin

Middle East Journal of Cancer
مقاله 2، دوره 14، شماره 2 - شماره پیاپی 54، تیر 2023، صفحه 205-218    اصل مقاله (3.84 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2022.91913.1637
نویسندگان
Fereshte Mahdizade Valojerdi* 1؛ Bahram Goliaei2؛ Nakisa Rezakhani1؛ Alireza Nikoofar3؛ Hoda Keshmiri Neghab4؛ Mohammad Hasan Soheilifar5؛ Bahareh Bigdeli2
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
3Department of Radiotherapy, Iran University of Medical Sciences (IUMS), Tehran, Iran
4Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
5Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
چکیده
Background: Radiotherapy is a frequently used therapeutic modality for breast cancer. Dalbergin, a natural antioxidant, inhibits carcinogens and tumor progression. In the present study, we investigated the effect of Dalbergin on the response of T47D and MDA-MB-231 breast cancer cell lines to ionizing radiation.
Method: In this experimental in vitro study, doubling time of T47D and MDAMB- 231 were obtained from the growth curve. The cytotoxic effect of Dalbergin on T47D and MDA-MB-231 breast cancer cells were estimated via MTT assay. To determine the clonogenic ability, we treated T47D and MDA-MB-231 with Dalbergin for 48 h prior to irradiation, subsequent to which a colony assay was performed. Real-time polymerase chain reaction was employed to determine the gene expression level.
Results: Dalbergin inhibited proliferation of T47D and MDA-MB-231 in a time and concentration-dependent manner. Additionally, the most appropriate time for the treatment of these types of cancer cells was found to be 48 h and the drug's concentration in both cell lines was different. The IC50 values of T47D and MDA-MB-231 cells were 0.001 and 0.0001 μM, respectively. Moreover, this drug radiaosensitizes both cell lines effectively compared with the radiation only. Finally, the gene expression level of p53, Bcl-2, and STAT3 were investigated in cancer cells.
Conclusion: Dalbergin showed apoptotic effects probably through the STAT/p53 signaling pathway. Therefore, Dalbergin could be considered as a radiosensitizer and its effects may be owing to increased cell death.
کلیدواژه‌ها
Dalbergin؛ Cells؛ X-rays؛ Apoptotic؛ Cell death
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