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Hamzian, Nima, Nickfarjam, Abolfazl, Shams, Ali, Haghiralsadat, Fateme, Najmi-Nezhad, Moslem. (1401). Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect. سامانه مدیریت نشریات علمی, 12(2), 137-148. doi: 10.31661/jbpe.v0i0.2109-1397
Nima Hamzian; Abolfazl Nickfarjam; Ali Shams; Fateme Haghiralsadat; Moslem Najmi-Nezhad. "Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect". سامانه مدیریت نشریات علمی, 12, 2, 1401, 137-148. doi: 10.31661/jbpe.v0i0.2109-1397
Hamzian, Nima, Nickfarjam, Abolfazl, Shams, Ali, Haghiralsadat, Fateme, Najmi-Nezhad, Moslem. (1401). 'Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect', سامانه مدیریت نشریات علمی, 12(2), pp. 137-148. doi: 10.31661/jbpe.v0i0.2109-1397
Hamzian, Nima, Nickfarjam, Abolfazl, Shams, Ali, Haghiralsadat, Fateme, Najmi-Nezhad, Moslem. Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect. سامانه مدیریت نشریات علمی, 1401; 12(2): 137-148. doi: 10.31661/jbpe.v0i0.2109-1397

Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect

Journal of Biomedical Physics and Engineering
مقاله 5، دوره 12، شماره 2، تیر 2022، صفحه 137-148    اصل مقاله (8.14 M)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.2109-1397
نویسندگان
Nima Hamzian1؛ Abolfazl Nickfarjam1؛ Ali Shams2؛ Fateme Haghiralsadat3؛ Moslem Najmi-Nezhad* 4، 5
1PhD, Department of Medical Physics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2PhD, Department of Immunology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3PhD, Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4MSc, Department of Medical Physics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
5MSc, Department of Biochemistry and Biophysics, School of Paramedicine, Iranshahr University of Medical Sciences, Iranshahr, Iran
چکیده
Background: The Mentha-Pulegium essential oil (MP-EO) contains different antioxidant compounds and reduces the indirect effects of dispersed ionizing radiation on biological systems.
Objective: The current study aimed to assess a possible radio-protective effect of MP-EO on peripheral blood mononuclear cells (PBMCs).
Material and Methods: In this experimental study, MP-EO was firstly prepared and PBMCs were then irradiated in various groups with doses of 25 and 200 cGy of X-rays in the presence of IC10 of MP-EO. After incubation times of 48h and 72h, the survival, apoptosis, and necrosis percentages of PBMCs were determined by MTT assay and flow cytometry analyses; the radio-protective effect of MP-EO was examined.
Results: In the presence of 80 µg/ml (IC10) MP-EO, the mean survival percentage of irradiated PBMCs by radiation doses of 25 and 200 cGy was significantly increased after 48h of incubation compared with the control. At 72h of incubation, the mean survival percentage of irradiated PBMCs was significantly increased only at 25 cGy. The percentage of apoptosis and necrosis of PBMCs was significantly reduced in the presence of the MP-EO at both incubation times and radiation doses; therefore, the highest reduction was at 200 cGy and 48h incubation compared to the control.
Conclusion: MP-EO as a natural, non-toxic, and cost-effective compound can exhibit a favorable in-vitro radio-protective effect by increasing the survival and decreasing the percentage of apoptosis and necrosis of irradiated PBMCs.
کلیدواژه‌ها
Lymphocytes؛ Mentha Pulegium Essential Oil؛ Radiation, Ionizing؛ Radioprotective؛ Apoptosis؛ Necrosis
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