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Najafzadeh, Faezeh, Tanomand, Asghar, Hadadi, Azam, Majidi, Jafar. (1400). Immunological Properties of Exotoxin A Toxoid - Detoxified Lipopolysaccharide - Gold Nanoparticles Conjugate Against Pseudomonas aeruginosa Infection. سامانه مدیریت نشریات علمی, 18(4), 292-303. doi: 10.22034/iji.2021.87816.1832
Faezeh Najafzadeh; Asghar Tanomand; Azam Hadadi; Jafar Majidi. "Immunological Properties of Exotoxin A Toxoid - Detoxified Lipopolysaccharide - Gold Nanoparticles Conjugate Against Pseudomonas aeruginosa Infection". سامانه مدیریت نشریات علمی, 18, 4, 1400, 292-303. doi: 10.22034/iji.2021.87816.1832
Najafzadeh, Faezeh, Tanomand, Asghar, Hadadi, Azam, Majidi, Jafar. (1400). 'Immunological Properties of Exotoxin A Toxoid - Detoxified Lipopolysaccharide - Gold Nanoparticles Conjugate Against Pseudomonas aeruginosa Infection', سامانه مدیریت نشریات علمی, 18(4), pp. 292-303. doi: 10.22034/iji.2021.87816.1832
Najafzadeh, Faezeh, Tanomand, Asghar, Hadadi, Azam, Majidi, Jafar. Immunological Properties of Exotoxin A Toxoid - Detoxified Lipopolysaccharide - Gold Nanoparticles Conjugate Against Pseudomonas aeruginosa Infection. سامانه مدیریت نشریات علمی, 1400; 18(4): 292-303. doi: 10.22034/iji.2021.87816.1832

Immunological Properties of Exotoxin A Toxoid - Detoxified Lipopolysaccharide - Gold Nanoparticles Conjugate Against Pseudomonas aeruginosa Infection

Iranian Journal of Immunology
دوره 18، شماره 4، اسفند 2021، صفحه 292-303    اصل مقاله (1.24 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2021.87816.1832
نویسندگان
Faezeh Najafzadeh1؛ Asghar Tanomand* 2؛ Azam Hadadi1؛ Jafar Majidi3
1Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
2Departement of Microbiology, Maragheh University of Medical sciences, Maragheh, Iran.
3Department of Immunology and Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
چکیده
Background: Pseudomonas aeruginosa is an important opportunistic pathogen, especially in patients with compromised host defense. Objective: To prepare the conjugate of detoxified lipopolysaccharide (D-LPS) and exotoxin A toxoid (T-ETA) from P. aeruginosa in gold nanoparticles (Au NPs) in a mice model. Methods: LPS and ETA were purified from P. aeruginosa PAO1. D-LPS was conjugated with T-ETA via the amidation method. Au NPs were bound to D-LPS-T-ETA conjugate via electrostatic interaction. Mice were immunized with D-LPS, D-LPS-Au NPs, T-ETA, T-ETA-Au NPs, D-LPS-T-ETA, D-LPS-T-ETA-Au NPs, D-LPS-Au NPs+T-ETA-Au NPs, Au NPs, and phosphate-buffered saline (PBS), and specific IgG titers were determined by the ELISA and the whole-cell ELISA methods. Mice in the vaccinated and control groups were exposed to a 2×LD50 of P. aeruginosa and mortality rates were recorded for one week. Results: The results showed that vaccination by D-LPS, D-LPS-Au NPs, T-ETA, T-ETA-Au NPs, D-LPS-T-ETA, D-LPS-T-ETA-Au NPs and D-LPS-Au NPs+T-ETA-Au NPs induced specific IgG. Mice received the D-LPS-T-ETA-Au NPs conjugate showed significant protection against bacterial challenge. Conclusion: These data indicate that D-LPS-T-ETA-Au NPs conjugate has a significant immunogenicity potential to be applied as a new vaccine against Pseudomonas infections.
کلیدواژه‌ها
Exotoxin A (ETA)؛ Gold Nanoparticles (Au NPs)؛ Lipopolysaccharide (LPS)؛ Pseudomonas aeruginosa؛ Vaccine Candidate
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