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Peng, Yunzhi, Bai, Wenhui, Wang, Zhanli, Yu, Hui. (1400). TLR9/NF-kB Pathway Regulates Brucella CpG DNA-mediated Cytokine Response in Human Peripheral Blood Mononuclear Cells. سامانه مدیریت نشریات علمی, 18(4), 268-278. doi: 10.22034/iji.2021.84578.1665
Yunzhi Peng; Wenhui Bai; Zhanli Wang; Hui Yu. "TLR9/NF-kB Pathway Regulates Brucella CpG DNA-mediated Cytokine Response in Human Peripheral Blood Mononuclear Cells". سامانه مدیریت نشریات علمی, 18, 4, 1400, 268-278. doi: 10.22034/iji.2021.84578.1665
Peng, Yunzhi, Bai, Wenhui, Wang, Zhanli, Yu, Hui. (1400). 'TLR9/NF-kB Pathway Regulates Brucella CpG DNA-mediated Cytokine Response in Human Peripheral Blood Mononuclear Cells', سامانه مدیریت نشریات علمی, 18(4), pp. 268-278. doi: 10.22034/iji.2021.84578.1665
Peng, Yunzhi, Bai, Wenhui, Wang, Zhanli, Yu, Hui. TLR9/NF-kB Pathway Regulates Brucella CpG DNA-mediated Cytokine Response in Human Peripheral Blood Mononuclear Cells. سامانه مدیریت نشریات علمی, 1400; 18(4): 268-278. doi: 10.22034/iji.2021.84578.1665

TLR9/NF-kB Pathway Regulates Brucella CpG DNA-mediated Cytokine Response in Human Peripheral Blood Mononuclear Cells

Iranian Journal of Immunology
دوره 18، شماره 4، اسفند 2021، صفحه 268-278    اصل مقاله (2.72 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2021.84578.1665
نویسندگان
Yunzhi Peng1؛ Wenhui Bai1؛ Zhanli Wang* 1؛ Hui Yu1، 2
1Inner Mongolia Key Laboratory of Disease-Related Biomarkers, Baotou Medical College, Baotou 014060, China.
2School of Basic Medicine, Baotou Medical College, Baotou 014060, China.
چکیده
Background: It was reported that targeting the Toll-like receptor9(TLR9) signaling pathway can be a promising therapeuticstrategy forinterventions in various inflammatory and infectiousdiseases. However,it was not known whether the human TLR9 isresponsive to Brucellacytidine-phosphate-guanosine (CpG) DNAsequences and activatesthehost’s innate immune system. Objective: The present study aimed to identify the novel humanTLR9agonists from Brucella CpG oligodeoxynucleotide(ODN) candidatesand verify their immune response regulatorymechanisms. Methods: Molecular docking methods were used to discover potentagonists of the human TLR9. The potential molecules were furthervalidated by Western blot and enzyme-linked immunosorbentassay(ELISA). Results: The experiment results showed a strong interactionandgood compatibility between the human TLR9 and BrucellaODN-1molecule. In addition, the induction of immune response byBrucella ODN-1 is a CpG-specific response. Moreover, the effectsof Brucella ODN-1 on cytokine response are dependent on theTLR9-mediated NF-κB pathway. Conclusion: These results indicated that the Brucella ODN-1 molecule canserve as a starting point to discover or designmore potent and specific TLR9 agonists that have the potential usein the treatment of infectious diseases.
کلیدواژه‌ها
Agonist؛ Brucella؛ Cytidine-phosphateguanosine (CpG) motif؛ Toll-like receptor 9
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