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Morshedi, Gholamreza, Ghanbarinejad, Vahid, Zareei, Mina, Poornajaf, Abazar, Hassanian-Moghaddam, Hossein, Hadi, Vahid, Isvand, Abbas, Saadati, Mohammad reza, Maleki, Homayoun, Keshavarzi, Majid. (1400). PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection. سامانه مدیریت نشریات علمی, 8(1), 51-56. doi: 10.30476/tips.2021.93218.1121
Gholamreza Morshedi; Vahid Ghanbarinejad; Mina Zareei; Abazar Poornajaf; Hossein Hassanian-Moghaddam; Vahid Hadi; Abbas Isvand; Mohammad reza Saadati; Homayoun Maleki; Majid Keshavarzi. "PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection". سامانه مدیریت نشریات علمی, 8, 1, 1400, 51-56. doi: 10.30476/tips.2021.93218.1121
Morshedi, Gholamreza, Ghanbarinejad, Vahid, Zareei, Mina, Poornajaf, Abazar, Hassanian-Moghaddam, Hossein, Hadi, Vahid, Isvand, Abbas, Saadati, Mohammad reza, Maleki, Homayoun, Keshavarzi, Majid. (1400). 'PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection', سامانه مدیریت نشریات علمی, 8(1), pp. 51-56. doi: 10.30476/tips.2021.93218.1121
Morshedi, Gholamreza, Ghanbarinejad, Vahid, Zareei, Mina, Poornajaf, Abazar, Hassanian-Moghaddam, Hossein, Hadi, Vahid, Isvand, Abbas, Saadati, Mohammad reza, Maleki, Homayoun, Keshavarzi, Majid. PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection. سامانه مدیریت نشریات علمی, 1400; 8(1): 51-56. doi: 10.30476/tips.2021.93218.1121

PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection

Trends in Pharmaceutical Sciences
مقاله 7، دوره 8، شماره 1، خرداد 2022، صفحه 51-56    اصل مقاله (433.43 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/tips.2021.93218.1121
نویسندگان
Gholamreza Morshedi1؛ Vahid Ghanbarinejad2؛ Mina Zareei2؛ Abazar Poornajaf3؛ Hossein Hassanian-Moghaddam4، 5؛ Vahid Hadi6؛ Abbas Isvand7؛ Mohammad reza Saadati7؛ Homayoun Maleki8؛ Majid Keshavarzi* 9
1Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
2Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
4Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5Department of Clinical Toxicology, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
6Department of Biochemistry and Nutrition, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
7Department of Food Quality Control Laboratory, Army Health Center of Excellence (NEZAJA), Tehran, Iran
8Department of Education and Research, Army Health Center of Excellence (NEZAJA), Tehran, Iran
9Department of Environmental Health Engineering, School of Public Health, Sabzevar University of Medical Sciences, Sabzevar, Iran
چکیده
Exotoxin A (exoA) is a major pathogenic factor in Pseudomonas aeruginosa. This non-fermenting gram-negative bacillus causes a wide range of infections. P. aeruginosa is a major cause of hospital-acquired infections. With this view, this study was aimed to identify P. aeruginosa strains carrying the exoA gene isolated from burn wounds. As part of the cross-sectional study, 110 samples of burns were collected in several hospitals. After identification of bacterial strains by biochemical and microbiological tests, genomic DNA was extracted. PCR reaction was performed for screening for exoA gene. After examining DNA extracted using quantitative (Nano drop with OD260/280=1.8-2nm) and qualitative (electrophoresis on the 0.7% gel agarose) methods, 75.4% (n; 83 of 110) isolates carried the exoA gene. The high prevalence of the exoA gene in P. aeruginosa strains indicate the important role of this exoenzyme in the spread of infection in burn patients. Therefore, it is recommended that people with burn infections be screened for the exoA gene.
 
Please cite this article as: Gholamreza Morshedi, Vahid Ghanbarinejad, Mina Zarei, Abazar Pournajaf, Hossein
Hassanian-Moghaddam, Vahid Hadi,Abbas Isvand, Mohammad Reza Saadati, Homayoun Maleki, Majid Keshavarzi. PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection. Trends in Pharmaceutical Sciences. 2022;8(1):51-56 .doi: 10.30476/TIPS.2021.93218.1121
کلیدواژه‌ها
Exotoxin A؛ Pseudomonas aeruginosa؛ Burn Wound
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