LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,113
تعداد مقالات10,208
تعداد مشاهده مقاله40,780,297
تعداد دریافت فایل اصل مقاله8,804,022
Morshedi, Gholamreza, Ghanbarinejad, Vahid, Zareei, Mina, Poornajaf, Abazar, Hassanian-Moghaddam, Hossein, Hadi, Vahid, Isvand, Abbas, Saadati, Mohammad reza, Maleki, Homayoun, Keshavarzi, Majid. (1400). PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection. سامانه مدیریت نشریات علمی, 8(1), 51-56. doi: 10.30476/tips.2021.93218.1121
Gholamreza Morshedi; Vahid Ghanbarinejad; Mina Zareei; Abazar Poornajaf; Hossein Hassanian-Moghaddam; Vahid Hadi; Abbas Isvand; Mohammad reza Saadati; Homayoun Maleki; Majid Keshavarzi. "PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection". سامانه مدیریت نشریات علمی, 8, 1, 1400, 51-56. doi: 10.30476/tips.2021.93218.1121
Morshedi, Gholamreza, Ghanbarinejad, Vahid, Zareei, Mina, Poornajaf, Abazar, Hassanian-Moghaddam, Hossein, Hadi, Vahid, Isvand, Abbas, Saadati, Mohammad reza, Maleki, Homayoun, Keshavarzi, Majid. (1400). 'PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection', سامانه مدیریت نشریات علمی, 8(1), pp. 51-56. doi: 10.30476/tips.2021.93218.1121
Morshedi, Gholamreza, Ghanbarinejad, Vahid, Zareei, Mina, Poornajaf, Abazar, Hassanian-Moghaddam, Hossein, Hadi, Vahid, Isvand, Abbas, Saadati, Mohammad reza, Maleki, Homayoun, Keshavarzi, Majid. PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection. سامانه مدیریت نشریات علمی, 1400; 8(1): 51-56. doi: 10.30476/tips.2021.93218.1121

PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection

Trends in Pharmaceutical Sciences
مقاله 7، دوره 8، شماره 1، خرداد 2022، صفحه 51-56    اصل مقاله (433.43 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/tips.2021.93218.1121
نویسندگان
Gholamreza Morshedi1؛ Vahid Ghanbarinejad2؛ Mina Zareei2؛ Abazar Poornajaf3؛ Hossein Hassanian-Moghaddam4، 5؛ Vahid Hadi6؛ Abbas Isvand7؛ Mohammad reza Saadati7؛ Homayoun Maleki8؛ Majid Keshavarzi* 9
1Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
2Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
4Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5Department of Clinical Toxicology, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
6Department of Biochemistry and Nutrition, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
7Department of Food Quality Control Laboratory, Army Health Center of Excellence (NEZAJA), Tehran, Iran
8Department of Education and Research, Army Health Center of Excellence (NEZAJA), Tehran, Iran
9Department of Environmental Health Engineering, School of Public Health, Sabzevar University of Medical Sciences, Sabzevar, Iran
چکیده
Exotoxin A (exoA) is a major pathogenic factor in Pseudomonas aeruginosa. This non-fermenting gram-negative bacillus causes a wide range of infections. P. aeruginosa is a major cause of hospital-acquired infections. With this view, this study was aimed to identify P. aeruginosa strains carrying the exoA gene isolated from burn wounds. As part of the cross-sectional study, 110 samples of burns were collected in several hospitals. After identification of bacterial strains by biochemical and microbiological tests, genomic DNA was extracted. PCR reaction was performed for screening for exoA gene. After examining DNA extracted using quantitative (Nano drop with OD260/280=1.8-2nm) and qualitative (electrophoresis on the 0.7% gel agarose) methods, 75.4% (n; 83 of 110) isolates carried the exoA gene. The high prevalence of the exoA gene in P. aeruginosa strains indicate the important role of this exoenzyme in the spread of infection in burn patients. Therefore, it is recommended that people with burn infections be screened for the exoA gene.
 
Please cite this article as: Gholamreza Morshedi, Vahid Ghanbarinejad, Mina Zarei, Abazar Pournajaf, Hossein
Hassanian-Moghaddam, Vahid Hadi,Abbas Isvand, Mohammad Reza Saadati, Homayoun Maleki, Majid Keshavarzi. PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection. Trends in Pharmaceutical Sciences. 2022;8(1):51-56 .doi: 10.30476/TIPS.2021.93218.1121
کلیدواژه‌ها
Exotoxin A؛ Pseudomonas aeruginosa؛ Burn Wound
مراجع
1. Rumbaugh KP, Griswold JA, Hamood AN. Pseudomonas aeruginosa strains obtained from patients with tracheal, urinary tract and wound infection: variations in virulence factors and virulence genes. J Hosp Infect. 1999 Nov;43(3):211-8.
2. Sadikot RT, Blackwell TS, Christman JW, Prince AS. Pathogen-host interactions in Pseudomonas aeruginosa pneumonia. Am J Respir Crit Care Med. 2005 Jun 1;171(11):1209-23. doi: 10.1164/rccm.200408-1044SO.
3. O'Carroll MR, Syrmis MW, Wainwright CE, Greer RM, Mitchell P, Coulter C, Sloots TP, Nissen MD, Bell SC. Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units. Eur Respir J. 2004 Jul;24(1):101-6. doi: 10.1183/09031936.04.00122903.
4. Moore NM, Flaws ML. Epidemiology and pathogenesis of Pseudomonas aeruginosa infections. Clin Lab Sci. 2011 Winter;24(1):43-6. PMID: 21404964.
5. Jalili M, Ehrampoush MH, Zandi H, Ebrahimi AA, Mokhtari M, Samaei MR, Abbasi F. Risk assessment and disease burden of legionella presence in cooling towers of Iran's central hospitals. Environ Sci Pollut Res Int. 2021 Dec;28(46):65945-65951. doi: 10.1007/s11356-021-14791-9.
6. Sedlak-Weinstein E, Cripps AW, Kyd JM, Foxwell AR. Pseudomonas aeruginosa: the potential to immunise against infection. Expert Opin Biol Ther. 2005 Jul;5(7):967-82. doi: 10.1517/14712598.5.7.967. PMID: 16018741.
7. Grimwood K, Kyd JM, Owen SJ, Massa HM, Cripps AW. Vaccination against respiratory Pseudomonas aeruginosa infection. Hum Vaccin Immunother. 2015;11(1):14-20. doi: 10.4161/hv.34296. Epub 2014 Nov 1.
8. Faezi S, Safarloo M, Amirmozafari N, Nikokar I, Siadat SD, Holder IA, Mahdavi M. Protective efficacy of Pseudomonas aeruginosa type-A flagellin in the murine burn wound model of infection. APMIS. 2014 Feb;122(2):115-27.
9. Dong D, Zou D, Liu H, Yang Z, Huang S, Liu N, He X, Liu W, Huang L. Rapid detection of Pseudomonas aeruginosa targeting the toxA gene in intensive care unit patients from Beijing, China. Front Microbiol. 2015 Oct 6;6:1100. doi: 10.3389/fmicb.2015.01100.
10. Khosravi AD, Shafie F, Abbasi Montazeri E, Rostami S. The frequency of genes encoding exotoxin A and exoenzyme S in Pseudomonas aeruginosa strains isolated from burn patients. Burns. 2016 Aug;42(5):1116-1120.
11. Neamah AA. Molecular Detection of virulence factor genes in Pseudomonas aeruginosa isolated from human and animals in Diwaniya province. Kufa J Vet Med Sic. 2017;8:218-30.
12. Rumbaugh KP, Hamood AN, Griswold JA. Analysis ofPseudomonas aeruginosaClinical Isolates for Possible Variations within the Virulence Genes Exotoxin A and Exoenzyme S.
J Surg Res. 1999;82(1):95-105.
13. Lee VT, Smith RS, Tümmler B, Lory S. Activities of Pseudomonas aeruginosa effectors secreted by the Type III secretion system in vitro and during infection. Infect Immun. 2005 Mar;73(3):1695-705. doi: 10.1128/IAI.73.3.1695-1705.2005.
14. Kaszab E, Szoboszlay S, Dobolyi C, Háhn J, Pék N, Kriszt B. Antibiotic resistance profiles and virulence markers of Pseudomonas aeruginosa strains isolated from composts. Bioresour Technol. 2011 Jan;102(2):1543-8. doi: 10.1016/j.biortech.2010.08.027.
15. Swietnicki W, Czarny A, Antkowiak L, Zaczynska E, Kolodziejczak M, Sycz J, et al. Identification of a potent inhibitor of type II secretion system from Pseudomonas aeruginosa. Biochem Biophys Res Commun. 2019;513(3):688-93.
16. Amirmozafari N, Fallah Mehrabadi J, Isazadieh K, Habibi A. Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitals. Iran J Med Microbiol. 2015;8(4):36-43.
17. Mashouf RY, Esmaeili R, Alikhani MY, Ghanbari M. Evaluation of exotoxin A gene and frequency of polymerase chain reaction sensitivity in detection of pseudomonas aeruginosa isolated from burn patients. Tehran Univ Med J. 2014;72(3):167-73.
18. Chai J, Sheng Z, Yang H, Diao L, Li L. Successful treatment of invasive burn wound infection with sepsis in patients with major burns. Chin Med J. 2000;113(12):1142-6.
19. Zhang P, Zou B, Liou YC, Huang C. The pathogenesis and diagnosis of sepsis post burn injury. Burns Trauma. 2021 Feb 4;9:tkaa047.
20. El-Din AB, EL-Nagdy M, Badr R, EL-Sabagh A. Pseudomonas aeruginosa exotoxin A: its role in burn wound infection and wound healing. Egypt J Plast Reconstr Surg. 2008;32:59-65.
21. Gang RK, Bang RL, Sanyal SC, Mokaddas E, Lari AR. Pseudomonas aeruginosa septicaemia in burns. Burns. 1999 Nov;25(7):611-6.
22. Ciofu O, Tolker-Nielsen T. Tolerance and Resistance of Pseudomonas aeruginosa Biofilms to Antimicrobial Agents-How P. aeruginosa Can Escape Antibiotics. Front Microbiol. 2019 May 3;10:913. doi: 10.3389/fmicb.2019.00913.
23. Kolak J, van Saene HK, de la Cal MA, Silvestre L, Peric M. Control of bacterial pneumonia during mechanical ventilation. Croat Med J. 2005 Apr;46(2):183-96.
24. Rossolini GM, Mantengoli E. Treatment and control of severe infections caused by multiresistant Pseudomonas aeruginosa. Clin Microbiol Infect. 2005 Jul;11 Suppl 4:17-32.
25. Zabihi T, Shahsanaei Goneirani M. Antibiotic resistance patterns in Pseudomonas aeruginosa strains isolated from patients hospitalized in different hospital wards. Int J Med Rev. 2017;4(2):40-2.
26. Liu PV. Extracellular toxins of Pseudomonas aeruginosa. J Infect Dis. 1974 Nov;130 Suppl(0):S94-9.
27. Ghanbarzadeh Corehtash Z, Khorshidi A, Firoozeh F, Akbari H, Mahmoudi Aznaveh A. Biofilm Formation and Virulence Factors Among Pseudomonas aeruginosa Isolated From Burn Patients. Jundishapur J Microbiol. 2015 Oct 21;8(10):e22345. doi: 10.5812/jjm.22345.
28. Badr R, Nagdy M, Sabagh A, Din A. Pseudomonas aeruginosa exotoxin a as a virulence factor in burn wound infections. Egypt J Med Microbiol 2008;17(1):125-33.
29. Wolf P, Elsässer-Beile U. Pseudomonas exotoxin A: from virulence factor to anti-cancer agent. Int J Med Microbiol. 2009 Mar;299(3):161-76. doi: 10.1016/j.ijmm.2008.08.003.
30. Pastan I. Immunotoxins containing Pseudomonas exotoxin A: a short history. Cancer Immunol Immunother. 2003 May;52(5):338-41.
31. Pavlovskis OR, Iglewski BH, Pollack M. Mechanism of action of Pseudomonas aeruginosa exotoxin A in experimental mouse infections: adenosine diphosphate ribosylation of elongation factor 2. Infect Immun. 1978 Jan;19(1):29-33.
32. Michalska M, Wolf P. Pseudomonas Exotoxin A: optimized by evolution for effective killing. Front Microbiol. 2015 Sep 15;6:963.
33. Stuart RK, Pollack M. Pseudomonas aeruginosa exotoxin A inhibits proliferation of human bone marrow progenitor cells in vitro. Infect Immun. 1982 Oct;38(1):206-11.
34. Furuya N, Hirakata Y, Tomono K, Matsumoto T, Tateda K, Kaku M, Yamaguchi K. Mortality rates amongst mice with endogenous septicaemia caused by Pseudomonas aeruginosa isolates from various clinical sources. J Med Microbiol. 1993 Aug;39(2):141-6.
35. Wagener BM, Hu R, Wu S, Pittet JF, Ding Q, Che P. The Role of Pseudomonas aeruginosa Virulence Factors in Cytoskeletal Dysregulation and Lung Barrier Dysfunction. Toxins (Basel). 2021 Nov 2;13(11):776.
36. Kadhim SR. Genetic Study of Txo A Gene in Pseudomonas aeruginosa Isolated from Burn Wound Infections. Res J Pharm Tech. 2020;13(11):5301-5.
37. Bjorn MJ, Vasil ML, Sadoff JC, Iglewski BH. Incidence of exotoxin production by Pseudomonas species. Infect Immun. 1977 Apr;16(1):362-6. doi: 10.1128/iai.16.1.362-366.1977.
38. Azimi S, Kafil HS, Baghi HB, Shokrian S, Najaf K, Asgharzadeh M, Yousefi M, Shahrivar F, Aghazadeh M. Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran. GMS Hyg Infect Control. 2016 Feb 22;11:Doc04.
39. Amini B, Kamali M, Zarei Mahmod Abadi A, Mortazavi Y, Ebrahim Habibi A, Bayat E, et al. Cloning of catalytic domain of exotoxin a from Pseudomonas aeruginosa. J Adv Med Biomed Res. 2010;18(71):24-33.
40. Qin X, Emerson J, Stapp J, Stapp L, Abe P, Burns JL. Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis. J Clin Microbiol. 2003 Sep;41(9):4312-7.
41. Hummel A, Unger G. Nachweis von Pseudomonas aeruginosa aus Bronchial- und Trachealsekreten mittels PCR durch Amplifikation des Exotoxin A-Genes [Detection of Pseudomonas aeruginosa in bronchial and tracheal aspirates by PCR by amplification of the exotoxin A gene]. Zentralbl Hyg Umweltmed. 1998 Dec;201(4-5):349-55. German. PMID: 9916289.
42. Zarei O, Shokoohizadeh L, Hossainpour H, Alikhani MY. Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR. BMC Res Notes. 2018 Sep 15;11(1):668.

آمار
تعداد مشاهده مقاله: 8,402
تعداد دریافت فایل اصل مقاله: 645


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب