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Cytotoxic Effect of Nano Fast Cement and ProRoot Mineral Trioxide Aggregate on L-929 Fibroblast Cells: an in vitro Study | ||
| Journal of Dentistry | ||
| مقاله 3، دوره 23، شماره 1 - شماره پیاپی 74، خرداد 2022، صفحه 13-19 اصل مقاله (413 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/dentjods.2021.87208.1239 | ||
| نویسندگان | ||
| Mohammadreza Nabavizadeh1؛ Fariborz Moazami1؛ Ahmad Gholami2؛ Vahid Mehrabi1؛ Yasamin Ghahramani* 3 | ||
| 1Dept. of Endodontic, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| 2Biotechnology Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| 3Dept. of Endodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| چکیده | ||
| Statement of the Problem: Endodontic materials that are placed in direct contact with living tissues should be biocompatible. The cytotoxicity of Nano Fast Cement (NFC) compared to ProRoot Mineral Trioxide Aggregate (ProRoot MTA) must be evaluated. Purpose: ThisIn vitro study aimed to assess the cytotoxic effects of NFC in comparison to ProRoot MTA on L-929 mouse fibroblast cells. Materials and Method: In this animal study,L-929 mouse fibroblast cells were grown in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) in an atmosphere of 5% co₂/95% air at 37 C̊. A total of 10⁴ cells from the fourth collection were plated in each well of a 96-well micro-titer plate. Materials were mixed according to the manufacturer’s instruction and placed into the related plastic molds with 5 mm diameter and 3 mm height. After 24 hours and a complete setting, the extracts of the tested materials were produced at six different concentrations and placed in the related wells. Cells in DMEM served as the negative control group. DMEM alone was used as the positive control group. Methyl-thiazoltetrazolium (MTT) colorimetric assay was conducted after 24, 48, and 72 hours. The absorbance values were measured by ELISA plate reader at 540 nm wavelength. Three-way analysis of variance, post-hoc Tukey, LSD, and independent t-test were used for the statistical analyses using SPSS software, version 16.0. Results: There was no statically significant difference between MTA and NFC in cell viability values at different concentrations and different time intervals (p = 0.649). Viability values were significantly decreased after 72 hours, but there was no significant difference between the first and second MTT assays (p = 0.987). Cytotoxicity significantly increased at concentrations higher than 6.25 µɡ/ml. Conclusion: Cytotoxicity depends on time, concentration, and cement composition. There was no statistically significant difference between NFC and MTA concerning their cytotoxic effects on L-929 mouse fibroblast cells. | ||
| کلیدواژه‌ها | ||
| Mineral Trioxide Aggregate؛ Cytotoxicity؛ Endodontics؛ Cement | ||
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| مراجع | ||
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[1] Abdullah D, Ford TP, Papaioannou S, Nicholson J, McDonald F. An evaluation of accelerated Portland cement as a restorative material. Biomaterials. 2002; 23: 4001-4010.
[2] Perez AL, Spears R, Gutmann JL, Opperman LA. Osteoblasts and MG‐63 osteosarcoma cells behave differently when in contact with ProRoot™ MTA and White MTA. Int Endod J. 2003; 36: 564-570.
[3] Koh ET, McDonald F, Ford TR, Torabinejad M. Cellular response to mineral trioxide aggregate. JOE. 1998; 24: 543-547.
[4] Mitchell PJ, Ford TP, Torabinejad M, McDonald F. Osteoblast biocompatibility of mineral trioxide aggregate. Biomaterials. 1999; 20: 167-173.
[5] Zhu Q, Haglund R, Safavi KE, Spangberg LS. Adhesion of human osteoblasts on root-end filling materials. JOE. 2000; 26: 404-406.
[6] Keiser K, Johnson CC, Tipton DA. Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts. JOE. 2000; 26: 288-291.
[7] De Deus G, Ximenes R, Gurgel‐Filho ED, Plotkowski MC, Coutinho‐Filho T. Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod J. 2005; 38: 604-609.
[8] Ribeiro DA, Sugui MM, Matsumoto MA, Duarte MA, Marques ME, Salvadori DM. Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro. Oral Surg Oral Med Oral Pathol Oral Radio Endod. 2006; 101: 258-261.
[9] AlAnezi AZ, Jiang J, Safavi KE, Spangberg LS, Zhu Q. Cytotoxicity evaluation of endosequence root repair material. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010; 109: e122-e125.
[10] Lee SJ, Monsef M, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. JOE. 1993; 19: 541-544.
[11] Aqrabawi J. Endodontics: Sealing ability of amalgam, super EBA cement, and MTA when used as retrograde filling materials. Brit Dent J. 2000; 188: 266.
[12] Torabinejad M, Watson TF, Ford TP. Sealing ability of a mineral trioxide aggregate when used as a root end filling material. JOE. 1993; 19: 591-595.
[13] Torabinejad M, Hong CU, Ford TP, Kettering JD. Antibacterial effects of some root end filling materials. JOE. 1995; 21: 403-406.
[14] Estrela C, Bammann LL, Estrela CR, Silva RS, Pécora JD. Antimicrobial and chemical study of MTA, Portland cement, calcium hydroxide paste, Sealapex and Dycal. Braz Dent J. 2000; 11: 3-9.
[15] Krastl G, Allgayer N, Lenherr P, Filippi A, Taneja P, Weiger R. Tooth discoloration induced by endodontic materials: a literature review. Dent Traumatol. 2013; 29: 2-7.
[16] Johnson BR. Considerations in the selection of a root-end filling material. Oral Surg Oral Med Oral Patho Oral Radio Endod. 1999; 87: 398-404.
[17] Piedad N, Zuleta F, Velasquez P, Vicente-Salar N, Reig JA. άH-Dicalcium silicate bone cement doped with tricalcium phosphate: characterization, bioactivity and biocompatibility. J Material Sci Mater Med. 2014; 25: 445-452.
[18] Laurent P, Camps J, About I. Biodentine induces TGF‐β1 release from human pulp cells and early dental pulp mineralization. Int Endod J. 2012; 45: 439-448.
[19] Sanaee M , Danesh Manesh H, Janghorban K, Sanaee R, Kooshesh L, Ghahramani Y, et al. The influence of particle size and multi-walled carbon nanotube on physical properties of mineral trioxide aggregate. IOP Science. 2019; 6: 1-10.
[20] Zhou HM, Shen Y, Wang ZJ, Li L, Zheng YF, Häkkinen L, et al. In vitro cytotoxicity evaluation of a novel root repair material. JOE. 2013; 39: 478-483.
[21] Abbaszadegan A, Gholami A, Ghahramani Y, Ghareghan R, Ghareghan M, Kazemi A, et al. Antimicrobial and cytotoxic activity of Cuminum cyminum as an intracanal medicament compared to chlorhexidine gel. Iran Endod J. 2016; 11: 44.
[22] Jaberiansari Z, Naderi S, Tabatabaei FS. Cytotoxic effects of various mineral trioxide aggregate formulations, calcium-enriched mixture and a new cement on human pulp stem cells. Iran Endod J. 2014; 9: 271.
[23] Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunomethod. 1983; 65: 55-63.
[24] Ghoddusi J, Afshari JT, Donyavi Z, Brook A, Disfani R, Esmaeelzadeh M. Cytotoxic effect of a new endodontic cement and mineral trioxide aggregate on L929 line culture. Iran Endod J. 2008; 3: 17.
[25] Gorduysus M, Avcu N, Gorduysus O, Pekel A, Baran Y, Avcu F, et al. Cytotoxic effects of four different endodontic materials in human periodontal ligament fibroblasts. JOE. 2007; 33: 1450-1454.
[26] Saberi EA, Karkehabadi H, Mollashahi NF. Cytotoxicity of various endodontic materials on stem cells of human apical papilla. Iran Endod J. 2016; 11: 17.
[27] Camilleri J, Sorrentino F, Damidot D. Characterization of un-hydrated and hydrated bioaggregate and mta angelus. Clin Oral Investig. 2015; 19: 689-698.
[28] Yoshino P, Nishiyama CK, Modena KC, Santos CF, Sipert CR. In vitro cytotoxicity of white MTA, MTA Fillapex® and Portland cement on human periodontal ligament fibroblasts. Braz Dent J. 2013; 24: 111-116.
[29] Nelson DL, Cox MM. Lehninger Principle of Biochemistry. 5th ed. W.H.Freeman: Gordonsville; 2008. p.1071.
[30] Lee BN, Lee KN, Koh JT, Min KS, Chang HS, Hwang IN, et al. Effects of 3 endodontic bioactive cements on osteogenic differentiation in mesenchymal stem cells. JOE. 2014; 40: 1217-1222.
[31] Aguirre A, González A, Planell JA, Engel E. Extracellular calcium modulates in vitro bone marrow-derived Flk-1+ CD34+ progenitor cell chemotaxis and differentiation through a calcium-sensing receptor. Biochem Biophys Res Commun. 2010; 393: 156-161.
[32] Wondergem R, Ecay TW, Mahieu F, Owsianik G, Nilius B. HGF/SF and menthol increase human glioblastoma cell calcium and migration. Biochem Biophys Res Commun. 2008; 372: 210-215. | ||
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