LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,149
تعداد مقالات10,518
تعداد مشاهده مقاله45,415,424
تعداد دریافت فایل اصل مقاله11,291,212
Sharafi-Badr, Paria, Karoobi, Sepideh, Monsef-Esfahani, Hamid Reza, Ghahremani, Mohammad Hossein, Adhami, Hamid-Reza. (1400). In vitro Cytotoxic Screening of Different Parts from Ornithogalum bungei on Selected Cancer Cells. سامانه مدیریت نشریات علمی, 47(1), 63-72. doi: 10.30476/ijms.2021.89521.2037
Paria Sharafi-Badr; Sepideh Karoobi; Hamid Reza Monsef-Esfahani; Mohammad Hossein Ghahremani; Hamid-Reza Adhami. "In vitro Cytotoxic Screening of Different Parts from Ornithogalum bungei on Selected Cancer Cells". سامانه مدیریت نشریات علمی, 47, 1, 1400, 63-72. doi: 10.30476/ijms.2021.89521.2037
Sharafi-Badr, Paria, Karoobi, Sepideh, Monsef-Esfahani, Hamid Reza, Ghahremani, Mohammad Hossein, Adhami, Hamid-Reza. (1400). 'In vitro Cytotoxic Screening of Different Parts from Ornithogalum bungei on Selected Cancer Cells', سامانه مدیریت نشریات علمی, 47(1), pp. 63-72. doi: 10.30476/ijms.2021.89521.2037
Sharafi-Badr, Paria, Karoobi, Sepideh, Monsef-Esfahani, Hamid Reza, Ghahremani, Mohammad Hossein, Adhami, Hamid-Reza. In vitro Cytotoxic Screening of Different Parts from Ornithogalum bungei on Selected Cancer Cells. سامانه مدیریت نشریات علمی, 1400; 47(1): 63-72. doi: 10.30476/ijms.2021.89521.2037

In vitro Cytotoxic Screening of Different Parts from Ornithogalum bungei on Selected Cancer Cells

Iranian Journal of Medical Sciences
مقاله 9، دوره 47، شماره 1، فروردین 2022، صفحه 63-72    اصل مقاله (2.43 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2021.89521.2037
نویسندگان
Paria Sharafi-Badr1؛ Sepideh Karoobi2؛ Hamid Reza Monsef-Esfahani1؛ Mohammad Hossein Ghahremani2، 3؛ Hamid-Reza Adhami* 1
1Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3Toxicology and Poisoning Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Background: Natural products comprise a large section of pharmaceutical agents in the field of cancer therapy. In the present study, the organic extracts and fractions of various parts of Ornithogalum bungei were investigated for in vitro cytotoxic properties on three human cancer cell lines, hepatocellular carcinoma (HepG2), prostate cancer (PC3), and leukemia (K562) cells.
Methods: The present experimental study was conducted at Tehran University of Medical Sciences (Tehran, Iran) during 2017-2019. Separately extracted plant materials, including bulbs, stems, and flowers of O. bungei were assessed by the tetrazolium dye-based colorimetric assay (MTT). The selected extracts were submitted to fractionation using vacuum liquid chromatography and after MTT assay, the half maximal inhibitory concentration (IC50 (value for each fraction was determined. The data were analyzed using One-way ANOVA followed by Tukey’s post hoc test. p Results: The cytotoxicity of the bulb’s methanol extract and the dichloromethane extract of aerial parts increased in a concentration-dependent manner. Additionally, cell viability decreased in a dose-dependent manner. In the HepG2 cell line, the best IC50 values of fractions from DCM extracts of aerial parts were determined to be 19.8±10.2 µg/mL after 24 hours of exposure and 19.39±6.4 µg/mL following 48 hours of exposure. In the PC3 cell line, after 48 hours of exposure, the IC50 values of fractions were unaccountable, while the percentage of inhibition for A6 to A11 in 24 hours of exposure was more than 40 µg/mL.
Conclusion: O. bungei growing in Iran showed significant potentials as a cytotoxic agent with selective effects on different cancer cell lines.
کلیدواژه‌ها
Ornithogalum bungei Boiss؛ Hyacinthaceae؛ Biological products؛ HepG2, PC3, K562 cells
سایر فایل های مرتبط با مقاله
مراجع
  1. Siegel R, Ward E, Brawley O, Jemal A. Cancer statistics, 2011: the impact of eliminating socioeconomic and racial disparities on premature cancer deaths. CA Cancer J Clin. 2011;61:212-36. doi: 10.3322/caac.20121. PubMed PMID: 21685461.
  2. Bucar F, Wube A, Schmid M. Natural product isolation--how to get from biological material to pure compounds. Nat Prod Rep. 2013;30:525-45. doi: 10.1039/c3np20106f. PubMed PMID: 23396532.
  3. Savithramma N, Rao ML, Suhrulatha D. Screening of medicinal plants for secondary metabolites. Middle-East Journal of Scientific Research. 2011;8:579-84.
  4. Bhanot A, Sharma R, Noolvi MN. Natural sources as potential anti-cancer agents: A review. International journal of phytomedicine. 2011;3:09.
  5. Chen R, Meng F, Liu Z, Chen R, Zhang M. Antitumor activities of different fractions of polysaccharide purified from Ornithogalum caudatum Ait. Carbohydrate Polymers. 2010;80:845-51.
  6. Tang Y, Li N, Duan JA, Tao W. Structure, bioactivity, and chemical synthesis of OSW-1 and other steroidal glycosides in the genus Ornithogalum. Chem Rev. 2013;113:5480-514. doi: 10.1021/cr300072s. PubMed PMID: 23530990.
  7. Zheng D, Guan Y, Chen X, Xu Y, Chen X, Lei P. Synthesis of cholestane saponins as mimics of OSW-1 and their cytotoxic activities. Bioorg Med Chem Lett. 2011;21:3257-60. doi: 10.1016/j.bmcl.2011.04.030. PubMed PMID: 21530251.
  8. Mimaki Y, Kuroda M, Kameyama A, Sashida Y, Hirano T, Oka K, et al. Cholestane glycosides with potent cytostatic activities on various tumor cells from Ornithogalum saundersiae bulbs. Bioorganic & Medicinal Chemistry Letters. 1997;7:633-6. doi: 10.1016/S0960-894X(97)00071-1.
  9. Iguchi T, Kuroda M, Naito R, Watanabe T, Matsuo Y, Yokosuka A, et al. Cholestane glycosides from Ornithogalum saundersiae bulbs and the induction of apoptosis in HL-60 cells by OSW-1 through a mitochondrial-independent signaling pathway. J Nat Med. 2019;73:131-45. doi: 10.1007/s11418-018-1252-4. PubMed PMID: 30327993.
  10. Cheng SL, Jiang XL, Shi Y, Tian WS. Concise synthesis of the core structures of saundersiosides. Org Lett. 2015;17:2346-9. doi: 10.1021/acs.orglett.5b00821. PubMed PMID: 25937005.
  11. Andriamisaina N, Mitaine-Offer AC, Miyamoto T, Tanaka C, Paululat T, Lirussi F, et al. Steroidal glycosides from Ornithogalum dubium Houtt. Phytochemistry. 2019;160:78-84. doi: 10.1016/j.phytochem.2019.01.013. PubMed PMID: 30743238.
  12. Iguchi T, Kuroda M, Naito R, Watanabe T, Matsuo Y, Yokosuka A, et al. Structural Characterization of Cholestane Rhamnosides from Ornithogalum saundersiae Bulbs and Their Cytotoxic Activity against Cultured Tumor Cells. Molecules. 2017;22. doi: 10.3390/molecules22081243. PubMed PMID: 28757596; PubMed Central PMCID: PMCPMC6152286.
  13. Ying W, Yan-Ling W, Li-Hua L, Ji-Xing N. Protective effect of Ornithogalum saundersiae Ait (Liliaceae) against acetaminophen-induced acute liver injury via CYP2E1 and HIF-1α. Chinese Journal of Natural Medicines. 2012;10:177-84. doi: 10.1016/j.jep.2010.08.025.
  14. Zhou Y, Garcia-Prieto C, Carney DA, Xu RH, Pelicano H, Kang Y, et al. OSW-1: a natural compound with potent anticancer activity and a novel mechanism of action. J Natl Cancer Inst. 2005;97:1781-5. doi: 10.1093/jnci/dji404. PubMed PMID: 16333034.
  15. Chen QW, Zhang X, Gong T, Gao W, Yuan S, Zhang PC, et al. Structure and bioactivity of cholestane glycosides from the bulbs of Ornithogalum saundersiae Baker. Phytochemistry. 2019;164:206-14. doi: 10.1016/j.phytochem.2019.05.016. PubMed PMID: 31177053.
  16. Tilaoui M, Ait Mouse H, Jaafari A, Zyad A. Comparative Phytochemical Analysis of Essential Oils from Different Biological Parts of Artemisia herba alba and Their Cytotoxic Effect on Cancer Cells. PLoS One. 2015;10:e0131799. doi: 10.1371/journal.pone.0131799. PubMed PMID: 26196123; PubMed Central PMCID: PMCPMC4510584.
  17. Delazar A, Nazifi E, Movafeghi A, Nahar L, Nazemiyeh H, Moghadam S, et al. GC-MS analysis of Ornithogalum procerum. DARU Journal of Pharmaceutical Sciences. 2015;17:33-6.
  18. Xiu-Ping C, Jian-Hua Z, De-You Q, Jun-Gui D. Steroidal Saponins and Flavonoids from Ornithogalum thyrsoides. Natural Product Research & Development. 2012;24. doi:10.1021/np040108i.
  19. Koyuncu I, Gonel A, Akdag A, Yilmaz MA. Identification of phenolic compounds, antioxidant activity and anti-cancer effects of the extract obtained from the shoots of Ornithogalum narbonense L. Cell Mol Biol (Noisy-le-grand). 2018;64:75-83. doi: 10.14715/cmb/2018.64.1.14. PubMed PMID: 29412798.
  20. Jafari S, Akhani H. Plants of jahan nama protected area, Golestan province, N. Iran. Pakistan journal of botany. 2008;40:1533-54.
  21. Siadati S, Moradi H, Attar F, Etemad V, Hamzeh’ee B, Naqinezhad A. Botanical diversity of Hyrcanian forests; a case study of a transect in the Kheyrud protected lowland mountain forests in northern Iran. Phytotaxa. 2010;7:1-18. doi: 10.11646/phytotaxa.7.1.1.
  22. Ertugrul K, Uysal T, Dural H, Tugay O, Demirelma H. Türkiye Ornithogalum L.(Hyacinthaceae) Cinsinin Revizyonu. TÜBİTAK KBAG Proje. 2014. Turkiy.
  23. van Meerloo J, Kaspers GJ, Cloos J. Cell sensitivity assays: the MTT assay. Methods Mol Biol. 2011;731:237-45. doi: 10.1007/978-1-61779-080-5_20. PubMed PMID: 21516412.
  24. Dundek P, Holík L, Rohlík T, Hromádko L, Vranová V, Rejšek K, et al. Methods of plant root exudates analysis: a review. Acta univ agric et silvic Mendel Brun. 2011;59:241-6. doi: 10.11118/actaun201159030241.
  25. Chen R, Meng F, Liu Z, Chen R, Zhang M. Antitumor activities of different fractions of polysaccharide purified from Ornithogalum caudatum Ait. Carbohydrate Polymers. 2010;80:845-51. doi:10.1016/j.carbpol.2009.12.042.
  26. Yang Xt, LI Jt, WU Lj. Polysaccharide Assay Study of Ornithogalum caudatum Ait. Modern Chinese Medicine. 2011:07.
  27. Fizazi K, De Bono JS, Flechon A, Heidenreich A, Voog E, Davis NB, et al. Randomised phase II study of siltuximab (CNTO 328), an anti-IL-6 monoclonal antibody, in combination with mitoxantrone/prednisone versus mitoxantrone/prednisone alone in metastatic castration-resistant prostate cancer. Eur J Cancer. 2012;48:85-93. doi: 10.1016/j.ejca.2011.10.014. PubMed PMID: 22129890.
  28. He G, Karin M. NF-kappaB and STAT3 - key players in liver inflammation and cancer. Cell Res. 2011;21:159-68. doi: 10.1038/cr.2010.183. PubMed PMID: 21187858; PubMed Central PMCID: PMCPMC3193410.
  29. Ramesh V, Selvarasu K, Pandian J, Myilsamy S, Shanmugasundaram C, Ganesan K. NFkappaB activation demarcates a subset of hepatocellular carcinoma patients for targeted therapy. Cell Oncol (Dordr). 2016;39:523-36. doi: 10.1007/s13402-016-0294-4. PubMed PMID: 27562587.
  30. Ogata S, Kameda K, Kono T, Ozeki Y, Hashimoto H, Tominaga S, et al. Expressions of ATF6, XBP1, and GRP78 in normal tissue, atypical adenomatous hyperplasia, and adenocarcinoma of the lung. Hum Pathol. 2019;83:22-8. doi: 10.1016/j.humpath.2018.08.009. PubMed PMID: 30121368.
  31. Garcia-Prieto C, Riaz Ahmed KB, Chen Z, Zhou Y, Hammoudi N, Kang Y, et al. Effective killing of leukemia cells by the natural product OSW-1 through disruption of cellular calcium homeostasis. J Biol Chem. 2013;288:3240-50. doi: 10.1074/jbc.M112.384776. PubMed PMID: 23250754; PubMed Central PMCID: PMCPMC3561545.
  32. Inami Y, Waguri S, Sakamoto A, Kouno T, Nakada K, Hino O, et al. Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells. J Cell Biol. 2011;193:275-84. doi: 10.1083/jcb.201102031. PubMed PMID: 21482715; PubMed Central PMCID: PMCPMC3080263.
  33. Luk JM, Lam CT, Siu AF, Lam BY, Ng IO, Hu MY, et al. Proteomic profiling of hepatocellular carcinoma in Chinese cohort reveals heat-shock proteins (Hsp27, Hsp70, GRP78) up-regulation and their associated prognostic values. Proteomics. 2006;6:1049-57. doi: 10.1002/pmic.200500306. PubMed PMID: 16400691.
  34. Derks S, Diosdado B. Personalized cancer medicine: next steps in the genomic era. Cell Oncol (Dordr). 2015;38:1-2. doi: 10.1007/s13402-015-0221-0. PubMed PMID: 25720594; PubMed Central PMCID: PMCPMC4359352.
آمار
تعداد مشاهده مقاله: 5,399
تعداد دریافت فایل اصل مقاله: 2,843


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب