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Mahmoodi Khatonabadi, Somayeh, Salami, Siamak, Mirfakhraie, Reza, Atabakhshian, Roya, Sirati-Sabet, Majid, Yaghmaei, Bahram Gholamali, Ghafghazi, Shiva, Ziai, Seyed Ali. (1401). Umbelliprenin Inhibited Angiogenesis and Metastasis of MDA-MB-231 Cell Line through Downregulation of CoCl2 / EGFMediated PI3K / AKT / ERK Signaling. سامانه مدیریت نشریات علمی, 13(2), 226-236. doi: 10.30476/mejc.2021.86492.1347
Somayeh Mahmoodi Khatonabadi; Siamak Salami; Reza Mirfakhraie; Roya Atabakhshian; Majid Sirati-Sabet; Bahram Gholamali Yaghmaei; Shiva Ghafghazi; Seyed Ali Ziai. "Umbelliprenin Inhibited Angiogenesis and Metastasis of MDA-MB-231 Cell Line through Downregulation of CoCl2 / EGFMediated PI3K / AKT / ERK Signaling". سامانه مدیریت نشریات علمی, 13, 2, 1401, 226-236. doi: 10.30476/mejc.2021.86492.1347
Mahmoodi Khatonabadi, Somayeh, Salami, Siamak, Mirfakhraie, Reza, Atabakhshian, Roya, Sirati-Sabet, Majid, Yaghmaei, Bahram Gholamali, Ghafghazi, Shiva, Ziai, Seyed Ali. (1401). 'Umbelliprenin Inhibited Angiogenesis and Metastasis of MDA-MB-231 Cell Line through Downregulation of CoCl2 / EGFMediated PI3K / AKT / ERK Signaling', سامانه مدیریت نشریات علمی, 13(2), pp. 226-236. doi: 10.30476/mejc.2021.86492.1347
Mahmoodi Khatonabadi, Somayeh, Salami, Siamak, Mirfakhraie, Reza, Atabakhshian, Roya, Sirati-Sabet, Majid, Yaghmaei, Bahram Gholamali, Ghafghazi, Shiva, Ziai, Seyed Ali. Umbelliprenin Inhibited Angiogenesis and Metastasis of MDA-MB-231 Cell Line through Downregulation of CoCl2 / EGFMediated PI3K / AKT / ERK Signaling. سامانه مدیریت نشریات علمی, 1401; 13(2): 226-236. doi: 10.30476/mejc.2021.86492.1347

Umbelliprenin Inhibited Angiogenesis and Metastasis of MDA-MB-231 Cell Line through Downregulation of CoCl2 / EGFMediated PI3K / AKT / ERK Signaling

Middle East Journal of Cancer
مقاله 3، دوره 13، شماره 2 - شماره پیاپی 50، تیر 2022، صفحه 226-236    اصل مقاله (2.67 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2021.86492.1347
نویسندگان
Somayeh Mahmoodi Khatonabadi1؛ Siamak Salami1؛ Reza Mirfakhraie2؛ Roya Atabakhshian1؛ Majid Sirati-Sabet1؛ Bahram Gholamali Yaghmaei1؛ Shiva Ghafghazi3؛ Seyed Ali Ziai* 3
1Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده
Background: Breast cancer is known to be one of the most prevalent malignancies in women worldwide. Umbelliprenin (UMB) is a naturally-occurring component derived from plant species, which has shown anticancer properties. The present study aimed to evaluate the effect of UMB on the PI3K / Akt / ERK signaling pathway and their products HIF-1α / VEGF in the MDA-MB-231 cell line.
Method: In this experimental study, the cytotoxic effect of UMB on MDA-MB- 231 cells was evaluated using the MTT assay and the UMB concentrations of IC5 and IC10 were selected for the signaling pathway study. MDA-MB-231 cells were stimulated with EGF and CoCl2 and UMB IC5 and IC10 effects on gene expression and translation was studied. PI3K / Akt / mTOR / S6K / Erk1 and 2 / 4E-BP1 / HIF-1α / HIF-1α/ EGFR / VEGFR and VEGF mRNA expression, and VEGF / HIF-1α proteins were evaluated employing real time polymerase chain reaction and western blot analysis, respectively.
Results: The concentrations of UMB in IC10 and IC5 were 20 and 10 μM, respectively. UMB, specifically IC10, significantly inhibited PI3K, ERK1, ERK2, Akt, mTOR, HIF1-α, HIF1-β mRNA, as well as HIF-1α and VEGF protein expression.
Conclusion: Our results suggested that UMB, a cytotoxic agent, inhibits PI3K / Akt / ERK signal pathway in the CoCl2 or EGF-stimulated MDA-MB-231 cells.
کلیدواژه‌ها
Umbelliprenin؛ MDA-MB-231؛ Cobalt Chloride؛ EGF؛ HIF1α؛ VEGF؛ Angiogenesis؛ Cancer
اصل مقاله

How to cite this article:

Mahmoodi Khatonabadi S, Salami S, Mirfakhraie R, Atabakhshian R, Sirati-Sabet M, Gholamali Yaghmaei B, et al. Umbelliprenin inhibited angiogenesis and metastasis of MDA-MB-231 cell line through downregulation of CoCl2 / EGF-mediated PI3K / AKT / ERK signaling. Middle East J Cancer. 2022;13(2):226-36-p. doi: 10.30476/mejc.2021. 86492.1347.

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