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Gebril, Sara, Elkhawaga, Om-Ali. (1400). Safe Combination of Cisplatin and Metformin Reverts the Malignant Ascites in a Mouse Model to a Solid Tumor by Downregulation of ΔNp63 and Induces Tumor Dormancy via mTOR/ p21 Mechanism. سامانه مدیریت نشریات علمی, 13(1), 43-55. doi: 10.30476/mejc.2021.86035.1321
Sara Gebril; Om-Ali Elkhawaga. "Safe Combination of Cisplatin and Metformin Reverts the Malignant Ascites in a Mouse Model to a Solid Tumor by Downregulation of ΔNp63 and Induces Tumor Dormancy via mTOR/ p21 Mechanism". سامانه مدیریت نشریات علمی, 13, 1, 1400, 43-55. doi: 10.30476/mejc.2021.86035.1321
Gebril, Sara, Elkhawaga, Om-Ali. (1400). 'Safe Combination of Cisplatin and Metformin Reverts the Malignant Ascites in a Mouse Model to a Solid Tumor by Downregulation of ΔNp63 and Induces Tumor Dormancy via mTOR/ p21 Mechanism', سامانه مدیریت نشریات علمی, 13(1), pp. 43-55. doi: 10.30476/mejc.2021.86035.1321
Gebril, Sara, Elkhawaga, Om-Ali. Safe Combination of Cisplatin and Metformin Reverts the Malignant Ascites in a Mouse Model to a Solid Tumor by Downregulation of ΔNp63 and Induces Tumor Dormancy via mTOR/ p21 Mechanism. سامانه مدیریت نشریات علمی, 1400; 13(1): 43-55. doi: 10.30476/mejc.2021.86035.1321

Safe Combination of Cisplatin and Metformin Reverts the Malignant Ascites in a Mouse Model to a Solid Tumor by Downregulation of ΔNp63 and Induces Tumor Dormancy via mTOR/ p21 Mechanism

Middle East Journal of Cancer
مقاله 5، دوره 13، شماره 1 - شماره پیاپی 49، فروردین 2022، صفحه 43-55    اصل مقاله (7.52 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2021.86035.1321
نویسندگان
Sara Gebril* ؛ Om-Ali Elkhawaga
Division of Biochemistry, Department of Chemistry, Faculty of Science, Mansoura University, Mansoura, Egypt
چکیده
Background: Currently, combination therapy has become the cornerstone of cancer treatment. The combination of different anticancer mechanisms can induce tumor cell quiescence. However, toxicity to normal tissue is the major limitation of existing combined drugs.
Method: In this experimental study, Ehrlich ascites carcinoma (EAC) inoculated into mice was targeted with just one dose of cisplatin and later doses of metformin, a safe antidiabetic drug with an anticancer effect, to maintain EAC cells in the quiescent state and secure a longer survival time without tumor recurrence.
Results: The group that underwent dual therapy developed a delayed solid tumor instead of a malignant ascites. The induction of chemo-quiescence in the EAC cells was proven by the downregulation of mechanistic target of rapamycin and the upregulation of cyclin-dependent kinase inhibitor 1 (p21) expressions. Intriguingly, the conversion of free neoplastic cells into a solid tumor was associated with a significant decrease in ΔNp63 immunostaining in EAC cells.
Conclusion: Taken together, a single dose of cisplatin followed by metformin doses could overcome the aggressiveness of malignant ascites by the conversion into a solid tumor, induction of chemo-quiescence, and the extension of survival time.
کلیدواژه‌ها
Chemo-quiescence؛ Ehrlich ascites carcinoma؛ Mechanistic target of rapamycin (mTOR)؛ Metformin؛ Cisplatin؛ ΔNp63
اصل مقاله

How to cite this article:

Gebril S, Elkhawaga OA. Safe combination of cisplatin and metformin reverts the malignant ascites in a mouse model to a solid tumor by downregulation of ΔNp63 and induces tumor dormancy via mTOR/ p21 mechanism. Middle East J Cancer. 2022;13(1):43-55. doi: 10.30476/mejc.2021.86035.1321.

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