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The relationship between serum levels of interleukin-2 and IL-8 with circulating microRNA-10b in patients with COVID-19 | ||
| Iranian Journal of Immunology | ||
| دوره 18، شماره 1، خرداد 2021، صفحه 65-73 اصل مقاله (618.25 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2021.88780.1904 | ||
| نویسندگان | ||
| Zahra Bagheri-Hosseinabadi1، 2؛ Hamid Ostad Ebrahim3؛ Fatemeh Bahrehmand4؛ Gholamhosein Taghipour5؛ Mitra Abbasifard* 6، 7 | ||
| 1Department of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran | ||
| 2Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran | ||
| 3Pediatric Department, Medicine Faculty, Non-Communicable Disease Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran | ||
| 4Department of Internal Medicine, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. | ||
| 5Department of Surgery, Ali-Ibn Abi-Talib hospital, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran | ||
| 6Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran | ||
| 7Department of Internal Medicine, Ali-Ibn Abi-Talib Hospital, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran | ||
| چکیده | ||
| Background: The role of cytokine storm in the immunopathogenesis of coronavirus disease 2019 (COVID-19) has been implicated. Objective: To determine the association of microRNA (miRNA)-10b and serum levels of IL-2 and IL-8 in patients with COVID-19. Methods: Blood samples were obtained from 33 COVID-19 patients and 29 healthy subjects. After RNA extraction and cDNA synthesis, the transcript level of miR-10b was determined by Real-time PCR. In addition, the serum levels of IL-2 and IL-8 were measured in subjects using ELISA. Results: The patient group comprised of 33 patients with COVID-19 (62.4 ± 3.7 years old), 13 (39%) males and 20 (61%) females. In the control group, 29 subjects (56.6 ± 1.6 years old), 9 (31%) males and 20 (69%) females, were included. The expression of miR-10b was significantly downregulated in the peripheral blood of COVID-19 patients in comparison to the healthy controls (fold change= 0.12, p < 0.0001). The levels of IL-2 (p < 0.001) and IL-8 (p < 0.001) were significantly increased in the serum samples of COVID-19 patients compared to the healthy subjects. The expression level of miR-10b was correlated significantly with the serum levels of IL-2 and IL-8 as well as with the age of patients, ESR and CRP levels. Conclusions: miR-10b is downregulated in the COVID-19 patients and might result in increased levels of IL-2 and IL-8, hence contributing to cytokine storm. | ||
| کلیدواژه‌ها | ||
| Coronavirus Disease 2019؛ MicroRNA؛ Cytokine Storm؛ IL-2؛ IL-18 | ||
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