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Fateen, Mohamed, Seif, Alia, Salama, Rasha, Shams, Ahmed, Amin, Dalia. (1400). The Relationship between the Connexin 32 and Connexin 43 Genes and the Pretreatment Stage and Short-term Follow-up of Patients with Acute Myeloid Leukemia. سامانه مدیریت نشریات علمی, 46(5), 347-354. doi: 10.30476/ijms.2020.84511.1477
Mohamed Fateen; Alia Seif; Rasha Salama; Ahmed Shams; Dalia Amin. "The Relationship between the Connexin 32 and Connexin 43 Genes and the Pretreatment Stage and Short-term Follow-up of Patients with Acute Myeloid Leukemia". سامانه مدیریت نشریات علمی, 46, 5, 1400, 347-354. doi: 10.30476/ijms.2020.84511.1477
Fateen, Mohamed, Seif, Alia, Salama, Rasha, Shams, Ahmed, Amin, Dalia. (1400). 'The Relationship between the Connexin 32 and Connexin 43 Genes and the Pretreatment Stage and Short-term Follow-up of Patients with Acute Myeloid Leukemia', سامانه مدیریت نشریات علمی, 46(5), pp. 347-354. doi: 10.30476/ijms.2020.84511.1477
Fateen, Mohamed, Seif, Alia, Salama, Rasha, Shams, Ahmed, Amin, Dalia. The Relationship between the Connexin 32 and Connexin 43 Genes and the Pretreatment Stage and Short-term Follow-up of Patients with Acute Myeloid Leukemia. سامانه مدیریت نشریات علمی, 1400; 46(5): 347-354. doi: 10.30476/ijms.2020.84511.1477

The Relationship between the Connexin 32 and Connexin 43 Genes and the Pretreatment Stage and Short-term Follow-up of Patients with Acute Myeloid Leukemia

Iranian Journal of Medical Sciences
مقاله 4، دوره 46، شماره 5، آذر 2021، صفحه 347-354    اصل مقاله (303.89 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2020.84511.1477
نویسندگان
Mohamed Fateen* 1؛ Alia Seif1؛ Rasha Salama2؛ Ahmed Shams1؛ Dalia Amin1
1Department of Clinical and Chemical Pathology, Cairo University, Cairo, Egypt
2Department of Clinical Oncology, Cairo University, Cairo, Egypt
چکیده
Background: Connexins (Cxs) are gap junction proteins involved in the communication between acute myeloid leukemia (AML) and stromal cells. They consist of intercellular channels termed “connexions”, which can cause uncontrolled cell proliferation if dysregulated. This study aimed to evaluate the expression levels of the Cx32 and Cx43 genes and their correlations with other prognostic markers in patients with AML.
Methods: This cross sectional study was performed on peripheral blood samples from 60 newly diagnosed patients with AML and 40 healthy control subjects at Kasr Alainy School of Medicine, Cairo University, from June 2016 to December 2017. The quantitative real-time polymerase chain reaction (qRT-PCR) test was used to examine the relative expression level of Cx43 and Cx32 genes in the patients and the control subjects. The Chi square test or the Fisher exact test was employed to examine the relationship between qualitative variables, while the independent t test or the Mann–Whitney test was employed for quantitative data. All the tests were two-tailed, and a P value of less than 0.05 was considered significant.
Results: Among the patients with AML, 65% had a high Cx32 expression level, whereas 63.3% had a low Cx43 expression level. There was a statistically significant difference in the fold change values of Cx32 and Cx43 expression between the patient group and the control group (P=0.009 vs P=0.013, respectively). There was a remarkable association between both Cxs and CD34 and HLA-DR cells.
Conclusion: Cx expression in samples may add to the diagnostic workup of AML. Although we found a negative correlation between Cx43 expression and the peripheral blood blast percentage, the response after the first induction of chemotherapy showed no significant relationship with Cx43 and Cx32.
کلیدواژه‌ها
Leukemia, Myeloid, Acute؛ Connexin 43؛ Gap junctions؛ Gene expression
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مراجع
  1. Yi S, Chen Y, Wen L, Yang L, Cui G. Expression of connexin 32 and connexin 43 in acute myeloid leukemia and their roles in proliferation. Oncol Lett. 2012;4:1003-7. doi: 10.3892/ol.2012.884. PubMed PMID: 23162640; PubMed Central PMCID: PMCPMC3499603.
  2. Kouzi F, Zibara K, Bourgeais J, Picou F, Gallay N, Brossaud J, et al. Disruption of gap junctions attenuates acute myeloid leukemia chemoresistance induced by bone marrow mesenchymal stromal cells. Oncogene. 2020;39:1198-212. doi: 10.1038/s41388-019-1069-y. PubMed PMID: 31649334; PubMed Central PMCID: PMCPMC7002301.
  3. Foss B, Tronstad KJ, Bruserud O. Connexin-based signaling in acute myelogenous leukemia (AML). Biochim Biophys Acta. 2010;1798:1-8. doi: 10.1016/j.bbamem.2009.10.014. PubMed PMID: 19883623.
  4. Singh AK, Cancelas JA. Gap Junctions in the Bone Marrow Lympho-Hematopoietic Stem Cell Niche, Leukemia Progression, and Chemoresistance. Int J Mol Sci. 2020;21. doi: 10.3390/ijms21030796. PubMed PMID: 31991829; PubMed Central PMCID: PMCPMC7038046.
  5. Begandt D, Good ME, Keller AS, DeLalio LJ, Rowley C, Isakson BE, et al. Pannexin channel and connexin hemichannel expression in vascular function and inflammation. BMC Cell Biol. 2017;18:2. doi: 10.1186/s12860-016-0119-3. PubMed PMID: 28124621; PubMed Central PMCID: PMCPMC5267334.
  6. Saito M, Asai Y, Imai K, Hiratoko S, Tanaka K. Connexin30.3 is expressed in mouse embryonic stem cells and is responsive to leukemia inhibitory factor. Sci Rep. 2017;7:42403. doi: 10.1038/srep42403. PubMed PMID: 28205646; PubMed Central PMCID: PMCPMC5304323.
  7. Maes M, Crespo Yanguas S, Willebrords J, Weemhoff JL, da Silva TC, Decrock E, et al. Connexin hemichannel inhibition reduces acetaminophen-induced liver injury in mice. Toxicol Lett. 2017;278:30-7. doi: 10.1016/j.toxlet.2017.07.007. PubMed PMID: 28687253; PubMed Central PMCID: PMCPMC5800489.
  8. Liu Y, Zhang X, Li ZJ, Chen XH. Up-regulation of Cx43 expression and GJIC function in acute leukemia bone marrow stromal cells post-chemotherapy. Leuk Res. 2010;34:631-40. doi: 10.1016/j.leukres.2009.10.013. PubMed PMID: 19910046.
  9. Zeijlemaker W, Grob T, Meijer R, Hanekamp D, Kelder A, Carbaat-Ham JC, et al. CD34(+)CD38(-) leukemic stem cell frequency to predict outcome in acute myeloid leukemia. Leukemia. 2019;33:1102-12. doi: 10.1038/s41375-018-0326-3. PubMed PMID: 30542144.
  10. Valdebenito S, Lou E, Baldoni J, Okafo G, Eugenin E. The Novel Roles of Connexin Channels and Tunneling Nanotubes in Cancer Pathogenesis. Int J Mol Sci. 2018;19. doi: 10.3390/ijms19051270. PubMed PMID: 29695070; PubMed Central PMCID: PMCPMC5983846.
  11. Reikvam H, Ryningen A, Saeterdal LR, Nepstad I, Foss B, Bruserud O. Connexin expression in human acute myeloid leukemia cells: identification of patient subsets based on protein and global gene expression profiles. Int J Mol Med. 2015;35:645-52. doi: 10.3892/ijmm.2014.2045. PubMed PMID: 25529637; PubMed Central PMCID: PMCPMC4314410.
  12. Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gralnick HR, et al. Proposals for the classification of the acute leukaemias. French-American-British (FAB) co-operative group. Br J Haematol. 1976;33:451-8. doi: 10.1111/j.1365-2141.1976.tb03563.x. PubMed PMID: 188440.
  13. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008;3:1101-8. doi: 10.1038/nprot.2008.73. PubMed PMID: 18546601.
  14. Yang S, Wen Q, Liu Y, Zhang C, Wang M, Chen G, et al. Increased expression of Cx43 on stromal cells promotes leukemia apoptosis. Oncotarget. 2015;6:44323-31. doi: 10.18632/oncotarget.6249. PubMed PMID: 26517241; PubMed Central PMCID: PMCPMC4792559.
  15. Zhang X, Liu Y, Si YJ, Chen XH, Li ZJ, Gao L, et al. Effect of Cx43 gene-modified leukemic bone marrow stromal cells on the regulation of Jurkat cell line in vitro. Leuk Res. 2012;36:198-204. doi: 10.1016/j.leukres.2011.10.001. PubMed PMID: 22030334.
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