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Abroudi, Mina, Dadashizadeh, Ghazaleh, Sam'e, Mohamadreza, Abbaszadeh Goudarzi, Kazem, Gholami, Omid, Shakiba, Abolfazl, Mahdian, Davood. (1401). Antiproliferative Effects of Different Concentrations of Auraptene on MCF7 Cancer Cell Line. سامانه مدیریت نشریات علمی, 13(3), 411-417. doi: 10.30476/mejc.2021.87927.1448
Mina Abroudi; Ghazaleh Dadashizadeh; Mohamadreza Sam'e; Kazem Abbaszadeh Goudarzi; Omid Gholami; Abolfazl Shakiba; Davood Mahdian. "Antiproliferative Effects of Different Concentrations of Auraptene on MCF7 Cancer Cell Line". سامانه مدیریت نشریات علمی, 13, 3, 1401, 411-417. doi: 10.30476/mejc.2021.87927.1448
Abroudi, Mina, Dadashizadeh, Ghazaleh, Sam'e, Mohamadreza, Abbaszadeh Goudarzi, Kazem, Gholami, Omid, Shakiba, Abolfazl, Mahdian, Davood. (1401). 'Antiproliferative Effects of Different Concentrations of Auraptene on MCF7 Cancer Cell Line', سامانه مدیریت نشریات علمی, 13(3), pp. 411-417. doi: 10.30476/mejc.2021.87927.1448
Abroudi, Mina, Dadashizadeh, Ghazaleh, Sam'e, Mohamadreza, Abbaszadeh Goudarzi, Kazem, Gholami, Omid, Shakiba, Abolfazl, Mahdian, Davood. Antiproliferative Effects of Different Concentrations of Auraptene on MCF7 Cancer Cell Line. سامانه مدیریت نشریات علمی, 1401; 13(3): 411-417. doi: 10.30476/mejc.2021.87927.1448

Antiproliferative Effects of Different Concentrations of Auraptene on MCF7 Cancer Cell Line

Middle East Journal of Cancer
مقاله 4، دوره 13، شماره 3 - شماره پیاپی 51، مهر 2022، صفحه 411-417    اصل مقاله (1.72 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2021.87927.1448
نویسندگان
Mina Abroudi1؛ Ghazaleh Dadashizadeh2؛ Mohamadreza Sam'e3؛ Kazem Abbaszadeh Goudarzi1؛ Omid Gholami1، 4؛ Abolfazl Shakiba1؛ Davood Mahdian* 1، 5
1Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
2Faculty of Health Sciences, McMaster University, Hamilton, Canada
3Student of Medicine, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
4Department of Pharmacology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
5Department of Pharmacology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
چکیده
Background: Auraptene is a coumarin derivative extracted from citrus species, such as lemon, grapefruit, and orange. To date, auraptene has shown antioxidant, antibacterial, anti-inflammatory, antiproliferative, antiapoptotic, and antitumor activities. Among these, antitumor activity has become more important over the recent years, while its underlying mechanism is not fully understood. The current study was conducted to evaluate the antiproliferative effect of auraptene and its mechanisms on MCF7 cell line.
Method: This experimental study investigated whether hesperidin affected the proliferation of MCF-7 human breast cancer cells. MCF7 cells were cultured in DMEM medium with 10% fetal bovine serum, 100 μg/ml streptomycin, and 100 units/ml penicillin. The cells were incubated in order to be treated with different concentrations of auraptene and time points. Subsequently, the amount of cytotoxicity and apoptosis was measured utilizing MTT and PI staining.
Results: The MTT assay revealed that auraptene had a significant effect on cell viability and induced apoptosis in MCF7 cells at concentrations of 75, 100, 130, 170, and 200 μM.
Conclusion: In this study, through the induction of apoptosis, auraptene prevented the growth and inhibited the proliferation of MCF7 cells at high concentrations in a dose-dependent manner. However, further investigation is needed to reveal the mechanisms of auraptene concerning apoptosis induction.
کلیدواژه‌ها
Auraptene؛ Apoptosis؛ Cytotoxicity؛ Cancer؛ MCF7 cells؛ MTT
اصل مقاله

How to cite this article:

Abroudi M, Dadashizadeh G, Sam'e M, Abbaszadeh Goudarzi K, Gholami O, Shakiba A, et al. Antiproliferative effects of different concentrations of Auraptene on MCF7 cancer cell line. Middle East J Cancer. 2022;13(3):411-7. doi: 10.30476/mejc.2021.87927.1448.

مراجع
  1. Mahdian D, Hosseini A, Mousavi SH, Bihamta M, Vahedi MM. The Evaluation of the Effects of hydro-alcoholic extract of brassica oleracea (red cabbage) on growth inhibition and apoptosis induction in breast cancer cell line MCF-7. [In Persian] The Iranian Journal of Obstetrics, Gynecology and Infertility (JOGI). 2015; 18 (151): 1-11. doi: 10.22038/ijogi.2015.4624.
  2. Duo J, Ying GG, Wang GW, Zhang L. Quercetin inhibits human breast cancer cell proliferation and induces apoptosis via Bcl-2 and Bax regulation. Mol Med Rep. 2012; 5(6): 1453-6. doi: 10.3892/mmr.2012.845.
  3. Ghobrial IM, Witzig TE, Adjei AA. Targeting apoptosis pathways in cancer therapy. CA Cancer J Clin. 2005; 55(3): 178-94. doi: 10.3322/canjclin.55.3.178.
  4. Brown JM, Attardi LD. The role of apoptosis in cancer development and treatment response. Nat Rev Cancer. 2005; 5(3): 231-7. doi: 10.1038/nrc1560.
  5. Okada H, Mak TW. Pathways of apoptotic and non-apoptotic death in tumour cells. Nat Rev Cancer. 2004; 4(8): 592-603. doi: 10.1038/nrc1412.
  6. Baskar R, Lee KA, Yeo R, Yeoh KW. Cancer and radiation therapy: current advances and future directions. Int J Med Sci. 2012; 9(3): 193-9. doi: 10.7150/ijms.3635.
  7. Balunas MJ, Kinghorn AD. Drug discovery from medicinal plants. Life Sci. 2005; 78(5): 431-41. doi: 10.1016/j.lfs.2005.09.012.
  8. Furukawa Y, Okuyama S, Amakura Y, Watanabe S, Fukata T, Nakajima M, et al. Isolation and characterization of activators of ERK/MAPK from citrus plants. Int J Mol Sci. 2012; 13(2): 1832-45. doi: 10.3390/ijms13021832.
  9. Murakami A, Kuki W, Takahashi Y, Yonei H, Nakamura Y, Ohto Y, et al. Auraptene, a citrus coumarin, inhibits 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion in ICR mouse skin, possibly through suppression of superoxide generation in leukocytes. Jpn J Cancer Res. 1997; 88(5): 443-52. doi: 10.1111/j.1349-7006.1997.tb00402.x.
  10. Genovese S, Epifano F. Auraptene: a natural biologically active compound with multiple targets. Curr Drug Targets. 2011; 12(3): 381-6. doi: 10.2174/138945011794815248.
  11. Krishnan P, Kleiner-Hancock H. Effects of Auraptene on IGF-1 stimulated cell cycle progression in the human breast cancer cell line, MCF-7. Int J Breast Cancer. 2012; 2012:502092. doi: 10.1155/2012/502092.
  12. Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev. 1998; 56(11): 317-33. doi: 10.1111/j.1753-4887.1998.tb01670.x.
  13. Sahebkar A. Citrus auraptene: a potential multifunctional therapeutic agent for nonalcoholic fatty liver disease. Ann Hepatol. 2011; 10(4): 575-7.
  14. Mahdian D, Shafiee-Nick R, Mousavi SH. Different effects of adenylyl cyclase activators and phosphodiesterases inhibitors on cervical cancer (HeLa) and breast cancer (MCF-7) cells proliferation. Toxicol Mech Methods. 2014; 24(4): 307-14. doi: 10.3109/15376516.2014.898354.
  15. Mousavi SH, Davari AS, Iranshahi M, Sabouri-Rad S, Tayarani Najaran Z. Comparative analysis of the cytotoxic effect of 7-prenyloxycoumarin compounds and herniarin on MCF-7 cell line. Avicenna J Phytomed. 2015; 5(6): 520-30.
  16. Lee JC, Shin EA, Kim B, Kim BI, Chitsazian-Yazdi M, Iranshahi M, et al. Auraptene induces apoptosis via myeloid cell leukemia 1-mediated activation of caspases in PC3 and DU145 prostate cancer cells. Phytother Res. 2017; 31(6): 891-898. doi: 10.1002/ptr.5810.
  17. Gholami O. Umbelliprenin mediates its apoptotic effect by hormesis: A commentary. Dose Response. 2017; 15(2): 1559325817710035. doi: 10.1177/1559325817710035.
  18. Mori H, Niwa K, Zheng Q, Yamada Y, Sakata K, Yoshimi N. Cell proliferation in cancer prevention; effects of preventive agents on estrogen-related endometrial carcinogenesis model and on an in vitro model in human colorectal cells. Mutat Res. 2001; 480-481: 201-7. doi: 10.1016/s0027-5107(01)00200-7.
  19. Jun DY, Kim JS, Park HS, Han CR, Fang Z, Woo MH, et al. Apoptogenic activity of auraptene of Zanthoxylum schinifolium toward human acute leukemia Jurkat T cells is associated with ER stress-mediated caspase-8 activation that stimulates mitochondria-dependent or -independent caspase cascade. Carcinogenesis. 2007; 28(6): 1303-13. doi: 10.1093/carcin/bgm028.
  20. Zheng Q, Hirose Y, Yoshimi N, Murakami A, Koshimizu K, Ohigashi H, et al. Further investigation of the modifying effect of various chemopreventive agents on apoptosis and cell proliferation in human colon cancer cells. J Cancer Res Clin Oncol. 2002; 128(10): 539-46. doi: 10.1007/s00432-002-0373-y.
  21. Ansquer Y, Legrand A, Bringuier AF, Vadrot N, Lardeux B, Mandelbrot L, et al. Progesterone induces BRCA1 mRNA decrease, cell cycle alterations and apoptosis in the MCF7 breast cancer cell line. Anticancer Res. 2005; 25(1A): 243-8.
  22. Murakami A, Nakamura Y, Tanaka T, Kawabata K, Takahashi D, Koshimizu K, et al. Suppression by citrus auraptene of phorbol ester-and endotoxin-induced inflammatory responses: role of attenuation of leukocyte activation. Carcinogenesis. 2000; 21(10): 1843-50. doi: 10.1093/carcin/21.10.1843.
  23. Krishnan P, Yan KJ, Windler D, Tubbs J, Grand R, Li BD, et al. Citrus auraptene suppresses cyclin D1 and significantly delays N-methyl nitrosourea induced mammary carcinogenesis in female Sprague-Dawley rats. BMC Cancer. 2009; 9:259. doi: 10.1186/1471-2407-9-259.
  24. Sherr CJ, Roberts JM. CDK inhibitors: positive and negative regulators of G1-phase progression. Genes Dev. 1999; 13(12): 1501-12. doi: 10.1101/gad.13.12.1501.
  25. Murakami A, Wada K, Ueda N, Sasaki K, Haga M, Kuki W, et al. In vitro absorption and metabolism of a citrus chemopreventive agent, auraptene, and its modifying effects on xenobiotic enzyme activities in mouse livers. Nutr Cancer. 2000; 36(2): 191-9. doi: 10.1207/S15327914NC3602_8.
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