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Changizi, Vahid, Gharekhani, Vahideh, Motavaseli, Elaheh. (1400). Co-treatment with Ginsenoside 20(S)-Rg3 and Curcumin increases Radiosensitivity of MDA-MB-231 Cancer Cell Line. سامانه مدیریت نشریات علمی, 46(4), 291-297. doi: 10.30476/ijms.2020.83977.1334
Vahid Changizi; Vahideh Gharekhani; Elaheh Motavaseli. "Co-treatment with Ginsenoside 20(S)-Rg3 and Curcumin increases Radiosensitivity of MDA-MB-231 Cancer Cell Line". سامانه مدیریت نشریات علمی, 46, 4, 1400, 291-297. doi: 10.30476/ijms.2020.83977.1334
Changizi, Vahid, Gharekhani, Vahideh, Motavaseli, Elaheh. (1400). 'Co-treatment with Ginsenoside 20(S)-Rg3 and Curcumin increases Radiosensitivity of MDA-MB-231 Cancer Cell Line', سامانه مدیریت نشریات علمی, 46(4), pp. 291-297. doi: 10.30476/ijms.2020.83977.1334
Changizi, Vahid, Gharekhani, Vahideh, Motavaseli, Elaheh. Co-treatment with Ginsenoside 20(S)-Rg3 and Curcumin increases Radiosensitivity of MDA-MB-231 Cancer Cell Line. سامانه مدیریت نشریات علمی, 1400; 46(4): 291-297. doi: 10.30476/ijms.2020.83977.1334

Co-treatment with Ginsenoside 20(S)-Rg3 and Curcumin increases Radiosensitivity of MDA-MB-231 Cancer Cell Line

Iranian Journal of Medical Sciences
مقاله 7، دوره 46، شماره 4، مهر 2021، صفحه 291-297    اصل مقاله (1.8 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2020.83977.1334
نویسندگان
Vahid Changizi1؛ Vahideh Gharekhani* 2؛ Elaheh Motavaseli3
1Department of Radiotherapy, School of Medicine, Health Information Management Research Center, Tehran University of Medical Sciences, Tehran, Iran
2Department of Radiobiology, School of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran
3Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran, Iran
چکیده
Background: Breast cancer is the second most common cancer in women worldwide. Developing drugs increase the radiosensitivity effect of tumoral tissue, while protecting normal tissues has gained much attention. Ginsenoside Rg3, one of the active components of ginseng, has been shown to possess various pharmacological effects and antiproliferation activity on cancer cell lines. In this study, we assessed the anti-cancer effect of co-treatment with ginsenoside 20(S)-Rg3 and curcumin on MDA-MB-231 breast cancer cells with and without radiotherapy.
Methods: MTT assay was applied using different concentrations of ginsenoside 20(S)-Rg3 (0, 10, 80, 150 µmol/l) and curcumin (0, 10, 30, 50, 90 µg/mL). The inhibitory effect of co-treatment with these herbal drugs with and without 4 Gy radiotherapy on the MDA-MB-231 cell line was examined. Flow cytometry was applied to measure the effect of co-treatment of the drugs on radiation-induced apoptosis. The data were analyzed using ANOVA and Kruskal-Wallis tests. P valuesResults: The results of the MTT assay showed that ginsenoside 20(S)-Rg3 and curcumin had an inhibitory effect on the MDA-MB-231 cell line in a concentration-dependent manner. Ginsenoside 20(S)-Rg3 and curcumin inhibited tumor cell development and proliferation at concentrations of 80 µmol/L and 30 μg/mL, respectively, with 50% cell viability (P=0.018, P=0.01, respectively) at 48 hour incubation time.
Conclusion: Ginsenoside 20(S)-Rg3 and curcumin inhibited MDA-MB-231 cell growth in a dose- and time-dependent manner and increased the radiosensitivity of cancer cells. These herbal drugs can be considered as a radiosensitizer in radiotherapy.
کلیدواژه‌ها
Radiotherapy؛ Curcumin؛ Ginsenoside Rg3
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