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Ji, Yuying, Cao, Rui, Lv, Guangxin, Jin, Yuanyuan, Chen, Jing. (1399). Identification of Functional Fragments in gMYL6 and the Mechanism of Improving NK Cell-Mediated Cytotoxicity. سامانه مدیریت نشریات علمی, 17(4), 292-302. doi: 10.22034/iji.2020.84865.1676
Yuying Ji; Rui Cao; Guangxin Lv; Yuanyuan Jin; Jing Chen. "Identification of Functional Fragments in gMYL6 and the Mechanism of Improving NK Cell-Mediated Cytotoxicity". سامانه مدیریت نشریات علمی, 17, 4, 1399, 292-302. doi: 10.22034/iji.2020.84865.1676
Ji, Yuying, Cao, Rui, Lv, Guangxin, Jin, Yuanyuan, Chen, Jing. (1399). 'Identification of Functional Fragments in gMYL6 and the Mechanism of Improving NK Cell-Mediated Cytotoxicity', سامانه مدیریت نشریات علمی, 17(4), pp. 292-302. doi: 10.22034/iji.2020.84865.1676
Ji, Yuying, Cao, Rui, Lv, Guangxin, Jin, Yuanyuan, Chen, Jing. Identification of Functional Fragments in gMYL6 and the Mechanism of Improving NK Cell-Mediated Cytotoxicity. سامانه مدیریت نشریات علمی, 1399; 17(4): 292-302. doi: 10.22034/iji.2020.84865.1676

Identification of Functional Fragments in gMYL6 and the Mechanism of Improving NK Cell-Mediated Cytotoxicity

Iranian Journal of Immunology
مقاله 4، دوره 17، شماره 4، اسفند 2020، صفحه 292-302    اصل مقاله (541.75 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2020.84865.1676
نویسندگان
Yuying Ji1؛ Rui Cao2؛ Guangxin Lv3؛ Yuanyuan Jin3؛ Jing Chen* 2
1North China University of Science and Technology,Hebei 063210,China
2College of Life Sciences, North China University of Science and Technology, Hebei 063210, China
3Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences, Beijing 100050, China
چکیده
Background: In a previous study, the unrecognized role of gMYL6 in the up-regulation of human NK cells development and cytotoxicity was reported. Objective: To further elucidate the mechanism of action of small recombinant fragments of gMYL6 enhancing the NK cells activity. Methods: Mononuclear cells were isolated from umbilical cord blood (UCB) by density-gradient centrifugation and NK cells were propagated and cultured. The small peptides from the gMYL6, with the ability to enhance the cytotoxicity of NK cells were screened by CCK-8 method and one of the most powerful peptides was identified for the next study. Flow cytometry was used to assess the proliferation and apoptosis of K562 cells, as well as the cell cycle arrest. The apoptosis of target cells was observed by AO/EB fluorescence staining, and the degree of apoptosis was assessed by flow cytometry. Protein imprinting method was also used to explore the pathway of small peptides to enhance the NK cells' activity. On the other hand, Real-time Quantitative PCR Detecting System was used to verify the mechanism of K562 cells suppression. Results: Small D peptide significantly increased NK cells cytotoxicity and induced both cell cycle arrest at G2/M and apoptosis of K562 cells. Conclusion: Small D peptide could be a novel promising peptide for cancer immunotherapy since it was shown to promote the cytotoxicity of cord blood-derived NK cells.
کلیدواژه‌ها
Cancer Immunotherapy؛ gMYL6؛ NK Cell؛ Small Molecule Peptide
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