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Pakniyat, Fatemeh, Mozdarani, Hossein, Nedaee, Hassan Ali, Mahmoudzadeh, Aziz, Salimi, Mahdieh, Gholami, Somayeh. (1401). Bystander Response Following High-Dose X-irradiation; Time-dependent Nature of GammaH2AX Foci and Cell Death Consequences. سامانه مدیریت نشریات علمی, 13(1), 17-28. doi: 10.31661/jbpe.v0i0.2001-1053
Fatemeh Pakniyat; Hossein Mozdarani; Hassan Ali Nedaee; Aziz Mahmoudzadeh; Mahdieh Salimi; Somayeh Gholami. "Bystander Response Following High-Dose X-irradiation; Time-dependent Nature of GammaH2AX Foci and Cell Death Consequences". سامانه مدیریت نشریات علمی, 13, 1, 1401, 17-28. doi: 10.31661/jbpe.v0i0.2001-1053
Pakniyat, Fatemeh, Mozdarani, Hossein, Nedaee, Hassan Ali, Mahmoudzadeh, Aziz, Salimi, Mahdieh, Gholami, Somayeh. (1401). 'Bystander Response Following High-Dose X-irradiation; Time-dependent Nature of GammaH2AX Foci and Cell Death Consequences', سامانه مدیریت نشریات علمی, 13(1), pp. 17-28. doi: 10.31661/jbpe.v0i0.2001-1053
Pakniyat, Fatemeh, Mozdarani, Hossein, Nedaee, Hassan Ali, Mahmoudzadeh, Aziz, Salimi, Mahdieh, Gholami, Somayeh. Bystander Response Following High-Dose X-irradiation; Time-dependent Nature of GammaH2AX Foci and Cell Death Consequences. سامانه مدیریت نشریات علمی, 1401; 13(1): 17-28. doi: 10.31661/jbpe.v0i0.2001-1053

Bystander Response Following High-Dose X-irradiation; Time-dependent Nature of GammaH2AX Foci and Cell Death Consequences

Journal of Biomedical Physics and Engineering
مقاله 3، دوره 13، شماره 1، اردیبهشت 2023، صفحه 17-28    اصل مقاله (3.15 M)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.2001-1053
نویسندگان
Fatemeh Pakniyat1؛ Hossein Mozdarani* 2؛ Hassan Ali Nedaee1، 3؛ Aziz Mahmoudzadeh4؛ Mahdieh Salimi5؛ Somayeh Gholami3
1Department of Medical Physics and Biomedical Engineering, Tehran University of Medical Sciences, Tehran, Iran
2Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3Radiation Oncology Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
4Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran
5Department of Medical Genetics, Medical Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
چکیده
Background: The paradigm shifts in target theory could be defined as the radiation-triggered bystander response in which the radiation deleterious effects occurred in the adjacent cells. 
Objective: This study aims to assess bystander response in terms of DNA damage and their possible cell death consequences following high-dose radiotherapy. Temporal characteristics of gH2AX foci as a manifestation of DNA damage were also evaluated.
Material and Methods: In this experimental study, bystander response was investigated in human carcinoma cells of HeLa and HN5, neighboring those that received high doses. Medium transfer was performed from 10 Gy-irradiated donors to 1.5 Gy-irradiated recipients. GammaH2AX foci, clonogenic and apoptosis assays were investigated. The gH2AX foci time-point study was implemented 1, 4, and 24 h after the medium exchange. 
Results: DNA damage was enhanced in HeLa and HN5 bystander cells with the ratio of 1.27 and 1.72, respectively, which terminated in more than two-fold clonogenic survival decrease, along with gradual apoptosis increase. GammH2AX foci temporal characterization revealed maximum foci scoring at the 1 h time-point in HeLa, and also 4 h in HN5, which remained even 24 h after the medium sharing in higher level than the control group.  
Conclusion: The time-dependent nature of bystander-induced gH2AX foci as a DNA damage surrogate marker was highlighted with the persistent foci at 24 h. considering an outcome of bystander-induced DNA damage, predominant role of clonogenic cell death was also elicited compared to apoptosis. Moreover, the role of high-dose bystander response observed in the current work clarified bystander potential implications in radiotherapy.
کلیدواژه‌ها
Bystander؛ High-Dose؛ GammaH2AX؛ DNA Damage؛ Survival؛ Apoptosis
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