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Mirzaeyan, Fatemeh, Chahardouli, Bahram, Mirzaeian, Amin, Alizad Ghandforoush, Nasrin, Alimoghaddam, Kamran, Rostami, Shahrbano. (1400). Concurrent Evaluation of the Expression and Methylation of Secreted Frizzled-Related Protein 2 along with Beta-Catenin Expression in Patients with non-M3 Acute Myeloid Leukemia. سامانه مدیریت نشریات علمی, 46(3), 180-188. doi: 10.30476/ijms.2020.84316.1396
Fatemeh Mirzaeyan; Bahram Chahardouli; Amin Mirzaeian; Nasrin Alizad Ghandforoush; Kamran Alimoghaddam; Shahrbano Rostami. "Concurrent Evaluation of the Expression and Methylation of Secreted Frizzled-Related Protein 2 along with Beta-Catenin Expression in Patients with non-M3 Acute Myeloid Leukemia". سامانه مدیریت نشریات علمی, 46, 3, 1400, 180-188. doi: 10.30476/ijms.2020.84316.1396
Mirzaeyan, Fatemeh, Chahardouli, Bahram, Mirzaeian, Amin, Alizad Ghandforoush, Nasrin, Alimoghaddam, Kamran, Rostami, Shahrbano. (1400). 'Concurrent Evaluation of the Expression and Methylation of Secreted Frizzled-Related Protein 2 along with Beta-Catenin Expression in Patients with non-M3 Acute Myeloid Leukemia', سامانه مدیریت نشریات علمی, 46(3), pp. 180-188. doi: 10.30476/ijms.2020.84316.1396
Mirzaeyan, Fatemeh, Chahardouli, Bahram, Mirzaeian, Amin, Alizad Ghandforoush, Nasrin, Alimoghaddam, Kamran, Rostami, Shahrbano. Concurrent Evaluation of the Expression and Methylation of Secreted Frizzled-Related Protein 2 along with Beta-Catenin Expression in Patients with non-M3 Acute Myeloid Leukemia. سامانه مدیریت نشریات علمی, 1400; 46(3): 180-188. doi: 10.30476/ijms.2020.84316.1396

Concurrent Evaluation of the Expression and Methylation of Secreted Frizzled-Related Protein 2 along with Beta-Catenin Expression in Patients with non-M3 Acute Myeloid Leukemia

Iranian Journal of Medical Sciences
مقاله 4، دوره 46، شماره 3، مرداد 2021، صفحه 180-188    اصل مقاله (1.04 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2020.84316.1396
نویسندگان
Fatemeh Mirzaeyan1؛ Bahram Chahardouli2؛ Amin Mirzaeian3؛ Nasrin Alizad Ghandforoush2؛ Kamran Alimoghaddam2؛ Shahrbano Rostami* 2
1Department of Hematology and Blood Banking, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran
2Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran
3Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
چکیده
Background: Wnt signaling is a critical pathway for the development of acute myeloid leukemia (AML). Some studies have evaluated the expression or methylation of secreted frizzled-related protein 2 (SFRP2) as an antagonist and beta-catenin (β-catenin) as a critical mediator of this pathway. Since we found no comprehensive study on these genes in Iran, we aimed to investigate the status of both SFRP2 expression and methylation, and also β-catenin expression, in conjunction with clinical characteristics, in Iranian patients with de novo non-M3 AML.
Methods: The methylation and expression of SFRP2 were determined in 188 patients with primary non-M3 AML and 60 healthy controls, who were referred to Shariati Hospital, Tehran, Iran, between January 2017 and February 2019. The methylation-specific polymerase chain reaction (PCR) and real-time quantitative PCR were used, respectively. The expression of β-catenin was explored via real-time quantitative PCR. Statistical analysis was performed using the Mann–Whitney U test (SPSS software, version 23). A P value of less than 0.05 (2-tailed) was considered significant.
Results: SFRP2 mRNA showed a significant decline in the AML group compared with the controls (p <0.001). The hypermethylation of the SFRP2 promoter occurred in 25.5% (48/188) of the cases. SFRP2 expression exhibited a negative correlation with the white blood cell count (P=0.003). The expression of β-catenin increased significantly in the patients in comparison with the controls (p <0001), and a significant difference was observed between the patients, who achieved complete remission and those, who did not (P=0.046).
Conclusion: The findings of this study showed that alterations in SFRP2 and β-catenin expression can be used as a potential biomarker for differentiating patients with new non-M3 AML from the controls. Additionally, an evaluation of β-catenin expression may be valuable in predicting complete remission in patients with non-M3 AML.
کلیدواژه‌ها
SFRP2 protein؛ Humans؛ Leukemia؛ Myeloid؛ Acute؛ beta Catenin؛ Wnt signaling pathway
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