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Alteration of P53, hMLH1, and HER2 Gene in Bangladeshi Gastric Cancer Patients: Their Association with H. pylori Infection and Clinicopathological Factors | ||
| Middle East Journal of Cancer | ||
| مقاله 2، دوره 12، شماره 3 - شماره پیاپی 47، مهر 2021، صفحه 332-341 اصل مقاله (293.19 K) | ||
| نوع مقاله: Original Article(s) | ||
| شناسه دیجیتال (DOI): 10.30476/mejc.2020.84147.1207 | ||
| نویسندگان | ||
| M. Mizanur Rahman* 1؛ Gazi Nurun Nahar Sultana2؛ Mohammed Abu Kawsar Sarker3؛ Md. Monoar Hossain4؛ Mohd. Sahajadul Alam1؛ Md. Anisur Rahman5؛ Abdul Ahad6؛ Md. Monzurul Islam1؛ Rokeya Begum2 | ||
| 1National Institute of Cancer Research and Hospital, Mohakhali, Dhaka, Bangladesh | ||
| 2Genetic Engineering and Biotechnology Research, Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh | ||
| 3Department of General and Colorectal Surgery, Furness General Hospital, University Hospitals of Morecamb NHS Trust, Barrow-in-Furness, Lancaster, U.K. | ||
| 4Department of Surgical Oncology, Khulna Medical College, Khulna, Bangladesh | ||
| 5Department of Gastroenterology, Popular Medical College Hospital, Dhanmondi, Dhaka, Bangladesh | ||
| 6Department of Gastroenterology, Khulna Medical College, Khulna, Bangladesh | ||
| چکیده | ||
| Background: Gene alterations are required for the development of gastric cancer, which are influenced by environmental and host factors. We conducted the present study to find the status of Helicobacter pylori (H. pylori) infection and its association with altered genes P53, hMLH1, and HER2 in gastric cancer patients and to analyze their correlation with clinical, pathological, and environmental factors. Method: This was a cross-sectional study. For genetic (P53 and hMLH1) study of the gastrectomized tissue DNA extraction and optimization, we performed PCR amplification and DNA sequencing. HER2 was studied by immunochemical technique. The results were matched with tumor status, age and sex, smoking, and H. pylori antibody status of the patients to find their association. Results: The mean age of the patients was 52.91 (±13.94) years. Among the 45 patients selected for genetic tests, 12 aged 40 or more and 33 aged over 40. Among the genes, 33(73.3%) in P53 and 17(37.7%) in hMLH1 were mutated and 11(24.2%) in HER2 were found to be overexpressed. Chi square and regression analysis showed that they all had associations with H. pylori positivity (P < 0.05, odds ratio >1). hMLH1 was associated with the location of the tumor, smoking, sex, blood group, and age, and P53 was found to be affected by extra salt intake, sex, blood group, and age of the patients (P ≤ 0.05 ). Conclusion: Genetic mutation was found in nearly all the patients with gastric cancer, which was significantly associated with H. pylori infection. Mass eradication of this organism might play a role in reducing cancer incidence in Bangladesh. | ||
| کلیدواژه‌ها | ||
| Stomach neoplasms؛ Helicobacter pylori؛ Genes؛ P53؛ hMLH1؛ HER2 | ||
| اصل مقاله | ||
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How to cite this article: Rahman MM, Nahar Sultana GN, Sarker M, Hossain M, Sahajadul Alam M, Rahman A, et al. Alteration of P53, hMLH1, and HER2 gene in Bangladeshi gastric cancer patients: Their association with H. pylori infection and clinicopathological factors. Middle East J Cancer. 2021;12(3):332-41. doi: 10.30476/mejc.2020.84147.1207. | ||
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