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Armeniacae Semen Regulates Apoptosis and Cell Cycle Progression in MCF-7, MDA-MB-231, and T47D Breast Cancer Cell Lines | ||
Middle East Journal of Cancer | ||
مقاله 6، دوره 12، شماره 2 - شماره پیاپی 46، تیر 2021، صفحه 208-218 اصل مقاله (3 M) | ||
نوع مقاله: Original Article(s) | ||
شناسه دیجیتال (DOI): 10.30476/mejc.2020.83374.1159 | ||
نویسندگان | ||
Shiva Mosadegh Manshadi1؛ Fatemeh Nadali1؛ Mohammad Reza Shams Ardekani* 2 | ||
1Department of Hematology and Blood Banking, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran | ||
2Department of Pharmacognosy and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran | ||
چکیده | ||
Background: Amygdalin is a glycoside ingredient of rosacea plants that exerts an antitumor effect by blocking the growth of the tumor cells. Therefore, we aimed to use the Armeniacae semen, a member of the Rosacea family, which contains a large amount of the amygdalin, to evaluate its antiproliferative effect on MCF-7, MDA-MB-231, and T47D breast cancer cell lines. Method: In this experimental study, we prepared the aqueous, ethyl acetate, and hydro-alcoholic extracts of the Armeniacae semen. The MCF-7, MDA-MB-231, and T47D cell lines were treated with different doses of the extracts for 12, 24, 36, and 48 hours; cell viability was investigated with MTT test and cell apoptosis was detected by use of double staining fluorescent. Cell cycle progression was analyzed using a BD Cycle TEST PLUS DNA Kit. We also assessed Bcl2, Bax, and caspase-3 mRNA expression. Results: The best IC50s belonged to hydro-alcoholic extract of the Armeniacae semen in all three cell lines for the 48-hour treatment. We observed a significant increase in Bax and caspase-3 mRNA expression and a noticeable reduction in Bcl2 mRNA compared with the controls. Application of amygdalin to MCF-7, MDA-MB-231, and T47D cell lines increased the number of G0/G1 cells and reduced the number of cells at G2/M phasecompared to the controls. Conclusion: This study showed that the hydro-alcoholic extract of Prunus armeniaca had antitumor effects on breast cancer cell lines as it inhibited the cell cycle at G0/G1 phase and apoptosis induction in the MCF-7, MDA-MB-231, and T47D cell lines. | ||
کلیدواژهها | ||
Armeniacae semen؛ Breast neoplasms؛ Cell cycle؛ Caspase 3؛ Proto-oncogene proteins c-bcl-2؛ Bax | ||
اصل مقاله | ||
How to cite this article: Mosadegh Manshadi S, Nadali F, Shams Ardekani M. Armeniacae semen regulates apoptosis and cell cycle progression in MCF-7, MDA-MB-231, and T47D breast cancer cell lines. Middle East J Cancer. 2021;12(2):208-18. doi: z0.30476/mejc.2020.83374.1159. | ||
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