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Evaluation of miR-107, DAPK1, and KLF4 Expression in Colorectal Tumors and Effect of Oxaliplatin and 5-FU on their Levels in Colorectal Cancer Cell Lines | ||
| Middle East Journal of Cancer | ||
| مقاله 4، دوره 12، شماره 2 - شماره پیاپی 46، تیر 2021، صفحه 190-197 اصل مقاله (1.4 M) | ||
| نوع مقاله: Original Article(s) | ||
| شناسه دیجیتال (DOI): 10.30476/mejc.2020.83091.1131 | ||
| نویسندگان | ||
| Sahar Safaei1؛ Dariush Shanehbandi1؛ Venus Zafari2؛ Elham Eghbali3؛ Mahsa Sadeghzadeh2؛ Haniye Mohammad Reza Khani1؛ Amin Sadrazar4؛ Masood Faghihdinevari4؛ Masoud Shirmohamadi* 4 | ||
| 1Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 2Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 3Medical Radiation Sciences Research Group, Tabriz University of Medical Sciences, Tabriz, East-Azarbaijan, Iran | ||
| 4Gastroenterology and Hepatology Department, Liver and Gastrointestinal Diseases Research Center, Tabriz, Iran | ||
| چکیده | ||
| Background: In recent years, the role of micro-RNAs in the cancer pathophysiology has attracted a great deal of scientific attention. MiRNAs regulate a variety of cellular functions, such as apoptosis, differentiation and migration by targeting oncogenic or tumor suppressor genes. We conducted the current study to assess the expression of miR-107, Krüppel-like factor 4 (KLF4) and death-associated protein kinase (DAPK1) genes in malignant and normal colon tissues and also colorectal cancer (CRC) model cells exposed to oxaliplatin and 5-FU chemotherapy agents. Method: In this case-control study, the tissue samples from CRC patients were collected during colonoscopy process in 2013 -2016 at Imam Reza hospital. Subsequently, the expression levels of miR-107, KLF4, and DAPK1 were detected with quantitative Real-Time PCR. Furthermore, in the in vitro phase of this study, we investigated the changes in the expression level of miR-107, KLF4 and DAPK1 transcripts after oxaliplatin and 5-FU treatment. Results: Unlike miR-107, the expression levels of KLF4 and DAPK1 genes decreased in the tumor samples compared to those in the marginal specimens. In addition, both oxaliplatin and 5-FU significantly increased the expression level of miR-107. There were significant correlations between the expression levels of miR-107, KLF4, and DAPK1genes and clinicopathological features, for instance lymph node metastasis and cell differentiation. Conclusion: The current study suggested a tumor suppressor role for KLF4 and DAPK1 in CRC. The altered expression of miR-107, KLF-4, and DAPK1 genes in CRC tumors and healthy tissues could be utilized for CRC diagnosis and prognosis. Furthermore, the studied genes could be considered as potential therapeutic targets in CRC. | ||
| کلیدواژه‌ها | ||
| Colorectal neoplasms؛ miR-107؛ KLF4؛ DAPK1؛ Oxaliplatin؛ 5-FU | ||
| اصل مقاله | ||
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How to cite this article: Safaei S, Shanehbandi D, Zafari V, Eghbali E, Sadeghzadeh M, Mohammad Reza Khani H, et al. Evaluation of miR-107, DAPK1 and KLF4 expression in colorectal tumors and effect of oxaliplatin and 5-FU on their levels in colorectal cancer cell lines. Middle East J Cancer. 2021; 12(2): 190-7. doi: 10.30476/mejc.2020.83091.1131.
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