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Preliminary study for the preparation of fatty acid bioconjugated L-asparaginase micellar nanocarrier as a delivery system for peptide anti-cancer agents | ||
| Trends in Pharmaceutical Sciences | ||
| مقاله 4، دوره 6، شماره 3، آذر 2020، صفحه 175-188 اصل مقاله (579.55 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/tips.2020.87871.1066 | ||
| نویسندگان | ||
| Hajar Ashrafi* 1؛ amir Azadi2؛ Soliman Mohammadi-Samani3؛ Younes Ghasemi4؛ Saeid** Daneshamouz5 | ||
| 1Department of pharmaceutics, school of pharmacy, Shiraz university of medical sciences, Shiraz, Iran | ||
| 2Department of Pharmaceutics, school of Pharmacy, Siraz University of Medical Sciences, Shiraz, Iran | ||
| 3Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| 4Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 5Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| In the present study, the mixing behavior of different groups of surfactants and fatty acid bioconjugated L-asparaginase investigated. The amphiphilic macromolecules were achieved by covalent linkage of fatty acids with different chain lengths (C12, C16, and C22) to the native enzyme, L-asparaginase. The amino group of L-asparaginase lysine residue was conjugated to the carboxylic group of fatty acids, using a carbodiimide activator. The effect of different kinds of surfactants on particle size and enzyme activity of fatty acid bioconjugated L-ASNase was evaluated. The particle sizes of resulted micellar nanocarrier before and after lyophilization and enzyme activity investigated. Among all surfactants, pluronic F-127 with fatty acid bioconjugated L-asparaginase presented more plasma and PBS half-life. Higher activity value after lyophilization for bioconjugate with pluronic F-127 also achieved. These findings from L-asparaginase modification by fatty acid and surfactants indicate a promising stabilized product that may serve as a new candidate for medical purposes. | ||
| کلیدواژه‌ها | ||
| L-asparaginase؛ Lipid-protein conjugation؛ micellar nanocarrier؛ Pluronic F-127 | ||
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