پیوندهای مفید
آمار
| تعداد نشریات | 20 |
| تعداد شماره‌ها | 1,149 |
| تعداد مقالات | 10,519 |
| تعداد مشاهده مقاله | 45,426,049 |
| تعداد دریافت فایل اصل مقاله | 11,296,805 |
Sensitivity of Four Various Candida Species to Photodynamic Therapy Mediated by Indocyanine Green, an in vitro Study | ||
| Journal of Dentistry | ||
| مقاله 7، دوره 22، شماره 2 - شماره پیاپی 71، شهریور 2021، صفحه 118-124 اصل مقاله (448.96 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/dentjods.2020.81817.0 | ||
| نویسندگان | ||
| Atefeh Tavangar1؛ Faezeh Khozaymeh1؛ Mahdi Razzaghi-Abyaneh2؛ Safieh Sherkat* 3 | ||
| 1Dental Research Center, Dept. of Oral Medicine, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran. | ||
| 2Dept. of Mycology, Pasteur Institute of Iran, Tehran, Iran. | ||
| 3Dept. of Oral Medicine of Shahrekord University of Medical Sciences, Shahrekord, Iran. | ||
| چکیده | ||
| Statement of the Problem: Various species of candida contribute to oral candidiasis. It is the time to shift from conventional rigid antimicrobial therapies to more patient specific and safer ones. Purpose: The present study aimed to investigate antifungal effects of photodynamic therapy (PDT) using Indocyanine green as photosensitizer and low-power laser irradiation on the viability of candida albicans, candida tropicalis, candida glabrata and candida krusei, and to compare it with Nystatin as the conventional treatment. Materials and Method: In this in vitro study, 0.5 McFarland suspensions of candida's species were prepared (n=50, each). Each strain was then divided into five groups of 10 samples each, according to the following experimental interventions: (1) Nystatin, (2) photodynamic therapy: laser irradiation (wavelength= 808 nm, power= 100 mW, energy density= 10 J/cm2, exposure duration= 100 s) in the presence of the photosensitizer, (3) laser irradiation alone, (4) treatment with the PS alone and (5-control: no exposure to laser light or photosensitizer. Next, serial dilutions were prepared and seeded onto Sabouraud dextrose agar. The colonies were counted, and the values of log (CFU/ml) were analyzed by variance and the Tamhan test (p < 0.05). Results: Photodynamic therapy mediated indocyanine green is significantly effective in reducing the number of CFU/ml of all species of candida tested, compared to control group (p < .001). Nystatin, laser irradiation and photodynamic therapy, with respectively decreasing potency, have considerably reduced the number of candida's colonies in all four bacterial strains (p candida albicans, in comparison to control group. Conclusion: It seems that laser therapy alone is more powerful than photodynamic therapy mediated indocyanine green; however, conventional treatment has still the top antimicrobial efficacy towards all candida species. | ||
| کلیدواژه‌ها | ||
| Candida؛ Indocyanine green؛ Laser؛ Nystatin؛ Photodynamic therapy؛ Photosensitizer | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
[1] Azizi A, Rezaei M. Prevalence of Candida species in the oral cavity of patients undergoing head and neck radiotherapy. Journal of Dent Res. Dental Clinics, Dental Prospects. 2009; 3: 78. [2] Farah CS, Ashman RB, Challacombe SJ. Oral candidosis. Clinics in Dermatology. 2000; 18: 553-562. [3] Ferretti GA, Raybould TP, Brown AT, Macdonald JS, Greenwood M, Maruyama Y, et al. Chlorhexidine prophylaxis for chemotherapy-and radiotherapy-induced stomatitis: a randomized double-blind trial. Oral Surgery, Oral Medicine, Oral Pathology. 1990; 69: 331-338. [4] Johnson EM, Warnock DW, Luker J, Porter SR, Scully C. Emergence of azole drug resistance in Candida species from HIV-infected patients receivingprolonged fluconazole therapy for oral candidosis. Journal of Antimicrobial Chemotherapy. 1995; 35: 103-114. [5] Worthington HV, Clarkson JE, Khalid T, Meyer S, McCabe M. Interventions for treating oral candidiasis for patients with cancer receiving treatment. Available at: https://www.cochrane.org/CD001972/ORAL_interventions-for-treating-oral-candidiasis-for-patients-with-cancer-receiving-treatment [6] Cowen LE, Singh SD, Köhler JR, Collins C, Zaas AK, Schell WA, et al. Harnessing Hsp90 function as a powerful, broadly effective therapeutic strategy for fungal infectious disease. Proceedings of the National Academy of Sciences. 2009; 106: 2818-2823. [7] Ghalyani P, Karami M, Havaei A, Naderi A, Alikhani M. Contamination of Dental Scaler Waterlines with Legionella Pneumophila, Pseudomonas Aeruginosa and Gram Positive Cocci. Journal of Islamic Dental Association of Iran. 2015; 27: 53-57. [8] Chabrier‐Roselló Y, Foster TH, Mitra S, Haidaris CG. Respiratory deficiency enhances the sensitivity of the pathogenic fungus Candida to photodynamic treatment. Photochemistry and photobiology. 2008; 84: 1141-1148. [9] Garcez AS, Ribeiro MS, Tegos GP, Núñez SC, Jorge AO, Hamblin MR. Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection. Lasers in Surgery and Medicine. 2007; 39: 59-66. [10] Urbanska K, Romanowska-Dixon B, Matuszak Z, Oszajca J, Nowak-Sliwinska P, Stochel G. Indocyanine green as a prospective sensitizer for photodynamic therapy of melanomas. Acta Biochimica Polonica. 2002; 49: 387-391. [11] Parker S. The use of diffuse laser photonic energy and indocyanine green photosensitiser as an adjunct to periodontal therapy. British Dental Journal. 2013; 215: 167-171. [12] Takasaki AA, Aoki A, Mizutani K, Schwarz F, Sculean A, Wang CY, et al. Application of antimicrobial photodynamic therapy in periodontal and peri‐implant diseases. Periodontology 2000. 2009; 51: 109-140. [13] Fekrazad R, Ghasemi*Barghi V, Poorsattar*Bejeh Mir A, Shams-Ghahfarokhi M. In vitro photodynamic inactivation of Candida albicans by phenothiazine dye (new methylene blue) and Indocyanine green (EmunDo). Photodiagnosis and Photodynamic Therapy. 2015; 12: 52-57. [14] Azizi A, Amirzadeh Z, Rezai M, Lawaf S, Rahimi A. Effect of photodynamic therapy with two photosensitizers on Candida albicans. Journal of Photochemistry and Photobiology B: Biology. 2016; 158: 267-273. [15] Souza RC, Junqueira JC, Rossoni RD, Pereira CA, Munin E, Jorge AO. Comparison of the photodynamic fungicidal efficacy of methylene blue, toluidine blue, malachite green and low-power laser irradiation alone against Candida albicans. Lasers in Medical Science. 2010; 25: 385-389. [16] Baltazar LM, Ray A, Santos DA, Cisalpino PS, Friedman AJ, Nosanchuk JD. Antimicrobial photodynamic therapy: an effective alternative approach to control fungal infections. Frontiers in Microbiology. 2015; 6: 202. [17] Shi H, Li J, Zhang H, Zhang J, Sun H. Effect of 5-aminolevulinic acid photodynamic therapy on Candida albicans biofilms: An in vitro study. Photodiagnosis and Photodynamic Ttherapy. 2016; 15: 40-45. [18] Bliss JM, Bigelow CE, Foster TH, Haidaris CG. Susceptibility of Candida species to photodynamic effects of photofrin. Antimicrobial Agents and Chemotherapy. 2004; 48: 2000-2006. [19] Wilson M, Mia N. Sensitisation of Candida albicans to killing by low‐power laser light. Journal of Oral Pathology & Medicine. 1993; 22: 354-357. [20] Codling CE, Maillard JY, Russell AD. Aspects of the antimicrobial mechanisms of action of a polyquaternium and an amidoamine. Journal of Antimicrobial Chemotherapy. 2003; 51: 1153-1158. [21] Konopka K, Goslinski T. Photodynamic therapy in dentistry. Journal of Dent Res. 2007; 86: 694-707. [22] Bonsor S, Nichol R, Reid T, Pearson G. Microbiological evaluation of photo-activated disinfection in endodontics (an in vivo study). British Dental Journal. 2006; 200: 337. [23] Freire F, Ferraresi C, Jorge AOC, Hamblin MR. Photodynamic therapy of oral Candida infection in a mouse model. Journal of Photochemistry and Photobiology Biology. 2016; 159: 161-168. [24] Schoop U, Kluger W, Moritz A, Nedjelik N, Georgopoulos A, Sperr W. Bactericidal effect of different laser systems in the deep layers of dentin. Lasers in Surgery and Medicine. 2004; 35: 111-116. [25] Silva EJ, Coutinho-Filho WP, Andrade AO, Herrera DR, Coutinho-Filho TS, Krebs RL. Evaluation of photodynamic therapy using a diode laser and different photosensitizers against enterococcus faecalis. Acta Odontológica Latinoamericana. 2014; 27: 63-65. [26] Hirata S, Kitamura C, Fukushima H, Nakamichi I, Abiko Y, Terashita M, et al. Low‐level laser irradiation enhances BMP‐induced osteoblast differentiation by stimulating the BMP/Smad signaling pathway. Journal of Cellular Biochemistry. 2010; 111: 1445-1452. [27] Wainwright M. Photodynamic antimicrobial chemotherapy (PACT). The Journal of Antimicrobial Chemotherapy. 1998; 42: 13-28. [28] Korytnicki D, Mayer MPA, Daronch M, Singer JdM, Grande RHM. Effects of Nd: YAG laser on enamel microhardness and dental plaque composition: an in situ study. Photomedicine and Laser Surgery. 2006; 24: 59-63. [29] Müller P, Guggenheim B, Schmidlin PR. Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. European Journal of Oral Sciences. 2007; 115: 77-80. [30] Tuchin VV, Genina EA, Bashkatov AN, Simonenko GV, Odoevskaya OD, Altshuler GB. A pilot study of ICG laser therapy of acne vulgaris: photodynamic and photothermolysis treatment. Lasers in Surgery and Medicine. 2003; 33: 296-310. [31] McCorquodale EM, Colyer CL. Indocyanine green as a noncovalent, pseudofluorogenic label for protein determination by capillary electrophoresis. Electrophoresis. 2001; 22: 2403-2408. | ||
|
آمار تعداد مشاهده مقاله: 3,384 تعداد دریافت فایل اصل مقاله: 2,430 |
||
