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Iman Islamieh, Davoud, Goudarzi, Hossein, Khaledi, Azad, Afshar, Davoud, Esmaeili, Davoud. (1399). Reduced Efflux Pumps Expression of Pseudomonas Aeruginosa with Satureja Khuzistanica Essential Oil. سامانه مدیریت نشریات علمی, 45(6), 463-468. doi: 10.30476/ijms.2019.72675.
Davoud Iman Islamieh; Hossein Goudarzi; Azad Khaledi; Davoud Afshar; Davoud Esmaeili. "Reduced Efflux Pumps Expression of Pseudomonas Aeruginosa with Satureja Khuzistanica Essential Oil". سامانه مدیریت نشریات علمی, 45, 6, 1399, 463-468. doi: 10.30476/ijms.2019.72675.
Iman Islamieh, Davoud, Goudarzi, Hossein, Khaledi, Azad, Afshar, Davoud, Esmaeili, Davoud. (1399). 'Reduced Efflux Pumps Expression of Pseudomonas Aeruginosa with Satureja Khuzistanica Essential Oil', سامانه مدیریت نشریات علمی, 45(6), pp. 463-468. doi: 10.30476/ijms.2019.72675.
Iman Islamieh, Davoud, Goudarzi, Hossein, Khaledi, Azad, Afshar, Davoud, Esmaeili, Davoud. Reduced Efflux Pumps Expression of Pseudomonas Aeruginosa with Satureja Khuzistanica Essential Oil. سامانه مدیریت نشریات علمی, 1399; 45(6): 463-468. doi: 10.30476/ijms.2019.72675.

Reduced Efflux Pumps Expression of Pseudomonas Aeruginosa with Satureja Khuzistanica Essential Oil

Iranian Journal of Medical Sciences
مقاله 7، دوره 45، شماره 6، بهمن 2020، صفحه 463-468    اصل مقاله (344.37 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2019.72675.
نویسندگان
Davoud Iman Islamieh1، 2؛ Hossein Goudarzi2؛ Azad Khaledi3، 4؛ Davoud Afshar5؛ Davoud Esmaeili* 1، 6
1Applied Microbiology Research Center, Department of Microbiology, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
2Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Infectious Diseases Research Center, Kashan University of Medical Science, Kashan, Iran
4Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Science, Kashan, Iran
5Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
6Applied Virology Research Center, Baqiyatallah University of Medical sciences, Tehran, Iran
چکیده
Background: Efflux pumps such as MexEF-OprN and mexXY-OprM play an important role in the resistance of Pseudomonas aeruginosa (P. aeruginosa) to antibiotics. The present study aimed to assess the reduced expression of efflux pump genes of P. aeruginosa with Satureja khuzistanica essential oil (SKEO).
Methods: The present cross-sectional study was conducted in 2016 at the Microbiology Laboratory of Baqiyatallah University of Medical Sciences, Tehran, Iran. The disk diffusion method was used for susceptibility testing of gentamicin and norfloxacin. Minimum inhibitory concentration (MIC) was determined for gentamicin and norfloxacin. The antibacterial efficacy of SKEO was defined by determining the MIC values using the microdilution method. In vitro, the synergistic interaction of SKEO combined with gentamicin or norfloxacin was examined via checkerboard assay and defined as a fractional inhibitory concentration index. The reverse transcription-polymerase chain reaction technique was used to measure changes in the expression of the efflux pump genes. The data were analyzed using SPSS software version 16.0, and p Results: The MIC values of SKEO were in the range of 6 to 12 µg/mL. In the presence of sub-inhibitory concentrations (1.16 to 2 MIC) of SKEO, synergistic effects were revealed using the checkerboard method. The effect of norfloxacin and gentamicin increased up to 8-fold. The expression of mexY and mexE was reduced after treatment with SKEO.
Conclusion: SKEO reduced the expression of efflux pumps and the MIC values of norfloxacin and gentamicin in vitro.
کلیدواژه‌ها
Efflux pump؛ Gene expression؛ Pseudomonas aeruginosa؛ Norfloxacin؛ Gentamicin
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مراجع
  1. Breidenstein EB, de la Fuente-Nunez C, Hancock RE. Pseudomonas aeruginosa: all roads lead to resistance. Trends Microbiol. 2011;19:419-26. doi: 10.1016/j.tim.2011.04.005. PubMed PMID: 21664819.
  2. Zhanel GG, Hoban DJ, Schurek K, Karlowsky JA. Role of efflux mechanisms on fluoroquinolone resistance in Streptococcus pneumoniae and Pseudomonas aeruginosa. Int J Antimicrob Agents. 2004;24:529-35. doi: 10.1016/j.ijantimicag.2004.08.003. PubMed PMID: 15555873.
  3. Iman Islamieh D, Afshar D, Yousefi M, Esmaeili D. Efflux pump inhibitors derived from natural sources as novel antibacterial agents against Pseudomonas aeruginosa: a review. Int J Med Rev. 2018;5:94-105. doi: 10.29252/IJMR-050303.
  4. Avrain L, Mertens P, Van Bambeke F. RND efflux pumps in P. aeruginosa: an underestimated resistance mechanism. Antibiot Susceptibility. 2013;26321:26-8.
  5. Masuda N, Sakagawa E, Ohya S, Gotoh N, Tsujimoto H, Nishino T. Substrate specificities of MexAB-OprM, MexCD-OprJ, and MexXY-oprM efflux pumps in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2000;44:3322-7. doi: 10.1128/aac.44.12.3322-3327.2000. PubMed PMID: 11083635; PubMed Central PMCID: PMCPMC90200.
  6. Hirakata Y, Kondo A, Hoshino K, Yano H, Arai K, Hirotani A, et al. Efflux pump inhibitors reduce the invasiveness of Pseudomonas aeruginosa. Int J Antimicrob Agents. 2009;34:343-6. doi: 10.1016/j.ijantimicag.2009.06.007. PubMed PMID: 19615866.
  7. Llanes C, Hocquet D, Vogne C, Benali-Baitich D, Neuwirth C, Plesiat P. Clinical strains of Pseudomonas aeruginosa overproducing MexAB-OprM and MexXY efflux pumps simultaneously. Antimicrob Agents Chemother. 2004;48:1797-802. doi: 10.1128/aac.48.5.1797-1802.2004. PubMed PMID: 15105137; PubMed Central PMCID: PMCPMC400543..
  8. Morita Y, Murata T, Mima T, Shiota S, Kuroda T, Mizushima T, et al. Induction of mexCD-oprJ operon for a multidrug efflux pump by disinfectants in wild-type Pseudomonas aeruginosa PAO1. J Antimicrob Chemother. 2003;51:991-4. doi: 10.1093/jac/dkg173. PubMed PMID: 12654738.
  9. Llanes C, Kohler T, Patry I, Dehecq B, van Delden C, Plesiat P. Role of the MexEF-OprN efflux system in low-level resistance of Pseudomonas aeruginosa to ciprofloxacin. Antimicrob Agents Chemother. 2011;55:5676-84. doi: 10.1128/AAC.00101-11. PubMed PMID: 21911574; PubMed Central PMCID: PMCPMC3232816.
  10. Stavri M, Piddock LJ, Gibbons S. Bacterial efflux pump inhibitors from natural sources. J Antimicrob Chemother. 2007;59:1247-60. doi: 10.1093/jac/dkl460. PubMed PMID: 17145734.
  11. Chitemerere TA, Mukanganyama S. In vitro antibacterial activity of selected medicinal plants from Zimbabwe. The African Journal of Plant Science and Biotechnology. 2011;5:1-7.
  12. Ricci V, Busby SJ, Piddock LJ. Regulation of RamA by RamR in Salmonella enterica serovar Typhimurium: isolation of a RamR superrepressor. Antimicrob Agents Chemother. 2012;56:6037-40. doi: 10.1128/AAC.01320-12. PubMed PMID: 22948865; PubMed Central PMCID: PMCPMC3486581.
  13. Espy MJ, Uhl JR, Sloan LM, Buckwalter SP, Jones MF, Vetter EA, et al. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev. 2006;19:165-256. doi: 10.1128/CMR.19.1.165-256.2006. PubMed PMID: 16418529; PubMed Central PMCID: PMCPMC1360278.
  14. Seghatoleslami S, Samadi N, Salehnia A, Azimi S. Antibacterial activity of endemic Satureja Khuzistanica Jamzad essential oil against oral pathogens. Iran Endod J. 2009;4:5-9. PubMed PMID: 23864870; PubMed Central PMCID: PMCPMC3712262.
  15. Saghi H, Bahador A, Khaledi A, ATAEE KR, AMIRI DF, Esmaeili D. Antibacterial effects of Origanum vulgare essence against multidrug-resistant Acinetobacter baumannii isolated from selected hospitals of Tehran, Iran. Avicenna J Clin Microb Infec. 2015;2:e22982. doi: 10.17795/ajcmi-22982.
  16. Jain D, Jayaram L, Prabhu VM, Bhat GK. Antibacterial effect of neem (Azadirachta indica) oil on multidrug resistant bacteria isolated from human infections. International Journal of Biological & Medical Research. 2013;4:3544-6.
  17. Jalalvandi¹ N, Bahador A, Zahedi B, Saghi H, Esmaeili D. The study of inhibitory effects of satureja khuzestanica essence against mexa and mexr efflux genes ofpseudomonas aeruginosa by rt-pcr. International Journal of Biotechnology. 2015;4:1-8. doi: 10.18488/journal.57/2015.4.1/57.1.1.8.
  18. 18. Sopirala MM, Mangino JE, Gebreyes WA, Biller B, Bannerman T, Balada-Llasat JM, et al. Synergy testing by Etest, microdilution checkerboard, and time-kill methods for pan-drug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother. 2010;54:4678-83. doi: 10.1128/AAC.00497-10. PubMed PMID: 20713678; PubMed Central PMCID: PMCPMC2976112.
  19. Stefanovic O, Stankovic MS, Comic L. In vitro antibacterial efficacy of Clinopodium vulgare L. extracts and their synergistic interaction with antibiotics. Journal of Medicinal Plants Research. 2011;5:4074-9.
  20. Li XZ, Plesiat P, Nikaido H. The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria. Clin Microbiol Rev. 2015;28:337-418. doi: 10.1128/CMR.00117-14. PubMed PMID: 25788514; PubMed Central PMCID: PMCPMC4402952.
  21. Bahador A, Saghii H, Ataee R, Esmaeili D. The Study of inhibition effects Satureja khuzestaniea essence against gene expression bap acinetobacter baumannii with real time PCR technique. Iranian Journal of Medical Microbiology. 2015;9:42-9.
  22. Esmaeili D, Moradi F, Foladi AI, Bahador A. The Study of Inhibitory Effects of Satureja khuzestanicaEssence against Exos Gene of MDR Pseudomonas aeruginosa by RT-PCR Technique. Pharmaceut Reg Affairs. 2015;4. doi: 10.4172/2167-7689.1000151.
  23. Islamieh DI, Afshar D, Esmaeili D. Effect of Satureja khuzistanica essential oil (SKEO) extract on expression of lasA and lasB genes in Pseudomonas aeruginosa. Iran J Microbiol. 2019;11:55-9. PubMed PMID: 30996832; PubMed Central PMCID: PMCPMC6462270.
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