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Yabr Lafta, Haider, Fallahi, Hossein, Yari, Kheirollah. (1399). Detection of an Insertion in the ATXN3 Gene in Chronic Myeloid Leukemia Cases Using Exome Sequencing. سامانه مدیریت نشریات علمی, 12(1), 143-146. doi: 10.30476/mejc.2020.81911.1051
Haider Yabr Lafta; Hossein Fallahi; Kheirollah Yari. "Detection of an Insertion in the ATXN3 Gene in Chronic Myeloid Leukemia Cases Using Exome Sequencing". سامانه مدیریت نشریات علمی, 12, 1, 1399, 143-146. doi: 10.30476/mejc.2020.81911.1051
Yabr Lafta, Haider, Fallahi, Hossein, Yari, Kheirollah. (1399). 'Detection of an Insertion in the ATXN3 Gene in Chronic Myeloid Leukemia Cases Using Exome Sequencing', سامانه مدیریت نشریات علمی, 12(1), pp. 143-146. doi: 10.30476/mejc.2020.81911.1051
Yabr Lafta, Haider, Fallahi, Hossein, Yari, Kheirollah. Detection of an Insertion in the ATXN3 Gene in Chronic Myeloid Leukemia Cases Using Exome Sequencing. سامانه مدیریت نشریات علمی, 1399; 12(1): 143-146. doi: 10.30476/mejc.2020.81911.1051

Detection of an Insertion in the ATXN3 Gene in Chronic Myeloid Leukemia Cases Using Exome Sequencing

Middle East Journal of Cancer
مقاله 46، دوره 12، شماره 1 - شماره پیاپی 45، فروردین 2021، صفحه 143-146    اصل مقاله (742.28 K)
نوع مقاله: Brief Report(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2020.81911.1051
نویسندگان
Haider Yabr Lafta1، 2؛ Hossein Fallahi* 1؛ Kheirollah Yari3
1Department of Biology, School of Sciences, Razi University, Kermanshah, Iran
2Central Laboratories, Ministry of Science and Technology, Baghdad, Iraq
3Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
چکیده
Background: Chronic myeloid leukemia (CML) is a common type of cancer. Leukemia is associated with diverse molecular and genetic changes, including loss and gain of chromosomes, gene deletions, duplications, point mutations, and gene fusions derived from chromosomal translocations. Advanced genetic tests are done to diagnose leukemia; however, many patients are not diagnosed because many of the symptoms are vague, unspecific, and referable to other diseases.
Method: Following the extraction of genomic DNA from CML patient, we performed exome sequencing. Variants were detected via GATK software. Novel alterations in CML cases were then visualized in the integrated genome browser. To enrich our findings, we included exome sequencing data pertaining to 11 individuals. The data was deposited in the ENA database in our analysis. Afterwards, we verified insertion in the ATXN3 gene through performing PCR reactions for both healthy and CML cases.
Results: We identified an alteration in the genomic sequence of the ATXN3 gene in the CML cases.
Conclusion: A correlation existed between insertion in the ATXN3 gene and positive CML cases. These findings might be conducive to the detection of CML at the early stages of the disease.
کلیدواژه‌ها
Chronic myeloid leukemia؛ Exome sequencing؛ ATXN3
اصل مقاله

How to cite this article:

Lafta HY, Fallahi H, Yari K. Detection of an insertion in the ATXN3 gene in chronic myeloid leukemia cases using exome sequencing. Middle East J Cancer. 2021;12(1):143-6. doi: 10.30476/mejc.2020.81911.1051.

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