LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,089
تعداد مقالات9,995
تعداد مشاهده مقاله26,317,310
تعداد دریافت فایل اصل مقاله7,270,246
Taeb, Shahram, Mosleh-Shiraz, Mohammad Amin, Ghaderi, Abbas, Mortazavi, Seyed Mohammad Javad, Razmkhah, Mahboobeh. (1400). Adipose-Derived Mesenchymal Stem Cells Responses to Different Doses of Gamma Radiation. سامانه مدیریت نشریات علمی, 12(1), 35-42. doi: 10.31661/jbpe.v0i0.1212
Shahram Taeb; Mohammad Amin Mosleh-Shiraz; Abbas Ghaderi; Seyed Mohammad Javad Mortazavi; Mahboobeh Razmkhah. "Adipose-Derived Mesenchymal Stem Cells Responses to Different Doses of Gamma Radiation". سامانه مدیریت نشریات علمی, 12, 1, 1400, 35-42. doi: 10.31661/jbpe.v0i0.1212
Taeb, Shahram, Mosleh-Shiraz, Mohammad Amin, Ghaderi, Abbas, Mortazavi, Seyed Mohammad Javad, Razmkhah, Mahboobeh. (1400). 'Adipose-Derived Mesenchymal Stem Cells Responses to Different Doses of Gamma Radiation', سامانه مدیریت نشریات علمی, 12(1), pp. 35-42. doi: 10.31661/jbpe.v0i0.1212
Taeb, Shahram, Mosleh-Shiraz, Mohammad Amin, Ghaderi, Abbas, Mortazavi, Seyed Mohammad Javad, Razmkhah, Mahboobeh. Adipose-Derived Mesenchymal Stem Cells Responses to Different Doses of Gamma Radiation. سامانه مدیریت نشریات علمی, 1400; 12(1): 35-42. doi: 10.31661/jbpe.v0i0.1212

Adipose-Derived Mesenchymal Stem Cells Responses to Different Doses of Gamma Radiation

Journal of Biomedical Physics and Engineering
مقاله 4، دوره 12، شماره 1، فروردین و اردیبهشت 2022، صفحه 35-42    اصل مقاله (1.03 M)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.1212
نویسندگان
Shahram Taeb1؛ Mohammad Amin Mosleh-Shiraz1، 2؛ Abbas Ghaderi3، 4؛ Seyed Mohammad Javad Mortazavi5؛ Mahboobeh Razmkhah* 3
1PhD, Ionizing and Non-ionizing Radiation Protection Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2PhD, Department of Radio-oncology, Shiraz University of Medical Sciences, Shiraz, Iran
3PhD, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4PhD, Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5PhD, Department of Medical Physics and Engineering, Shiraz University of Medical Science, Shiraz, Iran
چکیده
Background: The effects of radiation on the cellular compartments of the tumor microenvironment (TME) might be essential in radiotherapy outcomes.
Objective: We aimed to assess the effects of the different doses of gamma irradiation on viability, ABCA1 and MMP-9 expression in adipose-derived mesenchymal stem cells (ASCs) as a critical part of TME.
Material and Methods: In this experimental study, ASCs were extracted from five healthy donors and irradiated with different doses of 5, 10 and 30 Gy of gamma. Then, RNA was extracted from irradiated ASCs and cDNA was synthesized. The viability of ASCs was determined at 24, 48, 72 and 168 h after irradiation using trypan blue staining. The expression of ABCA1 was checked by quantitative real-time (qRT)-PCR technique and the expression of MMP-9 protein was evaluated by western-blot.
Results: Based on our findings, 10 Gy and 30 Gy but not 5 Gy of gamma irradiation significantly decreased the viability of ASCs after 24, 48, 72 and 168 h compared to the non-irradiated cells (p < 0.05). However, a dose of 5 Gy increased ABCA1 in ASCs significantly compared to 10 Gy and 30 Gy (P=0.01 and P=0.02, respectively). In addition, the analysis of western blot data showed that 5 Gy of gamma irradiation significantly increased the expression of MMP-9 in ASCs (P=0.019).
Conclusion: It is concluded that various doses of gamma radiation elicit differential ASCs responses that may lead to different tumor cell reactions to the radiotherapy through bystander effects.
کلیدواژه‌ها
Radiation؛ Cancer؛ Stem Cells؛ Tumor Microenvironment؛ Bystander Effects
سایر فایل های مرتبط با مقاله
مراجع
  1. Reina-Campos M, Shelton PM, Diaz-Meco MT, Moscat J. Metabolic reprogramming of the tumor microenvironment by p62 and its partners. Biochim Biophys Acta Rev Cancer. 2018;1870:88-95. doi: 10.1016/j.bbcan.2018.04.010. PubMed PMID: 29702207. PubMed PMCID: PMC6193563.
  2. Chen ZY, Hu YY, Hu XF, Cheng LX. The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells. J Radiat Res. 2018;59:555-64. doi: 10.1093/jrr/rry048. PubMed PMID: 30010837. PubMed PMCID: PMC6151644.
  3. Aghaalikhani N, Rashtchizadeh N, Shadpour P, Allameh A, Mahmoodi M. Cancer stem cells as a therapeutic target in bladder cancer. J Cell Physiol. 2019;234:3197-206. doi: 10.1002/jcp.26916. PubMed PMID: 30471107.
  4. Zuk PA, Zhu M, Mizuno H, Huang J, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7:211-28. doi: 10.1089/107632701300062859. PubMed PMID: 11304456.
  5. Seo BM, Miura M, Gronthos S, Bartold PM, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004;364:149-55. doi: 10.1016/S0140-6736(04)16627-0. PubMed PMID: 15246727.
  6. Sabatini F, Petecchia L, Tavian M, Jodon De Villeroche V, Rossi GA, Brouty-Boye D. Human bronchial fibroblasts exhibit a mesenchymal stem cell phenotype and multilineage differentiating potentialities. Lab Invest. 2005;85:962-71. doi: 10.1038/labinvest.3700300. PubMed PMID: 15924148.
  7. Shi Y, Du L, Lin L, Wang Y. Tumour-associated mesenchymal stem/stromal cells: emerging therapeutic targets. Nat Rev Drug Discov. 2017;16:35-52. doi: 10.1038/nrd.2016.193. PubMed PMID: 27811929.
  8. Fan W, Li J, Wang Y, Pan J, Li S, Zhu L, et al. CD105 promotes chondrogenesis of synovium-derived mesenchymal stem cells through Smad2 signaling. Biochem Biophys Res Commun. 2016;474:338-44. doi: 10.1016/j.bbrc.2016.04.101. PubMed PMID: 27107692.
  9. Liu J, Kuwabara A, Kamio Y, Hu S, et al. Human Mesenchymal Stem Cell-Derived Microvesicles Prevent the Rupture of Intracranial Aneurysm in Part by Suppression of Mast Cell Activation via a PGE2-Dependent Mechanism. Stem Cells. 2016;34:2943-55. doi: 10.1002/stem.2448. PubMed PMID: 27350036. PubMed PMCID: PMC5287411.
  10. Nwabo Kamdje AH, Kamga PT, Simo RT, et al. Mesenchymal stromal cells’ role in tumor microenvironment: involvement of signaling pathways. Cancer Biol Med. 2017;14:129-41. doi: 10.20892/j.issn.2095-3941.2016.0033. PubMed PMID: 28607804. PubMed PMCID: PMC5444925.
  11. Corsten MF, Shah K. Therapeutic stem-cells for cancer treatment: hopes and hurdles in tactical warfare. Lancet Oncol. 2008;9:376-84. doi: 10.1016/S1470-2045(08)70099-8. PubMed PMID: 18374291.
  12. Tian LL, Yue W, Zhu F, Li S, Li W. Human mesenchymal stem cells play a dual role on tumor cell growth in vitro and in vivo. J Cell Physiol. 2011;226:1860-7. doi: 10.1002/jcp.22511. PubMed PMID: 21442622.
  13. Binnewies M, Roberts EW, Kersten K, Chan V, et al. Understanding the tumor immune microenvironment (TIME) for effective therapy. Nat Med. 2018;24:541-50. doi: 10.1038/s41591-018-0014-x. PubMed PMID: 29686425. PubMed PMCID: PMC5998822.
  14. Arnold KM, Flynn NJ, Raben A, Romak L, et al. The Impact of Radiation on the Tumor Microenvironment: Effect of Dose and Fractionation Schedules. Cancer Growth Metastasis. 2018;11:1179064418761639. doi: 10.1177/1179064418761639. PubMed PMID: 29551910. PubMed PMCID: PMC5846913.
  15. Barker HE, Paget JT, Khan AA, Harrington KJ. The tumour microenvironment after radiotherapy: mechanisms of resistance and recurrence. Nat Rev Cancer. 2015;15:409-25. doi: 10.1038/nrc3958. PubMed PMID: 26105538. PubMed PMCID: PMC4896389.
  16. Jamal M, Rath BH, Williams ES, Camphausen K, Tofilon PJ. Microenvironmental regulation of glioblastoma radioresponse. Clin Cancer Res. 2010;16:6049-59. doi: 10.1158/1078-0432.CCR-10-2435. PubMed PMID: 21037023. PubMed PMCID: PMC3005074.
  17. Gu H, Huang T, Shen Y, Liu Y, Zhou F, Jin Y, et al. Reactive Oxygen Species-Mediated Tumor Microenvironment Transformation: The Mechanism of Radioresistant Gastric Cancer. Oxid Med Cell Longev. 2018;2018:5801209. doi: 10.1155/2018/5801209. PubMed PMID: 29770167. PubMed PMCID: PMC5892229.
  18. Razmkhah M, Jaberipour M, Hosseini A, Safaei A, Khalatbari B, Ghaderi A. Expression profile of IL-8 and growth factors in breast cancer cells and adipose-derived stem cells (ASCs) isolated from breast carcinoma. Cell Immunol. 2010;265:80-5. doi: 10.1016/j.cellimm.2010.07.006. PubMed PMID: 20705284.
  19. Wang Y, Zhu G, Wang J, Chen J. Irradiation alters the differentiation potential of bone marrow mesenchymal stem cells. Mol Med Rep. 2016;13:213-23. doi: 10.3892/mmr.2015.4539. PubMed PMID: 26572960. PubMed PMCID: PMC4686093.
  20. Filion TM, Qiao M, Ghule PN, Mandeville M, et al. Survival responses of human embryonic stem cells to DNA damage. J Cell Physiol. 2009;220:586-92. doi: 10.1002/jcp.21735. PubMed PMID: 19373864. PubMed PMCID: PMC2925401.
  21. Chen Z, Shi T, Zhang L, Zhu P, Deng M, Huang C, et al. Mammalian drug efflux transporters of the ATP binding cassette (ABC) family in multidrug resistance: A review of the past decade. Cancer Lett. 2016;370:153-64. doi: 10.1016/j.canlet.2015.10.010. PubMed PMID: 26499806.
  22. Ingram WJ, Crowther LM, Little EB, Freeman R, et al. ABC transporter activity linked to radiation resistance and molecular subtype in pediatric medulloblastoma. Exp Hematol Oncol. 2013;2:26. doi: 10.1186/2162-3619-2-26. PubMed PMID: 24219920. PubMed PMCID: PMC3851566.
  23. Efferth T, Langguth P. Transport processes of radiopharmaceuticals and-modulators. Radiat Oncol. 2011;6:59. doi: 10.1186/1748-717X-6-59. PubMed PMID: 21645349. PubMed PMCID: PMC3141524.
  24. Leite JC, De Vasconcelos RB, Da Silva SG, De Siqueira-Junior JP, Marques-Santos LF. ATP-binding cassette transporters protect sea urchin gametes and embryonic cells against the harmful effects of ultraviolet light. Mol Reprod Dev. 2014;81:66-83. doi: 10.1002/mrd.22283. PubMed PMID: 24254332.
  25. Ishikawa T, Nakagawa H, Hagiya Y, Nonoguchi N, Miyatake S, Kuroiwa T. Key Role of Human ABC Transporter ABCG2 in Photodynamic Therapy and Photodynamic Diagnosis. Adv Pharmacol Sci. 2010;2010:587306. doi: 10.1155/2010/587306. PubMed PMID: 21188243. PubMed PMCID: PMC3003952.
  26. Yue H, Hu K, Liu W, Jiang J, Chen Y, Wang R. Role of matrix metalloproteinases in radiation-induced lung injury in alveolar epithelial cells of Bama minipigs. Exp Ther Med. 2015;10:1437-44. doi: 10.3892/etm.2015.2658. PubMed PMID: 26622503. PubMed PMCID: PMC4578051.
  27. Zhou Y, Xia L, He ZS, Ouyang W, Z H, Xie CH. Modulation of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in RAW264.7 cells by irradiation. Mol Med Rep. 2010;3:809-13. doi: 10.3892/mmr.2010.326. PubMed PMID: 21472318.
  28. Kunigal S, Lakka SS, Joseph P, Estes N, Rao JS. MMP-9 inhibition downregulates radiation-induced NF-κ activity leading to apoptosis in breast tumors. Clin Cancer Res. 2008;14:3617. doi: 10.1158/1078-0432.CCR-07-2060. PubMed PMID: 18519796. PubMed PMCID: PMC2410036.
  29. Jadhav U, Mohanam S. Response of neuroblastoma cells to ionizing radiation: modulation of in vitro invasiveness and angiogenesis of human microvascular endothelial cells. Int J Oncol. 2006;29:1525-31. PubMed PMID: 17088992. PubMed PMCID: PMC2441915.
  30. Cruet-Hennequart S, Drougard C, Shaw G, Legendre F, Demoor M, Barry F, et al. Radiation-induced alterations of osteogenic and chondrogenic differentiation of human mesenchymal stem cells. PLoS One. 2015;10:e0119334. doi: 10.1371/journal.pone.0119334. PubMed PMID: 25837977. PubMed PMCID: PMC4383487.
آمار
تعداد مشاهده مقاله: 1,699
تعداد دریافت فایل اصل مقاله: 490


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب