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Evaluating the Radiosensitization Effect of Hydroxyapatite Nanoparticles on Human Breast Adenocarcinoma Cell Line and Fibroblast | ||
| Iranian Journal of Medical Sciences | ||
| مقاله 7، دوره 45، شماره 5، آذر 2020، صفحه 368-376 اصل مقاله (1.87 MB) | ||
| نوع مقاله: Original Article(s) | ||
| شناسه دیجیتال (DOI): 10.30476/ijms.2020.46796 | ||
| نویسندگان | ||
Mitra Soleimani 1؛ Fatemeh Elmi2؛ Seyyed Hossein Mousavie Anijdan3؛ Maryam Mitra Elmi  4
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| 1Student Research Committee, Department of Medical Physics, Radiobiology and Protection, Babol University of Medical Sciences, Babol, Iran | ||
| 2Department of Marine Chemistry, School of Marine and Oceanic Sciences, University of Mazandaran, Babolsar, Iran | ||
| 3Department of Radiation Technology, Allied Medicine Faculty, Babol University of Medical Sciences, Babol, Iran | ||
| 4Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran | ||
| چکیده | ||
| Background: Nanohydroxyapatite (nHAP) exhibit anti-proliferative effects on various cancer cells. However, to date, there are only a few studies on the radiosensitization effect of nHAP. The present study aimed to investigate the possible enhancement of the radiosensitization effect of nHAP on human breast adenocarcinoma cancer (MCF-7) and fibroblast. Methods: nHAP was extracted from fish scales using the thermal alkaline method and characterized at Babol University of Medical Sciences (Babol, Iran) in 2017. The anti-proliferative and the radiosensitization effects of nHAP were investigated by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide (MTT), clonogenic assay, and apoptosis assay. MCF-7 cells and fibroblasts were incubated with different concentrations of nHAP and at different periods. The MTT solution was added and the absorbance was measured at 570 nm. The MCF-7 cells were exposed to 0, 1.5, 3.5, and 5 Gy X-ray irradiation and incubated for 10-14 days. The data were compared using the one-way analysis of variance (ANOVA) followed by the post hoc tests (Tukey’s method). Results: The results showed that nHAP significantly inhibited the growth of MCF-7 cells compared with controls (p <0.001), but the difference was not statistically significant for fibroblasts (P=0.686 at 400 µg/mL at 72 hours). After 48 hours, the proliferation of MCF-7 cells and fibroblasts was inhibited by about 81% and 34% at 400 µg/mL concentration, respectively. The radiosensitization enhancement factor for MCF-7 cells and fibroblasts at a dose of 3.5 Gy and 100 μg/mL concentration were 1.87 and 1.3, respectively. Conclusion: nHAP can be considered as a breast cancer radiosensitization agent with limited damage to the surrounding healthy tissue. | ||
| کلیدواژه‌ها | ||
| Radiation-sensitizing agents؛ Radiotherapy؛ Nanoparticles؛ Hydroxyapatites؛ MCF-7 cells | ||
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