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Soleimani, Mitra, Elmi, Fatemeh, Mousavie Anijdan, Seyyed Hossein, Elmi, Maryam Mitra. (1399). Evaluating the Radiosensitization Effect of Hydroxyapatite Nanoparticles on Human Breast Adenocarcinoma Cell Line and Fibroblast. سامانه مدیریت نشریات علمی, 45(5), 368-376. doi: 10.30476/ijms.2020.46796
Mitra Soleimani; Fatemeh Elmi; Seyyed Hossein Mousavie Anijdan; Maryam Mitra Elmi. "Evaluating the Radiosensitization Effect of Hydroxyapatite Nanoparticles on Human Breast Adenocarcinoma Cell Line and Fibroblast". سامانه مدیریت نشریات علمی, 45, 5, 1399, 368-376. doi: 10.30476/ijms.2020.46796
Soleimani, Mitra, Elmi, Fatemeh, Mousavie Anijdan, Seyyed Hossein, Elmi, Maryam Mitra. (1399). 'Evaluating the Radiosensitization Effect of Hydroxyapatite Nanoparticles on Human Breast Adenocarcinoma Cell Line and Fibroblast', سامانه مدیریت نشریات علمی, 45(5), pp. 368-376. doi: 10.30476/ijms.2020.46796
Soleimani, Mitra, Elmi, Fatemeh, Mousavie Anijdan, Seyyed Hossein, Elmi, Maryam Mitra. Evaluating the Radiosensitization Effect of Hydroxyapatite Nanoparticles on Human Breast Adenocarcinoma Cell Line and Fibroblast. سامانه مدیریت نشریات علمی, 1399; 45(5): 368-376. doi: 10.30476/ijms.2020.46796

Evaluating the Radiosensitization Effect of Hydroxyapatite Nanoparticles on Human Breast Adenocarcinoma Cell Line and Fibroblast

Iranian Journal of Medical Sciences
مقاله 7، دوره 45، شماره 5، آذر 2020، صفحه 368-376    اصل مقاله (1.87 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2020.46796
نویسندگان
Mitra Soleimani1؛ Fatemeh Elmi2؛ Seyyed Hossein Mousavie Anijdan3؛ Maryam Mitra Elmi* 4
1Student Research Committee, Department of Medical Physics, Radiobiology and Protection, Babol University of Medical Sciences, Babol, Iran
2Department of Marine Chemistry, School of Marine and Oceanic Sciences, University of Mazandaran, Babolsar, Iran
3Department of Radiation Technology, Allied Medicine Faculty, Babol University of Medical Sciences, Babol, Iran
4Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
چکیده
Background: Nanohydroxyapatite (nHAP) exhibit anti-proliferative effects on various cancer cells. However, to date, there are only a few studies on the radiosensitization effect of nHAP. The present study aimed to investigate the possible enhancement of the radiosensitization effect of nHAP on human breast adenocarcinoma cancer (MCF-7) and fibroblast.
Methods: nHAP was extracted from fish scales using the thermal alkaline method and characterized at Babol University of Medical Sciences (Babol, Iran) in 2017. The anti-proliferative and the radiosensitization effects of nHAP were investigated by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide (MTT), clonogenic assay, and apoptosis assay. MCF-7 cells and fibroblasts were incubated with different concentrations of nHAP and at different periods. The MTT solution was added and the absorbance was measured at 570 nm. The MCF-7 cells were exposed to 0, 1.5, 3.5, and 5 Gy X-ray irradiation and incubated for 10-14 days. The data were compared using the one-way analysis of variance (ANOVA) followed by the post hoc tests (Tukey’s method).
Results: The results showed that nHAP significantly inhibited the growth of MCF-7 cells compared with controls (p <0.001), but the difference was not statistically significant for fibroblasts (P=0.686 at 400 µg/mL at 72 hours). After 48 hours, the proliferation of MCF-7 cells and fibroblasts was inhibited by about 81% and 34% at 400 µg/mL concentration, respectively. The radiosensitization enhancement factor for MCF-7 cells and fibroblasts at a dose of 3.5 Gy and 100 μg/mL concentration were 1.87 and 1.3, respectively.
Conclusion: nHAP can be considered as a breast cancer radiosensitization agent with limited damage to the surrounding healthy tissue.
کلیدواژه‌ها
Radiation-sensitizing agents؛ Radiotherapy؛ Nanoparticles؛ Hydroxyapatites؛ MCF-7 cells
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