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Fakoor, Mohammad Hadi, Owlia, Parviz, Mousavi gargari, Seyed Latif, Sabokbar, Azar. (1399). In-Silico Analysis and Protective Efficacy of the PcrV Recombinant Vaccine against Pseudomonas Aeruginosa in the Burned and PA-Infected BALB/c Mouse Model. سامانه مدیریت نشریات علمی, 17(2), 121-136. doi: 10.22034/iji.2020.85590.1718
Mohammad Hadi Fakoor; Parviz Owlia; Seyed Latif Mousavi gargari; Azar Sabokbar. "In-Silico Analysis and Protective Efficacy of the PcrV Recombinant Vaccine against Pseudomonas Aeruginosa in the Burned and PA-Infected BALB/c Mouse Model". سامانه مدیریت نشریات علمی, 17, 2, 1399, 121-136. doi: 10.22034/iji.2020.85590.1718
Fakoor, Mohammad Hadi, Owlia, Parviz, Mousavi gargari, Seyed Latif, Sabokbar, Azar. (1399). 'In-Silico Analysis and Protective Efficacy of the PcrV Recombinant Vaccine against Pseudomonas Aeruginosa in the Burned and PA-Infected BALB/c Mouse Model', سامانه مدیریت نشریات علمی, 17(2), pp. 121-136. doi: 10.22034/iji.2020.85590.1718
Fakoor, Mohammad Hadi, Owlia, Parviz, Mousavi gargari, Seyed Latif, Sabokbar, Azar. In-Silico Analysis and Protective Efficacy of the PcrV Recombinant Vaccine against Pseudomonas Aeruginosa in the Burned and PA-Infected BALB/c Mouse Model. سامانه مدیریت نشریات علمی, 1399; 17(2): 121-136. doi: 10.22034/iji.2020.85590.1718

In-Silico Analysis and Protective Efficacy of the PcrV Recombinant Vaccine against Pseudomonas Aeruginosa in the Burned and PA-Infected BALB/c Mouse Model

Iranian Journal of Immunology
مقاله 3، دوره 17، شماره 2، شهریور 2020، صفحه 121-136    اصل مقاله (1.59 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2020.85590.1718
نویسندگان
Mohammad Hadi Fakoor1؛ Parviz Owlia* 2؛ Seyed Latif Mousavi gargari3؛ Azar Sabokbar1
1Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2Molecular Microbiology Research Center, Shahed University, Tehran, Iran.
3Department of biology,Shahed University
چکیده
Background: Pseudomonas aeruginosa is considered as the most severe cause of infections in burn patients and pneumonia infections. Objective: To study the protective effects of recombinant protein vaccine harboring the PcrV of P. aeruginosa in the mouse model of burn and respiratory infections. Methods: Recombinant protein vaccine harboring the PcrV was expressed in the E. coli BL-21 strain. Mice were immunized with the purified recombinant protein, and the antibody titer was measured in the sera obtained from the immunized mice. Immunized and control mice were
challenged by active and passive immunization. The microbial counts in the lung, skin, liver, spleen, and kidney were compared with the control mice. Results: Bioinformatics analysis indicated that the PcrV protein was conserved in 1552 clinical and environmental isolates. Also, the isoelectric point (pI), molecular weight, and Grand Average of Hydropathy (GRAVY) score were analyzed. Mice were injected with recombinant protein, and serum from immunized mice reacted strongly with recombinant antigen at a dilution of 1:64000. The survival rate of mice infected with 5xLD50 of the P. aeruginosa increased significantly up to 75% in the standard strains (PAO1 and PAK), and the number of bacteria, especially in the internal organs (kidney, spleen, and liver) significantly reduced compared to the mice immunized with placebo. Conclusions: Our results demonstrated that the PcrV protein could be an effective candidate vaccine for the generation of antibody response against P. aeruginosa infection.
کلیدواژه‌ها
Burned؛ Pseudomonas aeruginosa؛ Recombinant Protein؛ Respiratory؛ Vaccine
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