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Effects of Low-dose Gamma Radiation on Expression of Apoptotic Genes in Rat Peripheral Blood Lymphocyte | ||
| Journal of Biomedical Physics and Engineering | ||
| مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 16 اردیبهشت 1399 اصل مقاله (519.88 K) | ||
| نوع مقاله: Original Research | ||
| نویسندگان | ||
Zh Ghorbani 1؛ R Fardid  2، 3
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| 1MSc, Radiobiology & Radiation Protection, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2PhD, Associate Professor of Medical Physics, Department of radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 3PhD, Associate Professor of Medical Physics, Ionizing and Non-Ionizing Radiation Protection Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| Background: Exposure to high-dose ionizing radiation is known as a human carcinogen factor, but our information about the effects of low-dose ionizing radiation such as occupational exposures is limited. The main concern of scientific community is biological consequences due to low-dose radiations. Objective: This study aims to evaluate the effects of low-dose γ-radiation on expression changes of apoptotic genes (bax and bcl-2) in the rat peripheral blood lymphocytes. Material and Methods: In this experimental study, 42 adult male rats were classified into 6 groups, which was exposed to various doses values ranged from 20 mGy to 1000 mGy by γ-rays from a Co-60 source. Blood samples were provided for analysis of gene expression 24 h after gamma radiation by relative quantitative Reverse Transcription - Polymerase Chain Reaction (RT-PCR). Radiation sensitivity of rat lymphocytes was measured by the bax/bcl-2 ratio as a predictive marker for radio-sensitivity. Results: The results of this study showed that low dose of gamma radiation can induce down-regulation of bax in rat peripheral blood lymphocytes. Despite other mechanisms of cellular radio-protection, changes in expression of these apoptotic genes can be the primary pathway in responses of the lymphocytes radio-protection to the exposure. Our study revealed a significant decrease in the bax/bcl-2 ratio at 50 mGy dose compare to control and the other irradiated groups (p < 0.05). Conclusion: These results suggest that changes in the bax / bcl-2 ratio especially in radiation workers, as a key factor in apoptosis, can be considered as a biological marker in low-dose gamma radiation. | ||
| کلیدواژه‌ها | ||
| Gamma Radiation؛ Real-Time Polymerase Chain Reaction؛ Bcl-2-Associated X Protein؛ Genes, P53؛ Radiation Sensitivity | ||
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آمار تعداد مشاهده مقاله: 121 تعداد دریافت فایل اصل مقاله: 96 |
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