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Ghorbani, Zhila, Fardid, Reza. (1400). Effects of Low-dose Gamma Radiation on Expression of Apoptotic Genes in Rat Peripheral Blood Lymphocyte. سامانه مدیریت نشریات علمی, 11(6), 693-700. doi: 10.31661/jbpe.v0i0.1166
Zhila Ghorbani; Reza Fardid. "Effects of Low-dose Gamma Radiation on Expression of Apoptotic Genes in Rat Peripheral Blood Lymphocyte". سامانه مدیریت نشریات علمی, 11, 6, 1400, 693-700. doi: 10.31661/jbpe.v0i0.1166
Ghorbani, Zhila, Fardid, Reza. (1400). 'Effects of Low-dose Gamma Radiation on Expression of Apoptotic Genes in Rat Peripheral Blood Lymphocyte', سامانه مدیریت نشریات علمی, 11(6), pp. 693-700. doi: 10.31661/jbpe.v0i0.1166
Ghorbani, Zhila, Fardid, Reza. Effects of Low-dose Gamma Radiation on Expression of Apoptotic Genes in Rat Peripheral Blood Lymphocyte. سامانه مدیریت نشریات علمی, 1400; 11(6): 693-700. doi: 10.31661/jbpe.v0i0.1166

Effects of Low-dose Gamma Radiation on Expression of Apoptotic Genes in Rat Peripheral Blood Lymphocyte

Journal of Biomedical Physics and Engineering
مقاله 4، دوره 11، شماره 6، اسفند 2021، صفحه 693-700    اصل مقاله (569.68 K)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.1166
نویسندگان
Zhila Ghorbani1؛ Reza Fardid* 2، 3
1MSc, Radiobiology & Radiation Protection, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2PhD, Associate Professor of Medical Physics, Department of radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3PhD, Associate Professor of Medical Physics, Ionizing and Non-Ionizing Radiation Protection Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Exposure to high-dose ionizing radiation is known as a human carcinogen factor, but our information about the effects of low-dose ionizing radiation such as occupational exposures is limited. The main concern of scientific community is biological consequences due to low-dose radiations.
Objective: This study aims to evaluate the effects of low-dose γ-radiation on expression changes of apoptotic genes (bax and bcl-2) in the rat peripheral blood lymphocytes.
Material and Methods: In this experimental study, 42 adult male rats were classified into 6 groups, which was exposed to various doses values ranged from 20 mGy to 1000 mGy by γ-rays from a Co-60 source. Blood samples were provided for analysis of gene expression 24 h after gamma radiation by relative quantitative Reverse Transcription - Polymerase Chain Reaction (RT-PCR). Radiation sensitivity of rat lymphocytes was measured by the bax/bcl-2 ratio as a predictive marker for radio-sensitivity.
Results: The results of this study showed that low dose of gamma radiation can induce down-regulation of bax in rat peripheral blood lymphocytes. Despite other mechanisms of cellular radio-protection, changes in expression of these apoptotic genes can be the primary pathway in responses of the lymphocytes radio-protection to the exposure. Our study revealed a significant decrease in the bax/bcl-2 ratio at 50 mGy dose compare to control and the other irradiated groups (p < 0.05).
Conclusion: These results suggest that changes in the bax/bcl-2 ratio especially in radiation workers, as a key factor in apoptosis, can be considered as a biological marker in low-dose gamma radiation.
کلیدواژه‌ها
Gamma Radiation؛ Real-Time Polymerase Chain Reaction؛ Bcl-2-Associated X Protein؛ Genes, P53؛ Radiation Sensitivity
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