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Ramezani Ali Akbari, Khadijeh, Fathollahi, Anwar, Hashemi, Seyed Mahmoud, Pouriran, Ramin, Yeganeh, Farshid. (1398). Activity of Dipeptidyl Peptidase-IV/CD26 and Aminopeptidase N/CD13 in Secretome of Mesenchymal Stem Cells after Treatment with LPS and PMA. سامانه مدیریت نشریات علمی, 17(1), 41-51. doi: 10.22034/iji.2020.80293
Khadijeh Ramezani Ali Akbari; Anwar Fathollahi; Seyed Mahmoud Hashemi; Ramin Pouriran; Farshid Yeganeh. "Activity of Dipeptidyl Peptidase-IV/CD26 and Aminopeptidase N/CD13 in Secretome of Mesenchymal Stem Cells after Treatment with LPS and PMA". سامانه مدیریت نشریات علمی, 17, 1, 1398, 41-51. doi: 10.22034/iji.2020.80293
Ramezani Ali Akbari, Khadijeh, Fathollahi, Anwar, Hashemi, Seyed Mahmoud, Pouriran, Ramin, Yeganeh, Farshid. (1398). 'Activity of Dipeptidyl Peptidase-IV/CD26 and Aminopeptidase N/CD13 in Secretome of Mesenchymal Stem Cells after Treatment with LPS and PMA', سامانه مدیریت نشریات علمی, 17(1), pp. 41-51. doi: 10.22034/iji.2020.80293
Ramezani Ali Akbari, Khadijeh, Fathollahi, Anwar, Hashemi, Seyed Mahmoud, Pouriran, Ramin, Yeganeh, Farshid. Activity of Dipeptidyl Peptidase-IV/CD26 and Aminopeptidase N/CD13 in Secretome of Mesenchymal Stem Cells after Treatment with LPS and PMA. سامانه مدیریت نشریات علمی, 1398; 17(1): 41-51. doi: 10.22034/iji.2020.80293

Activity of Dipeptidyl Peptidase-IV/CD26 and Aminopeptidase N/CD13 in Secretome of Mesenchymal Stem Cells after Treatment with LPS and PMA

Iranian Journal of Immunology
مقاله 4، دوره 17، شماره 1، خرداد 2020، صفحه 41-51    اصل مقاله (759.79 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2020.80293
نویسندگان
Khadijeh Ramezani Ali Akbari1؛ Anwar Fathollahi1؛ Seyed Mahmoud Hashemi1؛ Ramin Pouriran2؛ Farshid Yeganeh* 1
1Department of Immunology, School of Medicine, Shahid Behesti University of Medical Sciences, Tehran, Iran
2Department of Pharmacology, School of Medicine, Shahid Behesti University of Medical Sciences, Tehran, Iran
چکیده
Background: Emerging evidence suggests that secretome of mesenchymal stem cells has many anti-inflammatory and regenerative properties, which makes it a suitable candidate for the treatment of autoimmune and degenerative diseases. Dipeptidyl Peptidase-IV (DPP-IV)/CD26 and Aminopeptidase N (APN)/CD13 are ubiquitous ecto-enzymes which can digest various substrates including some chemokines and neuropeptides that are involved in inflammatory conditions. Objective: To evaluate the enzymatic activity of DPP-IV/CD26 and APN/CD13 in MSC conditioned media (MSC-CM). Methods: The MSCs were isolated from the mouse’s abdominal adipose tissues and were cultured without or with preconditioning by adding 2 µg/mL phorbol 12-myristate 13-acetate (PMA) or lipopolysaccharide (LPS). The levels of interleukin-10 (IL-10), nitric oxide (NO), as well as the enzymatic activities of DPP-IV/CD26 and APN/CD13 were measured in MSC-CM. Results: The level of IL-10 and the enzyme activity of APN/CD13 did not show any changes in the MSC-CM of stimulated and non-stimulated cells. However, NO production was increased after treatment by LPS or PMA; nevertheless, the DPP-IV/CD26 activity was decreased in MSC-CM merely following the stimulation of cells with LPS. Conclusion: Our results indicated that MSC‐secretome had DPP-IV/CD26 and APN/CD13 activity. The DPP-IV/CD26 activity was decreased following stimulation of MSCs by toll-like receptor 4 agonist. Further studies are needed to reveal the possible contribution of DPP-IV/CD26 and APN/CD13 in the anti-inflammatory functions of MSC-CM.
کلیدواژه‌ها
Aminopeptidase N؛ CD13؛ CD26؛ Dipeptidyl Peptidase-IV؛ Mesenchymal Stem Cells
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