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Hou, Meiling, Wang, Xiaodong, Lu, Jike, Guo, Xun, Ding, Cong, Liang, Taotao, Ji, Zhenyu, Xie, Peng, Liu, Xin, Kang, Qiaozhen. (1398). TLR agonist rHP-NAP as an Adjuvant of Dendritic Cell-Based Vaccine to Enhance Anti-Melanoma Response. سامانه مدیریت نشریات علمی, 17(1), 14-25. doi: 10.22034/iji.2020.80291
Meiling Hou; Xiaodong Wang; Jike Lu; Xun Guo; Cong Ding; Taotao Liang; Zhenyu Ji; Peng Xie; Xin Liu; Qiaozhen Kang. "TLR agonist rHP-NAP as an Adjuvant of Dendritic Cell-Based Vaccine to Enhance Anti-Melanoma Response". سامانه مدیریت نشریات علمی, 17, 1, 1398, 14-25. doi: 10.22034/iji.2020.80291
Hou, Meiling, Wang, Xiaodong, Lu, Jike, Guo, Xun, Ding, Cong, Liang, Taotao, Ji, Zhenyu, Xie, Peng, Liu, Xin, Kang, Qiaozhen. (1398). 'TLR agonist rHP-NAP as an Adjuvant of Dendritic Cell-Based Vaccine to Enhance Anti-Melanoma Response', سامانه مدیریت نشریات علمی, 17(1), pp. 14-25. doi: 10.22034/iji.2020.80291
Hou, Meiling, Wang, Xiaodong, Lu, Jike, Guo, Xun, Ding, Cong, Liang, Taotao, Ji, Zhenyu, Xie, Peng, Liu, Xin, Kang, Qiaozhen. TLR agonist rHP-NAP as an Adjuvant of Dendritic Cell-Based Vaccine to Enhance Anti-Melanoma Response. سامانه مدیریت نشریات علمی, 1398; 17(1): 14-25. doi: 10.22034/iji.2020.80291

TLR agonist rHP-NAP as an Adjuvant of Dendritic Cell-Based Vaccine to Enhance Anti-Melanoma Response

Iranian Journal of Immunology
مقاله 2، دوره 17، شماره 1، خرداد 2020، صفحه 14-25    اصل مقاله (879.51 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2020.80291
نویسندگان
Meiling Hou1؛ Xiaodong Wang1؛ Jike Lu1؛ Xun Guo1؛ Cong Ding1؛ Taotao Liang1؛ Zhenyu Ji2؛ Peng Xie3؛ Xin Liu* 1؛ Qiaozhen Kang* 1
1School of Life Sciences, Zhengzhou University, Zhengzhou, PR China
2Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, PR China
3Zhengzhou Xinzhiyi Biotechnology Co., Zhengzhou, PR China
چکیده
Background: Melanoma is a common and malignant cutaneous tumor, which is responsible for a large proportion of skin cancer deaths. Dendritic cell (DC)-based vaccines have achieved positive results in the treatment of melanoma because of their ability to induce cytotoxic response to facilitate tumor elimination. Objective: To improve the efficacy of dendritic cell-based vaccines by the adjuvant activity of Helicobacter pylori neutrophil activating protein (HP-NAP), which is a virulence factor of Helicobacter pylori, has been proved as a TLR agonist with effective immunomodulatory activity. Methods: The recombinant HP-NAP (rHP-NAP) was expressed by using prokaryotic expression system. Dendritic cells (DCs) were cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. After treating with rHP-NAP, the maturation of DCs and dendritic cell-based vaccine were assayed by using flow cytometry and qRT-PCR. The activation and proliferation of T cells were measured by FCM, ELISA and MTT methods. The tumor specific cytotoxic response to resistant B16F10 was detected by using lactate dehydrogenase-release assay and qRT-PCR. Results: The recombinant HP-NAP (rHP-NAP), prepared from E. coli prokaryotic expression system, was able to significantly promote the maturation of dendritic cell-based vaccine loaded with tumor cell lysate (TCL) of B16F10 (DC-B16F10-TCL). Furthermore, it effectively induced the activation and proliferation of T cells and tumor specific cytotoxic response to resistant B16F10 melanoma tumor cells. Conclusion: These results suggested that rHP-NAP possesses the potential for use as an adjuvant of dendritic cell-based vaccine in anti-melanoma treatment.
کلیدواژه‌ها
Cytotoxic Response؛ Dendritic Cell-Based Vaccine؛ Melanoma؛ rHP-NAP
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