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Nazari-Vanani, R, Tondro, G H, Dehdari Vais, R, Haghkhah, M, Heli, H, Sattarahmady, N. (1399). Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter. سامانه مدیریت نشریات علمی, 10(3), 307-318. doi: 10.31661/jbpe.v0i0.1912-1018
R Nazari-Vanani; G H Tondro; R Dehdari Vais; M Haghkhah; H Heli; N Sattarahmady. "Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter". سامانه مدیریت نشریات علمی, 10, 3, 1399, 307-318. doi: 10.31661/jbpe.v0i0.1912-1018
Nazari-Vanani, R, Tondro, G H, Dehdari Vais, R, Haghkhah, M, Heli, H, Sattarahmady, N. (1399). 'Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter', سامانه مدیریت نشریات علمی, 10(3), pp. 307-318. doi: 10.31661/jbpe.v0i0.1912-1018
Nazari-Vanani, R, Tondro, G H, Dehdari Vais, R, Haghkhah, M, Heli, H, Sattarahmady, N. Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter. سامانه مدیریت نشریات علمی, 1399; 10(3): 307-318. doi: 10.31661/jbpe.v0i0.1912-1018

Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter

Journal of Biomedical Physics and Engineering
مقاله 8، دوره 10، شماره 3، شهریور 2020، صفحه 307-318    اصل مقاله (781.65 K)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.1912-1018
نویسندگان
R Nazari-Vanani1؛ G H Tondro1؛ R Dehdari Vais1؛ M Haghkhah2؛ H Heli3؛ N Sattarahmady* 3، 4
1MSc, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2PhD, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
3PhD, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4PhD, Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Mycobacterium tuberculosis (MTB) is a pathogen causing tuberculosis (TB) in human, and TB can cause enormous social and economic disruptions. Lateral flow test strips (LFTSs) are inexpensive, portable, disposable, rapid, and easy-to-use analytical tools.
Objective: LFTSs were prepared for the detection of MTB. LFTSs were fabricated using a new specific probe for MTB H37Rv, based on IS6110 sequence gene, and tailed with poly deoxyadenine (dA).
Material and Methods: In this experimental study, to create test and control zones, streptavidin (STP) and a 150-mer dA were dotted on a nitrocellolose membrane. Gold nanoparticles (GNPs) were conjugated with poly deoxythymidine sequence and placed on the conjugate pad. The composition of immersion buffers for sample pad and conjugate pad, running solution, solutions of GNPs-S-dT150 and STP were introduced. DNA genome of MTB and Mycobacterium bovis in clinical samples was amplified with PCR, and then detected by the LFTSs. During the assay, samples were firstly hybridized in two steps and then placed on a conjugate pad in a manner that positive and negative samples provided two and one red lines, respectively, on the detection pad.
Results: After PCR reaction with biotinylated primer, hybridization process with specific MTB probe-dA70-100 toke 10 min, and running process on the strip was performed within 5 min.
Conclusion: We showed that LFTS can discriminate a particular bacteria strain from others. The LFTSs can be redesigned for detection of other pathogenic genomes.
کلیدواژه‌ها
Lateral Flow؛ Test Strip؛ Tuberculosis؛ Nucleic Acid Hybridization؛ Metal Nanoparticles؛ Gold
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