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Rajabi Pour, M, Fardid, R, Zare, T, Kargar Shouroki, F, Mosleh-Shirazi, M A, Behzad Behbahani, A. (1399). Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53. سامانه مدیریت نشریات علمی, 10(2), 225-234. doi: 10.31661/jbpe.v0i0.1273
M Rajabi Pour; R Fardid; T Zare; F Kargar Shouroki; M A Mosleh-Shirazi; A Behzad Behbahani. "Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53". سامانه مدیریت نشریات علمی, 10, 2, 1399, 225-234. doi: 10.31661/jbpe.v0i0.1273
Rajabi Pour, M, Fardid, R, Zare, T, Kargar Shouroki, F, Mosleh-Shirazi, M A, Behzad Behbahani, A. (1399). 'Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53', سامانه مدیریت نشریات علمی, 10(2), pp. 225-234. doi: 10.31661/jbpe.v0i0.1273
Rajabi Pour, M, Fardid, R, Zare, T, Kargar Shouroki, F, Mosleh-Shirazi, M A, Behzad Behbahani, A. Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53. سامانه مدیریت نشریات علمی, 1399; 10(2): 225-234. doi: 10.31661/jbpe.v0i0.1273

Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53

Journal of Biomedical Physics and Engineering
مقاله 14، دوره 10، شماره 2، تیر 2020، صفحه 225-234    اصل مقاله (903.76 K)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.1273
نویسندگان
M Rajabi Pour1، 2؛ R Fardid* 3، 4؛ T Zare5؛ F Kargar Shouroki6؛ M A Mosleh-Shirazi3، 4؛ A Behzad Behbahani7
1MSc, Department of Radiology, School of Paramedicine, Shiraz University of Medical Sciences, Shiraz, Iran
2MSc, Department of Radiology, Hospital Imam Hussein Orzouieh, Kerman University of Medical Sciences, Kerman, Iran
3PhD, Ionizing and Non-ionizing radiation protection research center, School of Paramedicine, Shiraz University of Medical, Sciences, Shiraz, Iran
4PhD, Department of Radiology, School of Paramedicine, Shiraz University of Medical Sciences, Shiraz, Iran
5PhD Student, Department of Medical Physics and Biomedical Engineering, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
6PhD, Occupational Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
7PhD, Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedicine, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Some operating room personnel are occupationally exposed to genotoxic agents such as anesthetic gases and ionizing radiation. Adaptive response, as a defense mechanism, will occur when cells become exposed to a low dose of factors harming DNA (priming dose), which in the subsequent exposure to higher dose of those factors (challenging dose), show more resistance and sensibility..
Objective: The aim of this study was to investigate adaptive response or synergy of ionizing radiation in the operating room personnel exposed to anesthetic gases by evaluation of the relative gene expression changes of effective genes for DNA repair such as Ku80, Ligase1 and P53.
Material and Methods: In this case-control study, 20 operating room personnel and 20 nurses (who were not present in the operating room) as controls were studied. Venous blood samples were drawn from participants. In order to evaluate the adaptive response, a challenging dose of 2Gy gamma radiation was applied to blood samples. Moreover, RNA extraction and cDNA synthesis were performed. Gene expression level was studied by RT-qPCR and compared with the control group.
Results: : Ligase1 and P53 expression in the operating room personnel was significantly higher than that of the control group before irradiation (P˂0.001). Statistically, there was no significant difference in the Ku80 and P53 expression in the operating room personnel before and after irradiation.
Conclusion: Given the findings of this study, exposure to challenging dose of gamma radiation can induce adaptive response in expression of Ku80 and P53 genes in operating room personnel.
کلیدواژه‌ها
Adaptive Response؛ Ionizing Radiation؛ Anesthetic Gases؛ Operating Room Personnel؛ Occupational Exposure؛ DNA Repair؛ Gene Expression؛ RT-qPCR
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