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Kordestani, Zeinab, Shahrokhi-Farjah, Mariam, Yazdi Rouholamini, Seyede Elmeira, Saberi, Arezoo. (1399). Reduced IKK/NF- kB Expression by Nigella Sativa Extract in Breast Cancer. سامانه مدیریت نشریات علمی, 11(2), 150-158. doi: 10.30476/mejc.2019.82140.1059
Zeinab Kordestani; Mariam Shahrokhi-Farjah; Seyede Elmeira Yazdi Rouholamini; Arezoo Saberi. "Reduced IKK/NF- kB Expression by Nigella Sativa Extract in Breast Cancer". سامانه مدیریت نشریات علمی, 11, 2, 1399, 150-158. doi: 10.30476/mejc.2019.82140.1059
Kordestani, Zeinab, Shahrokhi-Farjah, Mariam, Yazdi Rouholamini, Seyede Elmeira, Saberi, Arezoo. (1399). 'Reduced IKK/NF- kB Expression by Nigella Sativa Extract in Breast Cancer', سامانه مدیریت نشریات علمی, 11(2), pp. 150-158. doi: 10.30476/mejc.2019.82140.1059
Kordestani, Zeinab, Shahrokhi-Farjah, Mariam, Yazdi Rouholamini, Seyede Elmeira, Saberi, Arezoo. Reduced IKK/NF- kB Expression by Nigella Sativa Extract in Breast Cancer. سامانه مدیریت نشریات علمی, 1399; 11(2): 150-158. doi: 10.30476/mejc.2019.82140.1059

Reduced IKK/NF- kB Expression by Nigella Sativa Extract in Breast Cancer

Middle East Journal of Cancer
مقاله 3، دوره 11، شماره 2 - شماره پیاپی 42، تیر 2020، صفحه 150-158    اصل مقاله (911.07 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2019.82140.1059
نویسندگان
Zeinab Kordestani1؛ Mariam Shahrokhi-Farjah2؛ Seyede Elmeira Yazdi Rouholamini2؛ Arezoo Saberi* 3
1Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
2Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
3Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
چکیده
Background: Activation of IKK/NF-kB signaling pathway plays a critical role in inflammation-driven tumor progression. Several natural compounds able to inhibit the IKK/NF-kB activation pathway have been shown to either prevent cancer or inhibit cell growth. Extensive studies have been carried out on the Nigella sativa (N. sativa) by many researchers, and its pharmacological activities including anticancer, analgesic, and anti-inflammatory functions have been explored. This study investigated the effect of N. sativa extract on the mRNA level of NFk (p50, RelB) and IKK (IKKA, IKKB) to determine one of the anti-inflammatory mechanisms of N. sativa in breast cancer cells.
Methods: In this experimental study, MCF7 cell line was treated with different concentrations of hydroalcoholic extracts of N. sativa (0, 200, 400,600,800 μg/mL) for 24, 48 and 72 h. Effects of the extract on cell viability and NFk (p50, RelB ) and IKK (IKKA, IKKB) gene expression were analyzed by MTT assay and real time PCR, respectively.
Results: mRNA expression levels of NFk (p50, RelB) and IKK (IKKA, IKKB) in the treatment group were lower than the untreated (control) group. Fold difference (p50, RelB) of gene expressions in treatment groups were statistically significant (P =0.001 and P =0.003) and the fold difference of IKK (IKKA, IKKB) in the treatment groups was lower than that of the untreated groups (P=0.01 and P=0.001).
Conclusion: One possible anti-inflammatory mechanism of N. sativa is associated with the reduction in mRNA levels of NFk (p50, RelB) and IKK (IKKA, IKKB) in breast cancer.
کلیدواژه‌ها
IKK؛ NF-kB؛ Nigella sativa؛ Breast cancer
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