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Nadi, S, Shabestani Monfared, A, Zabihi, E, Mahmoudzadeh, A, Eyvazzadeh, N, Tahamtan, R. (1398). Combined Effect of Iodine Contrast Media, Cisplatin and External Beam Radiotherapy on Anaplastic Thyroid Cancer Cells. سامانه مدیریت نشریات علمی, 9(2), 217-226. doi: 10.31661/jbpe.v0i0.553
S Nadi; A Shabestani Monfared; E Zabihi; A Mahmoudzadeh; N Eyvazzadeh; R Tahamtan. "Combined Effect of Iodine Contrast Media, Cisplatin and External Beam Radiotherapy on Anaplastic Thyroid Cancer Cells". سامانه مدیریت نشریات علمی, 9, 2, 1398, 217-226. doi: 10.31661/jbpe.v0i0.553
Nadi, S, Shabestani Monfared, A, Zabihi, E, Mahmoudzadeh, A, Eyvazzadeh, N, Tahamtan, R. (1398). 'Combined Effect of Iodine Contrast Media, Cisplatin and External Beam Radiotherapy on Anaplastic Thyroid Cancer Cells', سامانه مدیریت نشریات علمی, 9(2), pp. 217-226. doi: 10.31661/jbpe.v0i0.553
Nadi, S, Shabestani Monfared, A, Zabihi, E, Mahmoudzadeh, A, Eyvazzadeh, N, Tahamtan, R. Combined Effect of Iodine Contrast Media, Cisplatin and External Beam Radiotherapy on Anaplastic Thyroid Cancer Cells. سامانه مدیریت نشریات علمی, 1398; 9(2): 217-226. doi: 10.31661/jbpe.v0i0.553

Combined Effect of Iodine Contrast Media, Cisplatin and External Beam Radiotherapy on Anaplastic Thyroid Cancer Cells

Journal of Biomedical Physics and Engineering
مقاله 10، دوره 9، شماره 2، تیر 2019، صفحه 217-226    اصل مقاله (508.71 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.553
نویسندگان
S Nadi1؛ A Shabestani Monfared* 2؛ E Zabihi3؛ A Mahmoudzadeh4؛ N Eyvazzadeh5؛ R Tahamtan6
1MSc Student of Radiobiology and Radiation Protection, Cellular and Molecular Biology Research Centre, Babol University of Medical Sciences, Babol, Iran
2Professor of Medical Physics, Cellular and Molecular Biology Research Centre, Babol University of Medical Sciences, Babol, Iran
3PharmD, Phd, Cellular and Molecular Biology Research Centre, Babol University of Medical Sciences, Babol, Iran
4Phd Immunology, Department of Bioscience and Biotechnology Malek-Ashtar University of Technology. Tehran, Iran
5Phd of medical physic, Radiation Research Center, Faculty of Paramedicine, Aja University of Medical Sciences, Tehran, Iran
6MSc of Radiobiology and Radiation Protection, Babol University of Medical Sciences, Babol, Iran
چکیده
Introduction: The current study investigated the combination of high Z atoms (iodine-, platinium-based drugs) with using low energy irradiation (120kvp) in Anaplastic Thyroid cancer cells.
Material and Methods: For this purpose, eight groups were designed: control (CNT), different concentrations of Iodine contrast media (ICM), irradiation with various doses, Cis-platin (CDDP) with different concentrations, (ICM + CDDP), (ICM + RAD), (CDDP + RAD) and (ICM + CDDP + RAD). The viability was measured by MTT and Colony assay. In MTT assay, the viability of 8305c cells RAD (2 Gy)+ICM (10mg/mL) group was significantly lower than those treated with RAD or ICM alone. CDDP +ICM+RAD group significantly decreased the viability. In colony assay, cells in ICM + RAD (2 Gy) group reduced the number of colonies more significant than RAD group. The difference of colony forming ability between CDDP and CDDP + RAD (2 Gy) was significant. The difference of ICM + CDDP + RAD (2 Gy) and CDDP +RAD (2 Gy) group was significant. All data were statistically analysed using one-way analysis of variance (ANOVA) followed by Chafe’s multi-comparisons tests. All data were presented as mean ± standard deviation (SD) and analysed using statistical package for social sciences (SPSS 16). Significance was considered to be p<0.05.
Results: In MTT assay, the viability of 8305c cells RAD (2 Gy) + ICM (10mg/mL) group was significantly lower than those treated with RAD or ICM alone. CDDP + ICM + RAD group significantly decreased the viability. In colony assay, cells in ICM + RAD (2 Gy) group reduced the number of colonies more significantly than RAD group. The difference of colony forming ability between CDDP and CDDP + RAD (2 Gy) was significant. The difference of ICM + CDDP + RAD (2 Gy) and CDDP + RAD (2 Gy) group was significant.
Conclusion: Exposure of ATC to ICM in the presence of CDDP increases tissue X-rays absorbance by Auger electrons and photo electrons leading to more fatal effects against the tumour.
کلیدواژه‌ها
Anaplastic Thyroid Carcinoma؛ Radiotherapy؛ Cisplatin؛ Iodine Contrast Media؛ Photoelectric
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