LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,149
تعداد مقالات10,518
تعداد مشاهده مقاله45,415,496
تعداد دریافت فایل اصل مقاله11,291,291
Mohd Zainudin, N H, Abdullah, R, Rahman, W N. (1399). Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy. سامانه مدیریت نشریات علمی, 10(3), 319-328. doi: 10.31661/jbpe.v0i0.1135
N H Mohd Zainudin; R Abdullah; W N Rahman. "Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy". سامانه مدیریت نشریات علمی, 10, 3, 1399, 319-328. doi: 10.31661/jbpe.v0i0.1135
Mohd Zainudin, N H, Abdullah, R, Rahman, W N. (1399). 'Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy', سامانه مدیریت نشریات علمی, 10(3), pp. 319-328. doi: 10.31661/jbpe.v0i0.1135
Mohd Zainudin, N H, Abdullah, R, Rahman, W N. Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy. سامانه مدیریت نشریات علمی, 1399; 10(3): 319-328. doi: 10.31661/jbpe.v0i0.1135

Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy

Journal of Biomedical Physics and Engineering
مقاله 9، دوره 10، شماره 3، شهریور 2020، صفحه 319-328    اصل مقاله (823.67 K)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.1135
نویسندگان
N H Mohd Zainudin1، 2؛ R Abdullah3، 4؛ W N Rahman* 5
1PhD student, Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia
2PhD student, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Gong Badak Campus, Kuala Terengganu, Malaysia
3PhD, Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia
4PhD, Department of Nuclear Medicine, Radiotherapy and Oncology, Hospital Universiti Sains Malaysia (HUSM), Kelantan, Malaysia
5PhD, Department of Nuclear Medicine, Radiotherapy and Oncology, Hospital Universiti Sains Malaysia, Kelantan, Malaysia
چکیده
Background: Radiation induced bystander effects (RIBEs) occurs in unirradiated cells exhibiting indirect biological effect as a consequence of signals from other irradiated cells in the population.
Objective: In this study, bystander effects in MCF-7 breast cancer cells and hFOB 1.19 normal osteoblast cells irradiated with gamma emitting HDR Brachytherapy Ir-192 source were investigated.
Material and Methods: In this in-vitro study, bystander effect stimulation was conducted using medium transfer technique of irradiated cells to the non-irradiated bystander cells. Cell viability, reactive oxygen species (ROS) generation and colony forming assay was employed to evaluate the effect.
Results: Results indicate that the exposure to the medium irradiated MCF-7 induced significant bystander killing and decreased the survival fraction of bystander MCF-7 and hFOB from 1.19 to 81.70 % and 65.44 %, respectively. A significant decrease in survival fraction was observed for hFOB 1.19 bystander cells (p < 0.05). We found that the rate of hFOB 1.19 cell growth significantly decreases to 85.5% when added with media from irradiated cells. The ROS levels of bystander cells for both cell lines were observed to have an increase even after 4 h of treatment. Our results suggest the presence of bystander effects in unirradiated cells exposed to the irradiated medium.
Conclusion: These data provide evidence that irradiated MCF-7 breast cancer cells can induce bystander death in unirradiated MCF-7 and hFOB 1.19 bystander cells. Increase in cell death could also be mediated by the ROS generation during the irradiation with HDR brachytherapy.
کلیدواژه‌ها
Breast Cancer؛ Osteoblasts؛ Brachytherapy؛ Bystander Effect؛ Radiation Effect
سایر فایل های مرتبط با مقاله
مراجع
  1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394-424. doi: 10.3322/caac.21492. PubMed PMID: 30207593.
  2. Kauer-Dorner D, Berger D. The Role of Brachytherapy in the Treatment of Breast Cancer. Breast Care (Basel). 2018;13(3):157-161. doi: 10.1159/000489638. PubMed PMID: 30069174. PubMed PMCID: PMC6062670.
  3. Roy S, Devleena TM, Chaudhuri P, Lahiri D, Biswas J. Tumor bed boost in breast cancer: Brachytherapy versus electron beam. Indian J Med Paediatr Oncol. 2013;34(4):257-63. doi: 10.4103/0971-5851.125238. PubMed PMID: 24604954. PubMed PMCID: PMC3932592.
  4. Gliński B, Za̧bek M, Mituś M. Brachytherapy boost in women with early-stage breast cancer treated with breast conserving therapy. Reports of Practical Oncology & Radiotherapy. 2007;12(1):47-51. doi. 10.1016/S1507-1367(10)60040-5.
  5. Baskar R, Dai J, Wenlong N, Yeo R, Yeoh KW. Biological response of cancer cells to radiation treatment. Front Mol Biosci. 2014;1:24. doi: 10.3389/fmolb.2014.00024. PubMed PMID: 25988165. PubMed PMCID: PMC4429645.
  6. Baskar R, Lee KA, Yeo R, Yeoh KW. Cancer and radiation therapy: current advances and future directions. Int J Med Sci. 2012;9(3):193-9. doi: 10.7150/ijms.3635. PubMed PMID: 22408567. PubMed PMCID: PMC3298009.
  7. Lara GG, Andrade GF, Cipreste MF, et al. Protection of normal cells from irradiation bystander effects by silica-flufenamic acid nanoparticles. J Mater Sci Mater Med. 2018;29(8):130. doi: 10.1007/s10856-018-6134-5. PubMed PMID: 30074096.
  8. Marín A, Martín M, Liñán O, Alvarenga F, et al. Bystander effects and radiotherapy. Rep Pract Oncol Radiother. 2014;20(1):12-21. doi: 10.1016/j.rpor.2014.08.004. PubMed PMID: 25535579. PubMed PMCID: PMC4268598.
  9. Mothersill C, Seymour CB. Radiation-induced bystander effects—implications for cancer. Nat Rev Cancer. 2004;4(2):158-64. doi: 10.1038/nrc1277. PubMed PMID: 14964312.
  10. Shao C, Folkard M, Michael BD, Prise KM. Bystander signaling between glioma cells and fibroblasts targeted with counted particles. Int J Cancer. 2005;116(1):45-51. PubMed PMID: 15756683.
  11. Mothersill C, Seymour C. Radiation-induced bystander effects, carcinogenesis and models. Oncogene. 2003;22(45):7028-33. doi: 10.1038/sj.onc.1206882.
  12. Mladenov E, Li F, Zhang L, Klammer H, Iliakis G. Intercellular communication of DNA damage and oxidative status underpin bystander effects. International journal of radiation biology. 2018;94(8):719-26. doi: 1080/09553002.2018.1434323.
  13. Rostami A, Toossi MT, Sazgarnia A, Soleymanifard S. The effect of glucose-coated gold nanoparticles on radiation bystander effect induced in MCF-7 and QUDB cell lines. Radiat Environ Biophys. 2016;55(4):461-466. PubMed PMID: 27613311. doi: 10.1007/s00411-016-0669-y.
  14. Azzam EI, De Toledo SM, Little JB. Oxidative metabolism, gap junctions and the ionizing radiation-induced bystander effect. Oncogene. 2003;22(45):7050-7. doi: 10.1038/sj.onc.1206961.
  15. Iyer R, Lehnert BE. Factors underlying the cell growth-related bystander responses to α particles. Cancer research. 2000;60(5):1290-8. PubMed PMID: 10728689.
  16. Mothersill C, Stamato TD, Perez ML, Cummins R, Mooney R, Seymour CB. Involvement of energy metabolism in the production of ‘bystander effects’ by radiation. Br J Cancer. 2000;82(10):1740-6. PubMed PMID: 10817512. PubMed PMCID: PMC2374513.
  17. Gómez-Millán J, Katz IS, Farias Vde A, Linares-Fernández JL, et al. The importance of bystander effects in radiation therapy in melanoma skin-cancer cells and umbilical-cord stromal stem cells. Radiother Oncol. 2012;102(3):450-8. doi: 10.1016/j.radonc.2011.11.002. PubMed PMID: 22169765.
  18. Zhang D, Zhou T, He F, Rong Y, Lee SH, Wu S, Zuo L. Reactive oxygen species formation and bystander effects in gradient irradiation on human breast cancer cells. Oncotarget. 2016;7(27):41622-41636. doi: 10.18632/oncotarget.9517. PubMed PMID: 27223435. PubMed PMCID: PMC5173083.
  19. Mothersill C, Seymour C. Medium from irradiated human epithelial cells but not human fibroblasts reduces the clonogenic survival of unirradiated cells. Int J Radiat Biol. 1997;71(4):421-7. PubMed PMID: 9154145.
  20. Veldwijk MR, Zhang B, Wenz F, Herskind C. The biological effect of large single doses: a possible role for non-targeted effects in cell inactivation. PLoS One. 2014;9(1):e84991. doi: 10.1371/journal. PubMed PMID: 24465461. PubMed PMCID: PMC3898915.
  21. Shao C, Folkard M, Held KD, Prise KM. Estrogen enhanced cell-cell signaling in breast cancer cells exposed to targeted irradiation. BMC Cancer. 2008;8:184. doi: 10.1186/1471-2407-8-184. PubMed PMID: 18590532. PubMed PMCID: PMC2443807.
  22. Li XF, Zhu GY, Wang JP, Wang Y. Inhibitory effects of autologous γ-irradiated cell conditioned medium on osteoblasts in vitro. Mol Med Rep. 2015;12(1):273-80. doi: 10.3892/mmr.2015.3354. PubMed PMID: 25684548.
  23. Kirolikar S, Prasannan P, Raghuram GV, Pancholi N, Saha T, et al. Prevention of radiation-induced bystander effects by agents that inactivate cell-free chromatin released from irradiated dying cells. Cell Death Disease. 2018;9(12):1142. doi: 10.1038/s41419-018-1181-x.
  24. Lyng FM, Howe OL, McClean B. Reactive oxygen species-induced release of signalling factors in irradiated cells triggers membrane signalling and calcium influx in bystander cells. International journal of radiation biology. 2011;87(7):683-95. doi: 10.3109/09553002.2010.549533.
  25. Jella KK, Moriarty R, McClean B, Byrne HJ, Lyng FM. Reactive oxygen species and nitric oxide signaling in bystander cells. PloS one. 2018;13(4). doi: 10.1371/journal.pone.0195371.
  26. Wang X, Zhang J, Fu J, Wang J, Ye S, Liu W, Shao C. Role of ROS-mediated autophagy in radiation-induced bystander effect of hepatoma cells. Int J Radiat Biol. 2015;91(5):452-8. doi: 10.3109/09553002.2015.1012308. PubMed PMID: 25651038.
  27. Saran M, Bors W. Signalling by O2−• and NO•: how far can either radical, or any specific reaction product, transmit a message under in vivo conditions?. Chemico-biological interactions. 1994;90(1):35-45. doi: 10.1016/0009-2797(94)90109-0.
  28. Yang S, Xu J, Shao W, Geng C, Li J, et al. Radiation-induced bystander effects in A549 cells exposed to 6 MV X-rays. Cell Biochem Biophys. 2015;72(3):877-82. doi: 10.1007/s12013-015-0555-2. PubMed PMID: 25686868.
  29. Zhou H, Ivanov V, Lien Y, Davidson M, Hei T.Mitochondrial Function and NF-κB Mediated Signaling in Radiation-Induced Bystander Effects. Cancer Res. 2008;68(7): 2233–2240. doi: 10.1158/0008-5472.CAN-07-5278. PMCID: PMC3715144.
  30. Desai S, Kumar A, Laskar S, Pandey BN. Cytokine profile of conditioned medium from human tumor cell lines after acute and fractionated doses of gamma radiation and its effect on survival of bystander tumor cells. Cytokine. 2013;61(1):54-62. doi: 10.1016/j.cyto.2012.08.022. PubMed PMID: 23022376.
آمار
تعداد مشاهده مقاله: 2,076
تعداد دریافت فایل اصل مقاله: 730


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب