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Mohd Zainudin, N H, Abdullah, R, Rahman, W N. (1399). Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy. سامانه مدیریت نشریات علمی, 10(3), 319-328. doi: 10.31661/jbpe.v0i0.1135
N H Mohd Zainudin; R Abdullah; W N Rahman. "Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy". سامانه مدیریت نشریات علمی, 10, 3, 1399, 319-328. doi: 10.31661/jbpe.v0i0.1135
Mohd Zainudin, N H, Abdullah, R, Rahman, W N. (1399). 'Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy', سامانه مدیریت نشریات علمی, 10(3), pp. 319-328. doi: 10.31661/jbpe.v0i0.1135
Mohd Zainudin, N H, Abdullah, R, Rahman, W N. Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy. سامانه مدیریت نشریات علمی, 1399; 10(3): 319-328. doi: 10.31661/jbpe.v0i0.1135

Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy

Journal of Biomedical Physics and Engineering
مقاله 9، دوره 10، شماره 3، مرداد و شهریور 2020، صفحه 319-328    اصل مقاله (823.67 K)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.1135
نویسندگان
N H Mohd Zainudin1، 2؛ R Abdullah3، 4؛ W N Rahman* 5
1PhD student, Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia
2PhD student, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Gong Badak Campus, Kuala Terengganu, Malaysia
3PhD, Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia
4PhD, Department of Nuclear Medicine, Radiotherapy and Oncology, Hospital Universiti Sains Malaysia (HUSM), Kelantan, Malaysia
5PhD, Department of Nuclear Medicine, Radiotherapy and Oncology, Hospital Universiti Sains Malaysia, Kelantan, Malaysia
چکیده
Background: Radiation induced bystander effects (RIBEs) occurs in unirradiated cells exhibiting indirect biological effect as a consequence of signals from other irradiated cells in the population.
Objective: In this study, bystander effects in MCF-7 breast cancer cells and hFOB 1.19 normal osteoblast cells irradiated with gamma emitting HDR Brachytherapy Ir-192 source were investigated.
Material and Methods: In this in-vitro study, bystander effect stimulation was conducted using medium transfer technique of irradiated cells to the non-irradiated bystander cells. Cell viability, reactive oxygen species (ROS) generation and colony forming assay was employed to evaluate the effect.
Results: Results indicate that the exposure to the medium irradiated MCF-7 induced significant bystander killing and decreased the survival fraction of bystander MCF-7 and hFOB from 1.19 to 81.70 % and 65.44 %, respectively. A significant decrease in survival fraction was observed for hFOB 1.19 bystander cells (p < 0.05). We found that the rate of hFOB 1.19 cell growth significantly decreases to 85.5% when added with media from irradiated cells. The ROS levels of bystander cells for both cell lines were observed to have an increase even after 4 h of treatment. Our results suggest the presence of bystander effects in unirradiated cells exposed to the irradiated medium.
Conclusion: These data provide evidence that irradiated MCF-7 breast cancer cells can induce bystander death in unirradiated MCF-7 and hFOB 1.19 bystander cells. Increase in cell death could also be mediated by the ROS generation during the irradiation with HDR brachytherapy.
کلیدواژه‌ها
Breast Cancer؛ Osteoblasts؛ Brachytherapy؛ Bystander Effect؛ Radiation Effect
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