پیوندهای مفید
آمار
| تعداد نشریات | 20 |
| تعداد شماره‌ها | 1,149 |
| تعداد مقالات | 10,518 |
| تعداد مشاهده مقاله | 45,415,491 |
| تعداد دریافت فایل اصل مقاله | 11,291,283 |
Effects of Different Storage Media, Temperatures and Times on Osteoblast Preservation in Autogenous Bone Grafts: A Histomorphometrical Analysis | ||
| Journal of Dentistry | ||
| مقاله 9، دوره 21، شماره 3 - شماره پیاپی 68، آذر 2020، صفحه 225-233 اصل مقاله (660.81 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/dentjods.2020.81808.0 | ||
| نویسندگان | ||
| Hamidreza Arabiun1؛ Hossein Bordbar2؛ Seifollah Dehghani Nazhvani3؛ Reyhaneh Ebrahimi4؛ Ehsan Aliabadi1؛ Ilnaz Ghanbari* 5 | ||
| 1Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Dept. of Anatomical Sciences, Histomorphometry, Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 3Dept. of Clinical Science, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 4Dept. of Periodontology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| 5Postgraduate Student, Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| چکیده | ||
| Statement of the Problem: Autogenous bone graft is the gold standard for bone reconstruction. osteogenic cells must be kept viable in graft for a successful procedure. In extracorporeal preservation of grafts during surgery three different factors may influence the quality of grafts. These factors include temperature, storage medium and time period. Purpose: In this study we evaluated the effects of different storage media, temperatures and times on osteoblast count in autogenous bone grafts, preserved extracorporeally. Materials and Method: Samples were obtained from iliac crest region in a goat. The grafts were preserved in 36 groups of different storage time, temperature and medium. Samples were histomorphometrically analysed to determine osteoblast count as the criteria of graft quality. Results: In almost all samples room temperature was the most and incubator was the least favorable storage temperatures. In grafts preserved in room temperature no difference was noted between normal saline and ringer lactate solution and in almost all of the samples autologous blood and dry environment were more favorable media than ringer lactate solution. The effects of time period of storage highly depended on the combination of temperature and solution. Conclusion: the results demonstrated that for preserving as many osteoblasts as possible in bone grafts, the best temperature is room temperature and the least favorable temperature is incubator. Also the best medium for graft storage is blood, which shows better results than normal saline and ringer lactate solution when bone fragments are preserved in room temperature. | ||
| کلیدواژه‌ها | ||
| Autogenous bone graft؛ Osteoblast؛ Extracorporeal preservation؛ Osteogeneic cells؛ Histomorphometrical analysis | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
1. Misch CM. Maxillary autogenous bone grafting. Dent Clin North Am. 2011; 55: 697–713. [PubMed] [Google Scholar]
2. Albrektsson T. The healing of autologous bone grafts after varying degrees of surgical trauma. A microscopic and histochemical study in the rabbit. J Bone Joint Surg Br 1980; 62: 403–410. [PubMed] [Google Scholar]
3. Laursen M, Christensen FB, Bunger C, Lind M. Optimal handling of fresh cancellous bone graft: different peroperative storing techniques evaluated by in vitro osteoblast-like cell metabolism. Acta Orthopaedica Scandinavica. 2003;74: 490–496. [PubMed] [Google Scholar]
4. Steiner M, Ramp WK. Short-term storage of freshly harvested bone. J Oral Maxillofac Surg. 1988; 46: 868–871. [PubMed] [Google Scholar]
5. McAnulty JF. Effect of various short-term storage methods on viability of cancellous bone fragments. Am J Vet Res. 1999; 60: 63–67. [PubMed] [Google Scholar]
6. Dechichi P, Moura CCG, Santana SL, Zanetta-Barbosa D. Histomorphometric analysis of rabbit calvaria bone storage in saline solution versus storage in platelet poor plasma. Int J Oral Maxillofacial Implants. 2007; 22: 905–910. [PubMed] [Google Scholar]
7. Maus U, Andereya S, Gravius S, Helge Siebert C, Schippmann T, Axel Karl Ohnsorge J, et al. How to store autologous bone graft perioperatively: an in vitro study. Archives of Orthopaedic and Trauma Surgery. 2008; 128 [PubMed] [Google Scholar]
8. Rocha FS, Batista JD, Zanetta-Barbosa D, Dechichi P. Effects of different storage media on the regenerative potential of autogenous bone grafts: A histomorphometrical analysis in rabbits. J Oral Implantol. 2013; 39: 635–642. [PubMed] [Google Scholar]
9. Antonenas V, Garvin F, Webb M, Sartor M. Fresh PBSC harvests, but not BM, show temperature-related loss of CD34 viability during storage and transport. Cytotherapy. 2006; 8: 158–165. [PubMed] [Google Scholar]
10. Williams JM, Virdi AS, Pylawka TK, Edwards RB, Markel MD, Cole BJ. Prolonged-fresh preservation of intact whole canine femoral condyles for the potential use as osteochondral allografts. J Orthop Res. 2005; 23: 831–837. [PubMed] [Google Scholar]
11. Charleston JS, Hansen KR, Thyer AC, Charleston LB, Gougeon A, Siebert JR, et al. Estimating human ovarian non-growing follicle number: the application of modern stereology techniques to an old problem. Hum Reprod. 2007; 22: 2103–2110. [PubMed] [Google Scholar] | ||
|
آمار تعداد مشاهده مقاله: 2,324 تعداد دریافت فایل اصل مقاله: 1,759 |
||
