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Ahrami, M, Khatami, M, Heli, H. (1398). Study of Nanofibrils Formation of Fibroin Protein in Specific Thermal and Acidity Conditions. سامانه مدیریت نشریات علمی, 10(1), 39-50. doi: 10.31661/jbpe.v0i0.1092
M Ahrami; M Khatami; H Heli. "Study of Nanofibrils Formation of Fibroin Protein in Specific Thermal and Acidity Conditions". سامانه مدیریت نشریات علمی, 10, 1, 1398, 39-50. doi: 10.31661/jbpe.v0i0.1092
Ahrami, M, Khatami, M, Heli, H. (1398). 'Study of Nanofibrils Formation of Fibroin Protein in Specific Thermal and Acidity Conditions', سامانه مدیریت نشریات علمی, 10(1), pp. 39-50. doi: 10.31661/jbpe.v0i0.1092
Ahrami, M, Khatami, M, Heli, H. Study of Nanofibrils Formation of Fibroin Protein in Specific Thermal and Acidity Conditions. سامانه مدیریت نشریات علمی, 1398; 10(1): 39-50. doi: 10.31661/jbpe.v0i0.1092

Study of Nanofibrils Formation of Fibroin Protein in Specific Thermal and Acidity Conditions

Journal of Biomedical Physics and Engineering
مقاله 5، دوره 10، شماره 1، اردیبهشت 2020، صفحه 39-50    اصل مقاله (1.37 M)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.1092
نویسندگان
M Ahrami1، 2؛ M Khatami3؛ H Heli* 4
1MSc, Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
2MSc, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3MSc, NanoBioeletrochemistry Research Center, Bam University of Medical Sciences, Bam, Iran
4PhD, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Amyloid fibrils are insoluble arranged aggregates of proteins that are fibrillar in structure and related to many diseases (at least 20 types of illnesses) and also create many pathologic conditions. Therefore understanding the circumstance of fibril formation is very important.
Objectives: This study aims to work on fibrillar structure formation of fibroin (as a model protein).
Material and Methods: In this experimental study, fibroin was extracted from bombyx mori silk cocoon, and the concentration was obtained by Bradford method. The protein was incubated in a wide range of times (0 min to 7 days) in specific acidity and thermal conditions (pH=1.6, T=70 °C). The assays of UV-vis spectroscopy with congo red, field emission scanning electron microscopy, transmission electron microscopy, atomic force microscopy and circular dichroism spectroscopy were employed to monitor the fibrillation process.
Results: Fibroin assemblies were formed upon the process of aggregation and fibril formation with a variety of morphology ranging from nanoparticles to elongated fibrils.
Conclusion: The results showed progressive pathway of fibril formation.
کلیدواژه‌ها
Silk Fibroin؛ Circular Dichroism؛ Amyloid Fibrils؛ Microscopy, Electron, Scanning؛ Transmission Electron Microscopy؛ Fibroins
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