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Investigation on the cytotoxicity and apoptosis of citrate based silver nanoparticles, in vitro | ||
| Journal of Health Sciences & Surveillance System | ||
| مقاله 5، دوره 6، شماره 4، دی 2018، صفحه 190-197 اصل مقاله (1.89 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/jhsss.2020.83344.1023 | ||
| نویسندگان | ||
| Laya Ebrahimi1؛ Saeid Hosseinzadeh* 2؛ Maryam Pourmontaseri3؛ Jafar Jalaei3 | ||
| 1Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 2Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University | ||
| 3Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. | ||
| چکیده | ||
| Background: With the advancement of nanotechnology, nanoparticles have been applied in our modern society. However, the hazardous effects of nanoparticles on organisms have not been thoroughly clarified yet. Considering the migration of nanoparticles in food and its subsequent consumption by humans, we have employed normal cell line, the African green monkey kidney cell line (Vero) for evaluation of the cytotoxic activity of the silver nanoparticles. Currently, there are various approaches to perform toxicity tests. In this study, we investigated the effects of citrate-based silver nanoparticles on Vero cells to explore the adverse effects of these nanoparticles. Methods: In an experimental work, to synthetize silver nanoparticles, silver nitrate and citric acid were used. Nanoparticles were further characterized by UV-Visible Spectroscopy, Dynamic Light scattering (DLS) and Scanning Electron Microscopy (SEM). Cells were exposed to various concentrations of the nanoparticles (1.56 to 1000 µg/ml) for 24 h and 48h. The cytotoxic activity and apoptosis were determined using MTT assay and acridine orange/ethidium bromide (AO/EB) staining, respectively. Results: The present study showed a dose-dependent cytotoxicity of the silver nanoparticles with log IC50 values of ~ 10.68 and 2.06 µg/ml for 24 h and 48 h, respectively on Vero cell lines. Analysis by AO/EB staining indicated that the silver nanoparticles induced apoptosis in the cell lines. Conclusion: Silver nanoparticles revealed cytotoxic effects on the Vero cells which was associated with the method of synthesis of silver nanoparticles. | ||
| کلیدواژه‌ها | ||
| Silver nanoparticles؛ Vero cells؛ Cytotoxicity؛ Apoptosis | ||
| مراجع | ||
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1 Lee Y-H, Cheng F-Y, Chiu H-W, Tsai J-C, Fang C-Y, Chen C-W, et al. Cytotoxicity, oxidative stress, apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts. Biomaterials 2014; 35(16): 4706-15 . https://doi.org/10.1016/j.biomaterials.2014.02.021.
2 Senapati S. Biosynthesis and immobilization of nanopaticles and their applications, Thesis (PhD). University of Pune. India. 1 (2005).
3 Verma P, Maheshwari SK. Minimum biofilm eradication concentration (MBEC) assay of Silver and Selenium nanoparticles against biofilm forming Staphylococcus aureus. JMSCR 2017; 5(4): 20213-20222. https://dx.doi.org/10.18535/jmscr/v5i4.77.
4 Kumar S, Mitra A, Halder D. Centella asiatica leaf mediated synthesis of silver nanocolloid and its application as filler in gelatin based antimicrobial nanocomposite film. LWT – Food Sci Technol 2017; 75: 293-300. https://doi.org/10.1016/j.lwt.2016.06.061.
5 Verma P. A review on synthesis and their antibacterial activity of Silver and Selenium nanoparticles against biofilm forming Staphylococcus aureus. World J Pharm Pharmaceut Sci 2015; 4: 652-77.
6 Sotiriou GA, Pratsinis SE. Engineering nanosilver as an antibacterial, biosensor and bioimaging material. Curr Opin Chem Biol 2011; 1(1): 3-10. https://doi.org/10.1016/j.coche.2011.07.001.
7 Eslami M, Bayat M, Nejad ASM, Sabokbar A, Anvar AA. Effect of polymer/nanosilver composite packaging on long-term microbiological status of Iranian saffron (Crocus sativus L.). Saudi J Bio Sci 2016; 23(3): 341-7. https://doi.org/10.1016/j.sjbs.2015.07.004.
8 Bumbudsanpharoke N, Choi J, Ko S. Applications of nanomaterials in food packaging. J Nanosci Nanotechnol 2015; 15(9): 6357-72. https://doi.org/10.1166/jnn.2015.10847 .
9 Castro-Mayorga J, Fabra M, Lagaron J. Stabilized nanosilver based antimicrobial poly (3-hydroxybutyrate-co-3-hydroxyvalerate) nanocomposites of interest in active food packaging. Innov Food Sci Emerg Technol 2016; 33: 524-33. https://doi.org/10.1016/j.ifset.2015.10.019.
10 Huang H, Yang X. Synthesis of polysaccharide-stabilized gold and silver nanoparticles: a green method. Carbohydr Res 2004; 339(15): 2627-31. https://doi.org/10.1016/j.carres.2004.08.005.
11 Wang H, Qiao X, Chen J, Ding S. Preparation of silver nanoparticles by chemical reduction method. Colloids Surf A 2005; (256): 111-115. https://doi.org/10.1016/j.colsurfa.2004.12.058.
12 Long D, Wu G, Chen S. Preparation of oligochitosan stabilized silver nanoparticles by gamma irradiation. Radiat Phys Chem. 2007; 76(7): 1126-31. https://doi.org/10.1016/j.radphyschem.2006.11.001.
13 Zielińska A, Skwarek E, Zaleska A, Gazda M, Hupka J. Preparation of silver nanoparticles with controlled particle size. Procedia Chem. 2009; 1(2): 1560-6. https://doi.org/10.1016/j.proche.2009.11.004.
14 Bumbudsanpharoke N, Ko S. Nano‐food packaging: an overview of market, migration research, and safety regulations, J. Food Sci. 2015; 80(5): R910-R23. https://doi.org/10.1111/1750-3841.12861.
15 Frey EC. Influence of Silver Nanoparticle Surface Charge on Cytotoxic Efficacy against Cancer Cells, Thesis (PhD).University of California. San Luis Obispo. 1(2017).
16 Hernández-Sierra JF, Galicia-Cruz O, Salinas-Acosta A, Ruíz F, Pierdant-Pérez M, Pozos-Guillén A. In vitro cytotoxicity of silver nanoparticles on human periodontal fibroblasts. J Clin Pediatr Dent 2011; 36(1): 37-42. https://doi.org/10.17796/jcpd.36.1.d677647166398886.
17 Movagharnia R, Baghbani-Arani F, Shandiz S, Ataollah S. Cytotoxicity effects of green synthesized silver nanoparticles on human colon cancer (HT29) cells. J Kashan Univ Med Sci—Feyz 2018; 22(1): 31-8. http://feyz.kaums.ac.ir/article-1-3196-en.html.
18 Oberdörster G, Maynard A, Donaldson K, Castranova V, Fitzpatrick J, Ausman K, et al. Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy. Part Fibre Toxicol 2005; 2(1): 8. https://doi.org/10.1186/1743-8977-2-8.
19 Kamali M, Ghorashi SAA, Asadollahi MA. Controllable synthesis of silver nanoparticles using citrate as complexing agent: Characterization of nanopartciles and effect of pH on size and crystallinity. Iran J Chem Chem Eng 2012; 31(4): 21-8.
20 Oćwieja M, Barbasz A, Walas S, Roman M, Paluszkiewicz C. Physicochemical properties and cytotoxicity of cysteine-functionalized silver nanoparticles. Colloid Surface B 2017; 160: 429-37. https://doi.org/10.1016/j.colsurfb.2017.09.042.
21 Oćwieja M, Adamczyk Z. Controlled release of silver nanoparticles from monolayers deposited on PAH covered mica. Langmuir 2013; 29(11): 3546-55. https://doi.org/10.1021/la304855k.
22 Gurunathan S, Han JW, Eppakayala V, Jeyaraj M, Kim J-H. Cytotoxicity of biologically synthesized silver nanoparticles in MDA-MB-231 human breast cancer cells. BioMed Res Int 2013; 2013. https://doi.org/10.1155/2013/535796.
23 Poormontaseri M, Hosseinzadeh S, Shekarforoush SS, Kalantari T. The effects of probiotic Bacillus subtilis on the cytotoxicity of Clostridium perfringens type a in Caco-2 cell culture. BMC Microbiol 2017; 17(1): 150. https://doi.org/10.1186/s12866-017-1051-1.
24 Tyliszczak B, Drabczyk A, Kudłacik-Kramarczyk S, Bialik-Wąs K, Kijkowska R, Sobczak-Kupiec A. Preparation and cytotoxicity of chitosan-based hydrogels modified with silver nanoparticles. Colloid Surface B 2017; 160: 325-30. https://doi.org/10.1016/j.colsurfb.2017.09.044.
25 Ciniglia C, Pinto G, Sansone C, Pollio A. Acridine orange/Ethidium bromide double staining test: A simple in-vitro assay to detect apoptosis induced by phenolic compounds in plant cells. Allelopathy J (2010); 26: 301-308.
26 Saudi A, Rafienia M, Zargar Kharazi A, Salehi H, Zarrabi A, Karevan M. Design and fabrication of poly (glycerol sebacate)-based fibers for neural tissue engineering: Synthesis, electrospinning, and characterization. Polym Advan Technol 2019; 30(6): 1427-40. https://doi.org/10.1002/pat.4575.
27 Devi J, Bhimba B. Anticancer activity of silver nanoparticles synthesized by the seaweed Ulva lactuca in vitro. Sci Rep 2012; 1: 242. http://dx.doi.org/10.4172/scientificreports.242. | ||
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