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Circulating miRNA-30a and miRNA-221 as Novel Biomarkers for the Early Detection of Non-Small-Cell Lung Cancer | ||
| Middle East Journal of Cancer | ||
| مقاله 6، دوره 11، شماره 1 - شماره پیاپی 41، فروردین 2020، صفحه 50-58 اصل مقاله (1.32 M) | ||
| نوع مقاله: Original Article(s) | ||
| شناسه دیجیتال (DOI): 10.30476/mejc.2019.81242.0 | ||
| نویسندگان | ||
| Soad M. Abdel Ghany1؛ Esraa MA. Ali* 1؛ Amr Ahmed2؛ Walaa G. Hozayen2، 3؛ Aliae AR Mohamed-Hussein4؛ Maha Salah Elnaggar5؛ Helal F. Hetta6 | ||
| 1Department of Medical Biochemistry, Faculty of Medicine, Assuit University, Assuit, Egypt | ||
| 2Biotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt | ||
| 3Division of Biochemistry, Department of Chemistry, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt | ||
| 4Department of Pulmonology, Chest, Faculty of Medicine, Assuit University, Assuit, Egypt | ||
| 5Clinical Oncology Department, Assuit University Hospital, Assuit, Egypt | ||
| 6Department of Medical Microbiology and Immunology, Faculty of Medicine, Assuit University, Assuit, Egypt | ||
| چکیده | ||
| Background: Detecting non-small-cell lung cancer at an early stage has become a great challenge due to the lack of a specific non-invasive marker. MicroRNAs are small, non-coding RNA molecules that play a role in carcinogenesis and cancer progression, as indicated by their abnormal expression in the patients’ plasma. Herein, we investigated the plasma level of circulating miRNA-30a and miRNA-221 as non-invasive markers for an early detection of non-small-cell lung cancer. Method: A cross-sectional study was conducted at Assiut University Hospital, Egypt, to investigate miRNA-30a and miRNA-221 expression via quantitative real-time PCR in the plasma of patients with non-small-cell lung cancer (n=70) and healthy controls (n=34). Receiver operating curves were used to evaluate the diagnostic value of miRNA-221 and miRNA-30a in non-small-cell lung cancer. The relationship between both markers and patient clinical parameters was further assessed. Result: Circulating plasma miRNA-30a and miRNA-221 levels were significantly higher in the non-small-cell lung cancer patients compared with those in the healthy controls (P<0.05). There was a significant difference regarding the plasma miRNA-30a level among the three groups (the highest levels were recorded in adenocarcinoma, followed by large cell carcinoma and squamous cell carcinoma). ROC curve analysis of miRNA-30a and miRNA-221 showed that specificity and sensitivity were 60% and 80%, and 40% and 75%, respectively. Conclusion: miRNA-30a and miRNA-221 may be non-invasive biomarkers for early detection and screening or therapeutic targets in patients with NSCLC. Future studies are warranted regarding the use of biomarkers as therapeutic targets. | ||
| کلیدواژه‌ها | ||
| Lung cancer؛ mRNA-30a؛ miRNA-231؛ Non-small-cell lung cancer؛ Biomarker | ||
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