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Roshankhah, Shiva, Arji Rodsari, Babak, Jalili, Cyrus, Salahshoor, Mohammad Reza. (1398). The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line. سامانه مدیریت نشریات علمی, 11(1), 34-41. doi: 10.30476/mejc.2019.78703.0
Shiva Roshankhah; Babak Arji Rodsari; Cyrus Jalili; Mohammad Reza Salahshoor. "The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line". سامانه مدیریت نشریات علمی, 11, 1, 1398, 34-41. doi: 10.30476/mejc.2019.78703.0
Roshankhah, Shiva, Arji Rodsari, Babak, Jalili, Cyrus, Salahshoor, Mohammad Reza. (1398). 'The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line', سامانه مدیریت نشریات علمی, 11(1), pp. 34-41. doi: 10.30476/mejc.2019.78703.0
Roshankhah, Shiva, Arji Rodsari, Babak, Jalili, Cyrus, Salahshoor, Mohammad Reza. The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line. سامانه مدیریت نشریات علمی, 1398; 11(1): 34-41. doi: 10.30476/mejc.2019.78703.0

The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line

Middle East Journal of Cancer
مقاله 4، دوره 11، شماره 1 - شماره پیاپی 41، فروردین 2020، صفحه 34-41    اصل مقاله (1.91 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2019.78703.0
نویسندگان
Shiva Roshankhah1؛ Babak Arji Rodsari1؛ Cyrus Jalili2؛ Mohammad Reza Salahshoor* 1
1Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
2Medical Biology Research Center, Department of Anatomical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran
چکیده
Background: As a major tumor suppressor gene, P53 plays a principal role in the apoptosis of cancer cells. Harmine is a harmal-derived alkaloid with antioxidant and anticancer properties. This study was designed to assess the ability of harmine to express P53 gene and stimulate apoptosis in breast cancer cell line.
Methods: MCF-7 cell line was cultured and treated with harmine. Half maximal inhibitory concentration (IC50) assay was carried out through MMT method following 24 h of treatment. Flow cytometry technique was employed to measure apoptotic cells and real-time PCR was performed to estimate P53 gene expression in tumor cells.
Results: The IC50 for the harmine extract in MCF-7 cells was 30 μM. Harmine administration in all treated groups resulted in a significant increase in apoptosis and P53 gene expression in MCF-7 cells (P < 0.00001).
Conclusions: It seems that harmine administration is able to induce apoptosis in MCF-7 cells through the up-regulation of P53 expression.
کلیدواژه‌ها
Harmine؛ P53 gene؛ MCF-7؛ Apoptosis؛ Cell line
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