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Veisi Malekshahi, Ziba, Hashemi Goradel, Nasser, Shakouri Khomartash, Mehdi, Maleksabet, Amir, kadkhodazadeh, Maryam, Kardar, Gholam Ali, Negahdari, Babak. (1398). CEA Plasmid as Therapeutic DNA Vaccination against Colorectal Cancer. سامانه مدیریت نشریات علمی, 16(3), 235-245. doi: 10.22034/iji.2019.80274
Ziba Veisi Malekshahi; Nasser Hashemi Goradel; Mehdi Shakouri Khomartash; Amir Maleksabet; Maryam kadkhodazadeh; Gholam Ali Kardar; Babak Negahdari. "CEA Plasmid as Therapeutic DNA Vaccination against Colorectal Cancer". سامانه مدیریت نشریات علمی, 16, 3, 1398, 235-245. doi: 10.22034/iji.2019.80274
Veisi Malekshahi, Ziba, Hashemi Goradel, Nasser, Shakouri Khomartash, Mehdi, Maleksabet, Amir, kadkhodazadeh, Maryam, Kardar, Gholam Ali, Negahdari, Babak. (1398). 'CEA Plasmid as Therapeutic DNA Vaccination against Colorectal Cancer', سامانه مدیریت نشریات علمی, 16(3), pp. 235-245. doi: 10.22034/iji.2019.80274
Veisi Malekshahi, Ziba, Hashemi Goradel, Nasser, Shakouri Khomartash, Mehdi, Maleksabet, Amir, kadkhodazadeh, Maryam, Kardar, Gholam Ali, Negahdari, Babak. CEA Plasmid as Therapeutic DNA Vaccination against Colorectal Cancer. سامانه مدیریت نشریات علمی, 1398; 16(3): 235-245. doi: 10.22034/iji.2019.80274

CEA Plasmid as Therapeutic DNA Vaccination against Colorectal Cancer

Iranian Journal of Immunology
مقاله 5، دوره 16، شماره 3، آذر 2019، صفحه 235-245    اصل مقاله (830.85 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2019.80274
نویسندگان
Ziba Veisi Malekshahi1؛ Nasser Hashemi Goradel1؛ Mehdi Shakouri Khomartash2؛ Amir Maleksabet3؛ Maryam kadkhodazadeh4؛ Gholam Ali Kardar5؛ Babak Negahdari* 1
1Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2Shahid Fallahi Polyclinic, AJA University of Medical Sciences, Tehran, Iran
3Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
4Department of biotechnology, Pasteur Institute of Iran, Tehran, Iran
5Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Background: Human colorectal cancer cells overexpress carcinoembryonic antigen (CEA). CEA is a glycoprotein which has shown to be a promising vaccine target for immunotherapy against colorectal cancer. Objective: To design a DNA vaccine harboring CEA antigen and evaluate its effect on inducing immunity against colorectal cancer cells in tumor bearing mice. Methods: In the first step the coding sequence of the CEA was cloned into the pcDNA3.1 vector. The mice were injected with the vaccine construct and the immune responses were monitored during the experiment period. The specific IgG anti-CEA, IFN-γ, IL-2 and IL-4 were measured by ELISA and levels of IFN-γ was detected by ELISpot assay. The lymphocyte proliferation was assessed using a 5-bromo-2-deoxyuridine (BrdU) cell proliferation assay kit. Results: Immunization of the mice with the CEA plasmid resulted in stimulation of CEA-specific T cell and antibody responses. The serum level of specific IgG antibodies against CEA was increased in immunized mice. Moreover, the injection of CEA plasmid led to the stimulation of T-helper-1 by increase in the secretion of IFN-γ, IL-2 and lymphocyte proliferation response. Conclusion: As the CEA DNA vaccine displayed encouraging antitumor effects, therefore, we suggest that it can be a potential therapeutic modality for colorectal cancer and is worthy of further investigation.
کلیدواژه‌ها
Carcinoembryonic Antigen؛ Colorectal Cancer؛ DNA Vaccine
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