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Malekjamshidi, Mohammad Reza, Zandi, Hengameh, Eftekhar, Fereshteh. (1398). Prevalence of Extended-Spectrum β-lactamase and Integron Gene Carriage in Multidrug-Resistant Klebsiella Species Isolated from Outpatients in Yazd, Iran. سامانه مدیریت نشریات علمی, 45(1), 23-31. doi: 10.30476/ijms.2019.45334
Mohammad Reza Malekjamshidi; Hengameh Zandi; Fereshteh Eftekhar. "Prevalence of Extended-Spectrum β-lactamase and Integron Gene Carriage in Multidrug-Resistant Klebsiella Species Isolated from Outpatients in Yazd, Iran". سامانه مدیریت نشریات علمی, 45, 1, 1398, 23-31. doi: 10.30476/ijms.2019.45334
Malekjamshidi, Mohammad Reza, Zandi, Hengameh, Eftekhar, Fereshteh. (1398). 'Prevalence of Extended-Spectrum β-lactamase and Integron Gene Carriage in Multidrug-Resistant Klebsiella Species Isolated from Outpatients in Yazd, Iran', سامانه مدیریت نشریات علمی, 45(1), pp. 23-31. doi: 10.30476/ijms.2019.45334
Malekjamshidi, Mohammad Reza, Zandi, Hengameh, Eftekhar, Fereshteh. Prevalence of Extended-Spectrum β-lactamase and Integron Gene Carriage in Multidrug-Resistant Klebsiella Species Isolated from Outpatients in Yazd, Iran. سامانه مدیریت نشریات علمی, 1398; 45(1): 23-31. doi: 10.30476/ijms.2019.45334

Prevalence of Extended-Spectrum β-lactamase and Integron Gene Carriage in Multidrug-Resistant Klebsiella Species Isolated from Outpatients in Yazd, Iran

Iranian Journal of Medical Sciences
مقاله 4، دوره 45، شماره 1، فروردین 2020، صفحه 23-31    اصل مقاله (586.1 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2019.45334
نویسندگان
Mohammad Reza Malekjamshidi1؛ Hengameh Zandi2؛ Fereshteh Eftekhar* 1
1Departments of Microbiology, Faculty of Life Sciences and Technology, Shahid Beheshti University, Tehran, Iran
2Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
چکیده
Background: Community-acquired infections by multidrug-resistant (MDR), extended-spectrum β-lactamase (ESBL) producing Klebsiella species (Klebsiella spp.) is of major concern worldwide. Antibiotic resistance, production of extended-spectrum β-lactamases (ESBLs), and carbapenemases, as well as the presence of classes 1, 2, and 3 integrons in outpatient isolates of Klebsiella collected from Yazd central laboratory, Yazd, Iran.
Methods: We collected 250 Klebsiella isolates from Yazd central laboratory between August 2015 and October 2017. Antibiotic susceptibility was determined against 18 antibiotics by disc diffusion, and multidrug-resistant isolates were tested for ESBL production by the phenotypic confirmatory test according to CLSI 2017 protocols. The amplification of β-lactamase genes blaSHV, blaTEM, blaCTX-M, blaOXA-48, blaKPC, and blaNDM, classes 1, 2, and 3 integrase genes, was carried out using specific primers and polymerase chain reaction (PCR).
Results: Of the 250 Klebsiella outpatient isolates, 3.6% were K. oxytoca and the rest were K. pneumoniae. Disc diffusion showed that 21 (8.4%) isolates were MDR, 19 (90.4%) of which were ESBL producers including one K. oxytoca. The most prevalent β-lactamase gene was blaSHV followed by blaTEM and blaCTX-M, but blaOXA-48, blaKPC, and blaNDM were not detected. Class 1 integron was detected in 18 out of 21 MDR isolates (85.7%), but classes 2 and 3 were not observed. Two isolates were resistant to carbapenems and harbored blaSHV, blaTEM, and blaCTX-M, as well as class 1 integron.
Conclusion: ESBL production and the presence of multiple β-lactamase genes in MDR community isolates of Klebsiella spp. can have significant implications in terms of the spread of these opportunistic pathogens.
کلیدواژه‌ها
Klebsiella؛ Outpatients؛ Integrons؛ Drug resistance, microbial
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