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In Vitro and In Vivo Evaluation of Poly (3-hydroxybutyrate)/Carbon Nanotubes Electrospun Scaffolds for Periodontal Ligament Tissue Engineering | ||
| Journal of Dentistry | ||
| مقاله 4، دوره 21، شماره 1 - شماره پیاپی 66، خرداد 2020، صفحه 18-30 اصل مقاله (1.18 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/dentjods.2019.77869. | ||
| نویسندگان | ||
| Moein Zarei1؛ Saeed Karbasi2؛ Fatemeh Sari Aslani3؛ Shahrokh Zare1؛ Omid Koohi-Hosseinabad4؛ Nader Tanideh* 5 | ||
| 1Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| 2Dept. of Biomaterials and Tissue Engineering, School of Advance Technology in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. | ||
| 3Molecular Dermatology Research Center, Dept. of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| 4Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| 5Stem Cell Technology Research Center, Dept. of Pharmacology, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| چکیده | ||
| Statement of the Problem: Tissue engineering was an idea and today becomes a potential therapy for several tissues in dentistry such as Periodontal Disease and oral mucosa. Purpose: Periodontal regeneration is one of the earliest clinical disciplines that have achieved the therapeutic application of tissue engineering. The aim of the presence study was to prepare electrospun Poly(3-hydroxybutyrate) (PHB)/1% Carbon nanotubes (CNTs) scaffolds for periodontal regeneration. Materials and Method: 1% w/v of CNTs was added to the polymer solutions and electrospinning. Physical properties of the scaffolds were evaluated by Scanning electron microscopy (SEM) and universal testing machine. Chemical characterization of the scaffolds was also assessed by Fourier-transform infrared spectroscopy (FTIR). Biological properties of the scaffolds were also evaluated in vitro by culturing periodontal ligament stem cells (PDLSCs) on the scaffolds for 10 days and in vivo by Implanting the scaffolds in rat model for 5 weeks. Results: Results showed that the scaffolds were mimicked fibrous connective tissue of the (PDL). CNTs improved the mechanical properties similar to 23-55 years old human PDL. In vitro biocompatibility study showed more attachment and proliferation of the PDLSCs for PHB/1%CNTs scaffolds compared to the PHB controls. In vivo study showed that CNTs in the scaffolds caused mild foreign body type giant cell reaction, moderate vascularization, and mild inflammation. Conclusion: In conclusion, the results showed that the PHB/1%CNTs composite scaffolds may be potentially useful in periodontal regeneration. | ||
| کلیدواژه‌ها | ||
| Tissue engineering؛ Poly (3-hydroxybutyrate)؛ Multi-Walled Carbon nanotubes؛ Scaffold؛ In vitro؛ In vivo؛ Periodontal regeneration | ||
| مراجع | ||
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1. Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. The Lancet. 2004; 364: 149–155. [PubMed] [Google Scholar]
2. Mcculloch CA, Lekic P, Mckee MD. Role of physical forces in regulating the form and function of the periodontal ligament. Periodontology 2000. 2000; 24: 56–72. [PubMed] [Google Scholar]
3. Shimauchi H, Nemoto E, Ishihata H, Shimomura M. Possible functional scaffolds for periodontal regeneration. Japanese Dental Science Review. 2013; 49: 118–130. [Google Scholar]
4. Dillow A, Lowman A. Biomimetic Materials and Design: Biointerfacial Strategies, Tissue Engineering and Targeted Drug Delivery. 1st ed. CRC Press : Boca Raton; 2002. pp. 207–356. [Google Scholar]
5. Wu S, Liu X, Yeung KW, Liu C, Yang X. Biomimetic porous scaffolds for bone tissue engineering. Materials Science and Engineering R Reports. 2014; 80: 1–36. [Google Scholar]
6. Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. 1st ed. The Biomaterials : Silver Jubilee Compendium Elsevier; 2006. pp. 175–189. [Google Scholar]
7. Hasan A, Memic A, Annabi N, Hossain M, Paul A, Dokmeci MR, et al. Electrospun scaffolds for tissue engineering of vascular grafts. Acta Biomaterialia. 2014; 10: 11–25. [PMC free article] [PubMed] [Google Scholar]
8. Jiang T, Carbone EJ, Lo KWH, Laurencin CT. Electrospinning of polymer nanofibers for tissue regeneration. Progress in polymer Science. 2015; 46: 1–24. [Google Scholar]
9. Spasova M, Stoilova O, Manolova N, Rashkov I, Altankov G. Preparation of PLLA/PEG nanofibers by electrospinning and potential applications. Journal of Bioactive and Compatible Polymers. 2007; 22: 62–76. [Google Scholar]
10. Piras A, Chiellini F, Chiellini E, Nikkola L, Ashammakhi N. New multicomponent bioerodible electrospun nanofibers for dual-controlled drug release. Journal of Bioactive and Compatible Polymers. 2008; 23: 423–443. [Google Scholar]
11. Spasova M, Manolova N, Naydenov M, Kuzmanova J, Rashkov I. Electrospun biohybrid materials for plant biocontrol containing chitosan and Trichoderma viride spores. Journal of Bioactive and Compatible Polymers. 2011; 26: 48–55. [Google Scholar]
12. Hollister SJ, Maddox R, Taboas JM. Optimal design and fabrication of scaffolds to mimic tissue properties and satisfy biological constraints. Biomaterials. 2002; 23: 4095–4103. [PubMed] [Google Scholar]
13. Bonartsev A, Myshkina V, Nikolaeva D, Furina E, Makhina T, Livshits V, et al. Biosynthesis, biodegradati- on and application of poly (3-hydroxybutyrate) and its copolymers-natural polyesters produced by diazotrophic bacteria. Communicating Current Research and Educational Topics and Trends in Applied Microbiology. 2007;1: 295–307. [Google Scholar]
14. Kok F, Hasirci V. Tissue Engineering and Novel Delivery Systems. 1th ed. Arcel Dekker : New York, NY, USA; 2004. pp. 543 – 562. [Google Scholar]
15. Hajiali H, Karbasi S, Hosseinalipour M, Rezaie HR. Preparation of a novel biodegradable nanocomposite scaffold based on poly (3-hydroxybutyrate)/bioglass nanoparticles for bone tissue engineering. Journal of Materials Science Materials in Medicine. 2010; 21: 2125–2132. [PubMed] [Google Scholar]
16. Shi Kam NW, Jessop TC, Wender PA, Dai H. Nanotube molecular transporters: internalization of carbon nanotube- protein conjugates into mammalian cells. Journal of the American Chemical Society. 2004; 126: 6850–6851. [PubMed] [Google Scholar]
17. Liu Z, Tabakman S, Welsher K, Dai H. Carbon nanotubes in biology and medicine: in vitro and in vivo detection, imaging and drug delivery. Nano research. 2009; 2: 85–120. [PMC free article] [PubMed] [Google Scholar]
18. Bianco A, Kostarelos K, Partidos CD, Prato M. Biomedical applications of functionalised carbon nanotubes. Chemical Communications. 2005; 5: 571–577. [PubMed] [Google Scholar]
19. Singh R, Pantarotto D, McCarthy D, Chaloin O, Hoebeke J, Partidos CD, et al. Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors. Journal of the American Chemical Society. 2005; 127: 4388–4396. [PubMed] [Google Scholar]
20. Wang H, Li Y, Zuo Y, Li J, Ma S, Cheng L. Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/ polyamide composite scaffolds for bone tissue engineering. Biomaterials. 2007; 28: 3338–3348. [PubMed] [Google Scholar]
21. Kofron M, Cooper Jr J, Kumbar S, Laurencin C. Novel tubular composite matrix for bone repair. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials. 2007; 82: 415–425. [Google Scholar]
22. Jeong J, Moon J, Jeon S, Park J, Alegaonkar P, Yoo J. Mechanical properties of electrospun PVA/MWNTs composite nanofibers. Thin Solid Films. 2007; 515: 5136–5141. [Google Scholar]
23. Kim GM, Michler G, Pötschke P. Deformation processes of ultrahigh porous multiwalled carbon nanotubes/ polycarbonate composite fibers prepared by electrospinning. Polymer. 2005; 46: 7346–7351. [Google Scholar]
24. Zhang H. Electrospun poly (lactic-co-glycolic acid)/ multiwalled carbon nanotubes composite scaffolds for guided bone tissue regeneration. Journal of Bioactive and Compatible Polymers. 2011; 26: 347–362. [Google Scholar]
25. Karbasi S, Zarei M, Foroughi M. Effects of Multi-Wall carbon Nano-Tubes (MWNTs) on structural and mechanical properties of electrospun poly (3-hydroxybutyrate) scaffold for tissue engineering applications. Scientia Iranica Transaction F, Nanotechnology. 2016; 23:3145. [Google Scholar]
26. Ghasemi‐Mobarakeh L, Semnani D, Morshed M. A novel method for porosity measurement of various surface layers of nanofibers mat using image analysis for tissue engineering applications. Journal of applied polymer science. 2007; 106: 2536–2542. [Google Scholar]
27. Gubanski SM, Vlastós AE. Wettability of naturally aged silicon and EPDM composite insulators. IEEE Transactions on Power Delivery. 1990; 5: 1527–1535. [Google Scholar]
28. Bohner M, Lemaitre J. Can bioactivity be tested in vitro with SBF solution? . Biomaterials . 2009;30:2175–2179. [PubMed] [Google Scholar]
29. Beertsen W, McCulloch CA, Sodek J. The periodontal ligament: a unique, multifunctional connective tissue. Periodontology 2000. 1997; 13: 20–40. [PubMed] [Google Scholar]
30. Komatsu K. Mechanical strength and viscoelastic response of the periodontal ligament in relation to structure. Journal of Dental Biomechanics. 2010; 2010:502318. [PMC free article] [PubMed] [Google Scholar]
31. Carletti E, Motta A, Migliaresi C. Scaffolds for tissue engineering and 3D cell culture. Methods Mol Biol. 2011; 695:17 –39. [PubMed] [Google Scholar]
32. Zhang H, Liu J. Electrospun poly (lactic-co-glycolic acid/ acid)/ wool keratin fibrous composite scaffolds potential for bone tissue engineering applications. Journal of Bioactive and Compatible Polymers. 2013; 28: 141–153. [Google Scholar]
33. Deitzel JM, Kleinmeyer J, Harris D, Tan NB. The effect of processing variables on the morphology of electrospun nanofibers and textiles. Polymer. 2001; 42: 261–272. [Google Scholar]
34. Boland ED, Coleman BD, Barnes CP, Simpson DG, Wnek GE, Bowlin GL. Electrospinning polydioxanone for biomedical applications. Acta Biomaterialia. 2005; 1: 115–123. [PubMed] [Google Scholar]
35. Ayres C, Bowlin GL, Henderson SC, Taylor L, Shultz J, Alexander J, et al. Modulation of anisotropy in electrospun tissue-engineering scaffolds: Analysis of fiber alignment by the fast Fourier transform. Biomaterials. 2006; 27: 5524–5534. [PMC free article] [PubMed] [Google Scholar]
36. Naebe M, Lin T, Wang X. Carbon nanotubes reinforced electrospun polymer nanofibres in tech. 1st ed. Rijeka : Croatia; 2010. pp. 309–328. [Google Scholar]
37. Yuan Y, Lee TR. Contact angle and wetting properties. Surface science techniques. 1th ed . Springer: Berlin: Springer: Berlin; 2013. pp. 3–34. [Google Scholar]
38. Landete-Ruiz MD, Martínez-Díez JA, Rodríguez-Pérez MÁ, De Saja JA, Martín-Martínez JM. Improved adhesion of low-density polyethylene/EVA foams using different surface treatments. Journal of adhesion science and technology. 2002; 16: 1073–1101. [Google Scholar]
39. Kokubo T, Takadama H. How useful is SBF in predicting in vivo bone bioactivity? . Biomaterials. 2006;27:2907–2915. [PubMed] [Google Scholar]
40. Hassan MI, Sultana N, Hamdan S. Bioactivity assessment of poly (ε-caprolactone)/ hydroxyapatite electrospun fibers for bone tissue engineering application. Journal of Nanomaterials. 2014; 2014:8. [Google Scholar]
41. de Moraes Porto ICC. Polymer biocompatibility. Polymerization. 1th ed. InTech : Croatia; 2012. pp. 47–63. [Google Scholar]
42. Jaiswal A. Nanofibrous scaffolds for tissue engineering applications. Brazilian Archives of Biology and Technology. 2016; 59:e16150644. [Google Scholar]
43. Zarei M, Karbasi S. Evaluation of the effects of multiwalled carbon nanotubes on electrospun poly (3-hydroxybutirate) scaffold for tissue engineering applications. Journal of Porous Materials. 2018; 25: 259–272. [Google Scholar]
44. Lalwani G, Gopalan A, D'Agati M, Srinivas Sankaran J, Judex S, Qin YX, et al. Porous three‐dimensional carbon nanotube scaffolds for tissue engineering. Journal of Biomedical Materials Research Part A. 2015; 103: 3212–3225. [PMC free article] [PubMed] [Google Scholar]
45. Correa-Duarte MA, Wagner N, Rojas-Chapana J, Morsczeck C, Thie M, Giersig M. Fabrication and biocompatibility of carbon nanotube-based 3D networks as scaffolds for cell seeding and growth. Nano Letters. 2004; 4: 2233–2236. [Google Scholar]
46. Subramanian A, Krishnan UM, Sethuraman S. In vivo biocompatibility of PLGA-polyhexylthiophene nanofiber scaffolds in a rat model. BioMed Research International. 2013; 2013: 1–3. [PMC free article] [PubMed] [Google Scholar]
47. Mikael PE, Amini AR, Basu J, Arellano-Jimenez MJ, Laurencin CT, Sanders MM, et al. Functionalized carbon nanotube reinforced scaffolds for bone regenerative engineering: fabrication, in vitro and in vivo evaluation. Biomedical Materials. 2014; 9:035001. [PubMed] [Google Scholar]
48. Hirata E, Uo M, Takita H, Akasaka T, Watari F, Yokoyama A. Multiwalled carbon nanotube-coating of 3D collagen scaffolds for bone tissue engineering. Carbon. 2011; 49: 3284–3291. [Google Scholar]
49. Luo F, Pan L, Hong G, Wang T, Pei X, Wang J et al. In vitro and in vivo characterization of multi-walled carbon nanotubes/polycaprolactone composite scaffolds for bone tissue engineering applications. Journal of Biomaterials and Tissue Engineering. 2017; 7: 787–797. [Google Scholar]
50. Lam CW, James JT, McCluskey R, Hunter RL. Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation. Toxicological sciences. 2004; 77: 126–134. [PubMed] [Google Scholar]
51. Sato Y, Yokoyama A, Shibata KI, Akimoto Y, Ogino SI, Nodasaka Y, et al. Influence of length on cytotoxicity of multi-walled carbon nanotubes against human acute monocytic leukemia cell line THP-1 in vitro and subcutaneous tissue of rats in vivo. Molecular BioSystems. 2005; 1: 176–182. [PubMed] [Google Scholar] | ||
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