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Rahmani, Shokofeh, Saberzadeh, Jamileh, Takhshid, Mohammad Ali. (1398). The Hydroalcoholic Extract of Saffron Protects PC12 Cells against Aluminum-Induced Cell Death and Oxidative Stress in Vitro. سامانه مدیریت نشریات علمی, 45(1), 59-66. doi: 10.30476/ijms.2019.44971
Shokofeh Rahmani; Jamileh Saberzadeh; Mohammad Ali Takhshid. "The Hydroalcoholic Extract of Saffron Protects PC12 Cells against Aluminum-Induced Cell Death and Oxidative Stress in Vitro". سامانه مدیریت نشریات علمی, 45, 1, 1398, 59-66. doi: 10.30476/ijms.2019.44971
Rahmani, Shokofeh, Saberzadeh, Jamileh, Takhshid, Mohammad Ali. (1398). 'The Hydroalcoholic Extract of Saffron Protects PC12 Cells against Aluminum-Induced Cell Death and Oxidative Stress in Vitro', سامانه مدیریت نشریات علمی, 45(1), pp. 59-66. doi: 10.30476/ijms.2019.44971
Rahmani, Shokofeh, Saberzadeh, Jamileh, Takhshid, Mohammad Ali. The Hydroalcoholic Extract of Saffron Protects PC12 Cells against Aluminum-Induced Cell Death and Oxidative Stress in Vitro. سامانه مدیریت نشریات علمی, 1398; 45(1): 59-66. doi: 10.30476/ijms.2019.44971

The Hydroalcoholic Extract of Saffron Protects PC12 Cells against Aluminum-Induced Cell Death and Oxidative Stress in Vitro

Iranian Journal of Medical Sciences
مقاله 8، دوره 45، شماره 1، فروردین 2020، صفحه 59-66    اصل مقاله (1.17 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2019.44971
نویسندگان
Shokofeh Rahmani1؛ Jamileh Saberzadeh2؛ Mohammad Ali Takhshid* 3
1School of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
2Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Aluminum (Al) exposure is among the environmental risk factors that may involve in the pathogenesis of neurodegenerative diseases. Oxidative stress has a critical role in the Al-induced toxicity. Saffron is a plant with potent radical scavenging and anti-oxidative properties. This investigation was designed to evaluate the possible protective effects of saffron extract (SE) on aluminum maltolate (Almal)-induced oxidative stress and apoptosis in PC12 cell line.
Methods: In this in vitro study, PC12 cells were divided into four groups including control, Almal (500 µM), Almal+SE (50 μg/ml), and Almal+SE (100 μg/ml). After 48 hours of treatment with Almal in the absence and presence of SE, cell viability and apoptosis were determined using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and Annexin V flow cytometry, respectively. Catalase activity was determined as an index of oxidative stress. Statistical analyses were performed using one-way ANOVA (SPSS version 16.0). PResults: Almal decreased the PC12 cells viability dose-dependently (IC50=500µM). Co-treatment of 50 and 100 μg/ml of SE with 500 µM of Al increased cell viability to 79% (P=0.04) and 86% (P=0.02) of the control group, respectively. Al also increased PC12 cells apoptosis and catalase activity to 37 and 2.7 folds of those of the control group (PConclusion: SE has protective effects against Al-induced apoptosis and oxidative stress and may possess therapeutic values in the treatment of Al-neurotoxicity.
کلیدواژه‌ها
Aluminum maltolate؛ Crocus؛ PC12 cells؛ apoptosis؛ Oxidative stress
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