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Isolation and Identification of Lactic Acid Bacteria from a Traditional Fermented Fish Sauce (Mahyaveh) in Fars Province, Iran | ||
International Journal of Nutrition Sciences | ||
مقاله 8، دوره 4، شماره 1، خرداد 2019، صفحه 49-53 اصل مقاله (375.87 K) | ||
نوع مقاله: Original Article | ||
شناسه دیجیتال (DOI): 10.30476/ijns.2019.81437.1005 | ||
نویسندگان | ||
Narjes Karparvar1؛ Hajieh Ghasemian Safaei2؛ Abdollah Derakhshandeh3؛ Fatemeh Hemmati4؛ Seyed Mohammad Mazloomi* 5 | ||
1Department of Nutrition and Food Sciences, Isfahan University of Medical Sciences, Isfahan, Iran | ||
2Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran | ||
3Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
4Student Research Committee, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran | ||
5Department of Food Hygiene and Quality Control, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran | ||
چکیده | ||
Background: Mahyaveh is a fermented fish sauce in southern parts of Iran. Lactic acid bacteria (LAB) are commonly dominant microorganisms in fermented fish products. These bacteria develop organoleptic characteristics of fermented foods and play a significant role in promoting their quality and safety. The present study aimed to identify LAB isolated from Mahyaveh using 16SrDNA gene sequences. Methods: Mahyaveh samples were collected from different regions of Fars province, southern Iran. Then, LAB colonies were isolated using specific media and identified by microscopic observations and biochemical tests. Afterwards, DNA was extracted, PCR was done by general primers of 16S rDNA, and the bacteria were recognized. Results: The 16S rDNA sequence of all isolates was related to Lactobacillus plantarum and Enterococcus faecium type strains. Conclusion: L. plantarum and E. faecium were shown to be prevalent LAB strains that could be used as starters in Mahyaveh fermentation in southern Iran. | ||
کلیدواژهها | ||
Lactic acid bacteria؛ Lactobacillus plantarum؛ Enterococcus faecium؛ Fermented fish sauce؛ Iran | ||
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