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Bahmani, Taraneh, Sharifzadeh, Sedigheh, Tamaddon, Gholamhossein, Farzadfard, Ehsan, Zare, Farahnaz, Fadaie, Milad, Alizadeh, Marzieh, Hadi, Mahdieh, Ranjbaran, Reza, Mosleh-Shirazi, Mohammad Amin, Behzad Behbahani, Abbas. (1400). Mitochondrial Targeted Peptide (KLAKLAK)2, and its Synergistic Radiotherapy Effects on Apoptosis of Radio Resistant Human Monocytic Leukemia Cell Line. سامانه مدیریت نشریات علمی, 11(2), 229-238. doi: 10.31661/jbpe.v0i0.905
Taraneh Bahmani; Sedigheh Sharifzadeh; Gholamhossein Tamaddon; Ehsan Farzadfard; Farahnaz Zare; Milad Fadaie; Marzieh Alizadeh; Mahdieh Hadi; Reza Ranjbaran; Mohammad Amin Mosleh-Shirazi; Abbas Behzad Behbahani. "Mitochondrial Targeted Peptide (KLAKLAK)2, and its Synergistic Radiotherapy Effects on Apoptosis of Radio Resistant Human Monocytic Leukemia Cell Line". سامانه مدیریت نشریات علمی, 11, 2, 1400, 229-238. doi: 10.31661/jbpe.v0i0.905
Bahmani, Taraneh, Sharifzadeh, Sedigheh, Tamaddon, Gholamhossein, Farzadfard, Ehsan, Zare, Farahnaz, Fadaie, Milad, Alizadeh, Marzieh, Hadi, Mahdieh, Ranjbaran, Reza, Mosleh-Shirazi, Mohammad Amin, Behzad Behbahani, Abbas. (1400). 'Mitochondrial Targeted Peptide (KLAKLAK)2, and its Synergistic Radiotherapy Effects on Apoptosis of Radio Resistant Human Monocytic Leukemia Cell Line', سامانه مدیریت نشریات علمی, 11(2), pp. 229-238. doi: 10.31661/jbpe.v0i0.905
Bahmani, Taraneh, Sharifzadeh, Sedigheh, Tamaddon, Gholamhossein, Farzadfard, Ehsan, Zare, Farahnaz, Fadaie, Milad, Alizadeh, Marzieh, Hadi, Mahdieh, Ranjbaran, Reza, Mosleh-Shirazi, Mohammad Amin, Behzad Behbahani, Abbas. Mitochondrial Targeted Peptide (KLAKLAK)2, and its Synergistic Radiotherapy Effects on Apoptosis of Radio Resistant Human Monocytic Leukemia Cell Line. سامانه مدیریت نشریات علمی, 1400; 11(2): 229-238. doi: 10.31661/jbpe.v0i0.905

Mitochondrial Targeted Peptide (KLAKLAK)2, and its Synergistic Radiotherapy Effects on Apoptosis of Radio Resistant Human Monocytic Leukemia Cell Line

Journal of Biomedical Physics and Engineering
مقاله 12، دوره 11، شماره 2، خرداد و تیر 2021، صفحه 229-238    اصل مقاله (787.2 K)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.905
نویسندگان
Taraneh Bahmani1، 2؛ Sedigheh Sharifzadeh3؛ Gholamhossein Tamaddon3، 4؛ Ehsan Farzadfard1، 2؛ Farahnaz Zare1؛ Milad Fadaie5؛ Marzieh Alizadeh2؛ Mahdieh Hadi3، 4؛ Reza Ranjbaran3؛ Mohammad Amin Mosleh-Shirazi6؛ Abbas Behzad Behbahani* 3
1MSc, Division of Medical Biotechnology, Department of Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2MSc, Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3PhD, Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
4PhD, Division of Medical Biotechnology, Department of Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
5MSc, Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
6PhD, Ionizing and Nonionizing Radiation Protection Research Center, and Department of Radiotherapy and Oncology, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Ionizing radiation plays a significant role in cancer treatment. Despite recent advances in radiotherapy approaches, the existence of irradiation-resistant cancer cells is still a noteworthy challenge. Therefore, developing novel therapeutic approaches are still warranted in order to increase the sensitivity of tumor cells to radiation. Many types of research rely on the role of mitochondria in radiation protection.
Objective: Here, we aimed to target the mitochondria of monocyticleukemia (THP-1) radio-resistant cell line cells by a mitochondrial disrupting peptide, D (KLAKLAK)2, and investigate the synergistic effect of Gamma-irradiation and KLA for tumor cells inhibition in vitro.
Material and Methods: In this experimental study, KLA was delivered into THP-1 cells using a Cell-Penetrating Peptide (CPP).The cells were then exposed to gamma-ray radiation both in the presence and absence of KLA conjugated with CPP. The impacts of KLA, ionizing radiation or combination of both were then evaluated on the cell proliferation and apoptosis of THP-1 cells using MTT assay and flow cytometry, respectively.
Results: The MTT assay indicated the anti-proliferative effects of combined D (KLAKLAK)2 peptide with ionizing radiation on THP-1cells. Moreover, synergetic effects of KLA and ionizing radiation reduced cell viability and consequently enhanced cell apoptosis.
Conclusion: Using KLA peptide in combination with ionizing irradiation increases the anticancer effects of radio-resistant THP-1 cells. Therefore, the combinational therapy of (KLAKLAK)2 and radiation is a promising strategy for cancer treatment the in future.
کلیدواژه‌ها
Combination Therapy؛ Ionizing Radiation؛ Radio-Resistance؛ Mitochondria؛ Pro-Apoptotic Peptide؛ Antimicrobial Peptide؛ Cell survival؛ Flow Cytometry
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